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Literature summary extracted from

  • Deng, W.W.; Wang, R.; Yang, T.; Jiang, L.; Zhang, Z.Z.
    Functional characterization of salicylic acid carboxyl methyltransferase from Camellia sinensis, providing the aroma compound of methyl salicylate during the withering process of white tea (2017), J. Agric. Food Chem., 65, 11036-11045 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.274 gene SAMT, sequence comparisons, regulatory elements in the cloned CsSAMT promoter sequence, real-time quantitative PCR enzyme expression analysis, recombinant expression in Escherichia coli and in Saccharomyces cerevisiae Camellia sinensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.274 additional information
-
 additional information kinetics Camellia sinensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.274 chloroplast
-
 Camellia sinensis 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.274 S-adenosyl-L-methionine + salicylate Camellia sinensis
-
 methyl salicylate + S-adenosyl-L-homocysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.274 Camellia sinensis A0A167V6N5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.274 recombinant enzyme CsSAMT from Escherichia coli and from Saccharomyces cerevisiae Camellia sinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.274 leaf
-
 Camellia sinensis
-
2.1.1.274 additional information the CsSAMT gene expression profiles are quite different in tea organs Camellia sinensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.274 additional information substrate specificity of recombinant enzyme, overview Camellia sinensis ?
-
-
2.1.1.274 S-adenosyl-L-methionine + salicylate
-
 Camellia sinensis methyl salicylate + S-adenosyl-L-homocysteine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.274 CsSAMT
-
 Camellia sinensis
2.1.1.274 salicylic acid carboxyl methyltransferase
-
 Camellia sinensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.274 30
-
-
 Camellia sinensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.1.274 10 40 over 50% of maximal activity within this range Camellia sinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.274 7.5
-
-
 Camellia sinensis

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.274 S-adenosyl-L-methionine
-
 Camellia sinensis

General Information

EC Number General Information Comment Organism
2.1.1.274 physiological function salicylic acid carboxyl methyltransferase activity from Camellia sinensis provides the aroma compound methyl salicylate (MeSA) during the withering process of white tea. During the withering process for white tea producing, MeSA is generated by salicylic acid carboxyl methyltransferase (SAMT) with salicylic acid (SA), and the specific floral scent is formed Camellia sinensis