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Literature summary extracted from

  • Koh, D.W.; Park, M.O.; Choi, S.W.; Lee, B.H.; Yoo, S.H.
    Efficient biocatalytic production of cyclodextrins by combined action of amylosucrase and cyclodextrin glucanotransferase (2016), J. Agric. Food Chem., 64, 4371-4375 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.4 food industry cyclodextrins are frequently utilized chemical substances in the food, pharmaceutical, cosmetics, and chemical industries. An enzymatic process for cyclodextrin production is developed by utilizing sucrose as raw material instead of corn starch. Cyclodextrin glucanotransferase from Paenibacillus macerans is applied to produce the cyclodextrins from linear alpha-(1,4)-glucans, which are obtained by Neisseria polysaccharea amylosucrase treatment on sucrose. The greatest cyclodextrin yield (21.1%, w/w) is achieved from a one-pot dual enzyme reaction at 40°C for 24 h. The maximum level of cyclodextrin production (15.1 mg/ml) is achieved with 0.5 M sucrose in a simultaneous mode of dual enzyme reaction, whereas the reaction with 0.1 M sucrose is the most efficient with regard to conversion yield. Dual enzyme synthesis of cyclodextrins is successfully carried out with no need of starch material. Efficient bioconversion process that does not require the high temperature necessary for starch liquefaction by thermostable alpha-amylase in conventional industrial processing Neisseria polysaccharea
2.4.1.4 industry cyclodextrins are frequently utilized chemical substances in the food, pharmaceutical, cosmetics, and chemical industries. An enzymatic process for cyclodextrin production is developed by utilizing sucrose as raw material instead of corn starch. Cyclodextrin glucanotransferase from Paenibacillus macerans is applied to produce the cyclodextrins from linear alpha-(1,4)-glucans, which are obtained by Neisseria polysaccharea amylosucrase treatment on sucrose. The greatest cyclodextrin yield (21.1%, w/w) is achieved from a one-pot dual enzyme reaction at 40°C for 24 h. The maximum level of cyclodextrin production (15.1 mg/ml) is achieved with 0.5 M sucrose in a simultaneous mode of dual enzyme reaction, whereas the reaction with 0.1 M sucrose is the most efficient with regard to conversion yield. Dual enzyme synthesis of cyclodextrins is successfully carried out with no need of starch material. Efficient bioconversion process that does not require the high temperature necessary for starch liquefaction by thermostable alpha-amylase in conventional industrial processing Neisseria polysaccharea
2.4.1.19 food industry cyclodextrins are frequently utilized chemical substances in the food, pharmaceutical, cosmetics, and chemical industries. An enzymatic process for cyclodextrin production is developed by utilizing sucrose as raw material instead of corn starch. Cyclodextrin glucanotransferase from Paenibacillus macerans is applied to produce the cyclodextrins from linear alpha-(1,4)-glucans, which are obtained by Neisseria polysaccharea amylosucrase treatment on sucrose. The greatest cyclodextrin yield (21.1%, w/w) is achieved from a one-pot dual enzyme reaction at 40°C for 24 h. The maximum level of cyclodextrin production (15.1 mg/ml) is achieved with 0.5 M sucrose in a simultaneous mode of dual enzyme reaction, whereas the reaction with 0.1 M sucrose is the most efficient with regard to conversion yield. Dual enzyme synthesis of cyclodextrins is successfully carried out with no need of starch material. Efficient bioconversion process that does not require the high temperature necessary for starch liquefaction by thermostable alpha-amylase in conventional industrial processing Paenibacillus macerans
2.4.1.19 industry cyclodextrins are frequently utilized chemical substances in the food, pharmaceutical, cosmetics, and chemical industries. An enzymatic process for cyclodextrin production is developed by utilizing sucrose as raw material instead of corn starch. Cyclodextrin glucanotransferase from Paenibacillus macerans is applied to produce the cyclodextrins from linear alpha-(1,4)-glucans, which are obtained by Neisseria polysaccharea amylosucrase treatment on sucrose. The greatest cyclodextrin yield (21.1%, w/w) is achieved from a one-pot dual enzyme reaction at 40°C for 24 h. The maximum level of cyclodextrin production (15.1 mg/ml) is achieved with 0.5 M sucrose in a simultaneous mode of dual enzyme reaction, whereas the reaction with 0.1 M sucrose is the most efficient with regard to conversion yield. Dual enzyme synthesis of cyclodextrins is successfully carried out with no need of starch material. Efficient bioconversion process that does not require the high temperature necessary for starch liquefaction by thermostable alpha-amylase in conventional industrial processing Paenibacillus macerans

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.4 Neisseria polysaccharea Q9ZEU2
-
-
2.4.1.19 Paenibacillus macerans
-
-
-
2.4.1.19 Paenibacillus macerans P04830
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 linear alpha-(1,4)-glucan DP 29 + sucrose
-
 Paenibacillus macerans alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
2.4.1.19 linear alpha-(1,4)-glucan DP 38 + sucrose
-
 Paenibacillus macerans alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
2.4.1.19 linear alpha-(1,4)-glucan DP 44 + sucrose
-
 Paenibacillus macerans alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
2.4.1.19 linear alpha-(1,4)-glucan DP 53,116 + sucrose
-
 Paenibacillus macerans alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
2.4.1.19 linear alpha-(1,4)-glucan DP 65,166 + sucrose
-
 Paenibacillus macerans alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
2.4.1.19 soluble potato starch + sucrose
-
 Paenibacillus macerans alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.19 CGTase
-
 Paenibacillus macerans
2.4.1.19 cyclodextrin glucanotransferase
-
 Paenibacillus macerans