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Literature summary extracted from

  • Zhao, Y.; Wang, N.; Wu, H.; Zhou, Y.; Huang, C.; Luo, J.; Zeng, Z.; Kong, L.
    Structure-based tailoring of the first coumarins-specific bergaptol O-methyltransferase to synthesize bergapten for depigmentation disorder treatment (2020), J. Adv. Res., 21, 57-64 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.69 expressed in Escherichia coli BL21(DE3) cells Peucedanum praeruptorum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.69 in complex with S-adenosyl-L-homocysteine and bergaptol Peucedanum praeruptorum

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.69 I157F/V320I the mutant shows about 4fold increased activity compared to the wild type enzyme Peucedanum praeruptorum
2.1.1.69 I157H the mutant shows strongly reduced activity compared to the wild type enzyme Peucedanum praeruptorum
2.1.1.69 I157Y the mutant shows about 2.4fold increased activity compared to the wild type enzyme Peucedanum praeruptorum
2.1.1.69 I157Y/S265F/V315N the mutant shows severely reduced activity compared to the wild type enzyme Peucedanum praeruptorum
2.1.1.69 I157Y/S265N the mutant shows about 1.5fold increased activity compared to the wild type enzyme Peucedanum praeruptorum
2.1.1.69 L122F the mutant shows reduced activity compared to the wild type enzyme Peucedanum praeruptorum
2.1.1.69 L122H the mutant shows severely reduced activity compared to the wild type enzyme Peucedanum praeruptorum
2.1.1.69 L122H/W261H the mutant shows reduced severely activity compared to the wild type enzyme Peucedanum praeruptorum
2.1.1.69 L122H/W261H/H126F the mutant shows severely reduced activity compared to the wild type enzyme Peucedanum praeruptorum
2.1.1.69 L122H/W261H/H126W the mutant shows severely reduced activity compared to the wild type enzyme Peucedanum praeruptorum
2.1.1.69 L122R inactive Peucedanum praeruptorum
2.1.1.69 M175Y/M316W inactive Peucedanum praeruptorum
2.1.1.69 S265H/V315N inactive Peucedanum praeruptorum
2.1.1.69 S265I the mutant shows about 2.5fold increased activity compared to the wild type enzyme Peucedanum praeruptorum
2.1.1.69 S265N the mutant shows about 1.9fold increased activity compared to the wild type enzyme Peucedanum praeruptorum
2.1.1.69 V315F the mutant shows severely reduced activity compared to the wild type enzyme Peucedanum praeruptorum
2.1.1.69 V320I high-catalytic activity mutant with about 8.5fold increased activity compared to the wild type enzyme Peucedanum praeruptorum
2.1.1.69 V320Y the mutant shows reduced activity compared to the wild type enzyme Peucedanum praeruptorum
2.1.1.69 W261H the mutant shows about 1.9fold increased activity compared to the wild type enzyme Peucedanum praeruptorum
2.1.1.69 W261K/M316Y inactive Peucedanum praeruptorum
2.1.1.69 W261L the mutant shows slightly reduced activity compared to the wild type enzyme Peucedanum praeruptorum
2.1.1.69 Y319F the mutant shows about 5fold increased activity compared to the wild type enzyme Peucedanum praeruptorum
2.1.1.69 Y319R inactive Peucedanum praeruptorum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.69 S-adenosyl-L-methionine + a 5-hydroxyfurocoumarin Peucedanum praeruptorum
-
 S-adenosyl-L-homocysteine + a 5-methoxyfurocoumarin
-
 ?
2.1.1.69 S-adenosyl-L-methionine + bergaptol Peucedanum praeruptorum
-
 S-adenosyl-L-homocysteine + bergapten
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.69 Peucedanum praeruptorum A0A166U5H3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.69 glutathione S-transferase-conjugated affinity resin column chromatography Peucedanum praeruptorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.69 S-adenosyl-L-methionine + a 5-hydroxyfurocoumarin
-
 Peucedanum praeruptorum S-adenosyl-L-homocysteine + a 5-methoxyfurocoumarin
-
 ?
2.1.1.69 S-adenosyl-L-methionine + bergaptol
-
 Peucedanum praeruptorum S-adenosyl-L-homocysteine + bergapten
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.69 homodimer
-
 Peucedanum praeruptorum

Synonyms

EC Number Synonyms Comment Organism
2.1.1.69 bergaptol O-methyltransferase
-
 Peucedanum praeruptorum
2.1.1.69 BMT
-
 Peucedanum praeruptorum