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Literature summary extracted from

  • Wang, Q.; Teng, M.; Li, X.
    Functional and structural characterization of a novel catechol-O-methyltransferase from Schizosaccharomyces pombe (2019), IUBMB Life, 71, 330-339 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.6 D162A loss of S-adenosylmethionine binding Schizosaccharomyces pombe
2.1.1.6 E106A loss of S-adenosylmethionine binding Schizosaccharomyces pombe
2.1.1.6 H163A loss of S-adenosylmethionine binding Schizosaccharomyces pombe
2.1.1.6 I56A dissociation constant of S-adenosylmethionine is similar to wild-type. Residue I56 forms a pair of H-bonds with oxygen in the methionine portion Schizosaccharomyces pombe
2.1.1.6 S86A loss of S-adenosylmethionine binding Schizosaccharomyces pombe
2.1.1.6 V107A significant decrease in S-adenosylmethionine binding Schizosaccharomyces pombe
2.1.1.6 W164A dissociation constant of S-adenosylmethionine is similar to wild-type. Residue W164 forms a T-shaped pi-pi interaction with the adenine ring Schizosaccharomyces pombe

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.6 0.015
-
 esculetin pH 7.5, temperature not specified in the publication Schizosaccharomyces pombe

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.6 Mg2+ absolutely required Schizosaccharomyces pombe

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.6 Schizosaccharomyces pombe O42898
-
-
2.1.1.6 Schizosaccharomyces pombe ATCC 24843 O42898
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.6
-
 Schizosaccharomyces pombe

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.6 S-adenosyl-L-methionine + esculetin
-
 Schizosaccharomyces pombe S-adenosyl-L-homocysteine + scopoletin
-
 ?
2.1.1.6 S-adenosyl-L-methionine + esculetin
-
 Schizosaccharomyces pombe ATCC 24843 S-adenosyl-L-homocysteine + scopoletin
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.6 0.0289
-
 esculetin pH 7.5, temperature not specified in the publication Schizosaccharomyces pombe

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.6 2.105
-
 esculetin pH 7.5, temperature not specified in the publication Schizosaccharomyces pombe