Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Song, H.B.; He, M.; Cai, Z.P.; Huang, K.; Flitsch, S.L.; Liu, L.; Voglmeir, J.
    UDP-glucose 4-epimerase and ?-1,4-galactosyltransferase from the oyster Magallana gigas as valuable biocatalysts for the production of galactosylated products (2018), Int. J. Mol. Sci., 19, 1600 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.90 expressed in Escherichia coli BL21(DE3) cells Crassostrea gigas
2.4.1.133 gene CGI_10013446, transcriptome screening and DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Crassostrea gigas

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.90 Mn2+ 1 mM used in assay conditions Crassostrea gigas

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.133 UDP-alpha-D-galactose + O-beta-D-xylosyl-[protein] Crassostrea gigas
-
 UDP + 4-beta-D-galactosyl-O-beta-D-xylosyl-[protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.90 Crassostrea gigas K1QT40
-
-
2.4.1.133 Crassostrea gigas K1QT40
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.90 Ni-NTA column chromatography Crassostrea gigas
2.4.1.133 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Crassostrea gigas

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.90 UDP-alpha-D-galactose + 4-nitrophenyl xylose
-
 Crassostrea gigas UDP + 4-nitrophenyl Xylbeta(1->4)Gal
-
 ?
2.4.1.133 additional information enzymatic galactosylation reaction using epimerase UDP-glucose 4-epimerase MgUGE (EC 5.1.3.2) and beta-1,4-galactosyltransferase MgGalT7 with 4-nitrophenol xylose and UDP-glucose as substrates, mass spectrometric analyses of the acceptor substrate and the galactosylated reaction product, coupled assay method, evaluation, overview Crassostrea gigas ?
-
-
2.4.1.133 UDP-alpha-D-galactose + O-beta-D-xylosyl-[protein]
-
 Crassostrea gigas UDP + 4-beta-D-galactosyl-O-beta-D-xylosyl-[protein]
-
 ?
2.4.1.133 UDP-galactose + 4-nitrophenyl-alpha-D-xyloside
-
 Crassostrea gigas UDP + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.90 ? x * 38000, SDS-PAGE Crassostrea gigas
2.4.1.90 ? x * 37800, calculated from amino acid sequence Crassostrea gigas
2.4.1.133 ? x * 37800, recombinant His6-tagged enzyme, SDS-PAGE Crassostrea gigas

Synonyms

EC Number Synonyms Comment Organism
2.4.1.90 beta-1,4-galactosyltransferase
-
 Crassostrea gigas
2.4.1.90 GalT7
-
 Crassostrea gigas
2.4.1.133 beta-1,4-galactosyltransferase
-
 Crassostrea gigas
2.4.1.133 beta-1,4-galactosyltransferase 7 UniProt Crassostrea gigas
2.4.1.133 MgGalT7
-
 Crassostrea gigas
2.4.1.133 pNP-xylose-beta-1,4-galactosyltransferase
-
 Crassostrea gigas

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.133 37
-
 assay at Crassostrea gigas

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.133 8
-
 assay at Crassostrea gigas