EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.18 | expressed in Escherichia coli BL21(DE3) cells | Parageobacillus thermoglucosidasius |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.1.18 | A310D | the mutant shows strongly reduced activity compared to the wild type enzyme | Parageobacillus thermoglucosidasius |
2.4.1.18 | A310E | the mutant shows strongly reduced activity compared to the wild type enzyme | Parageobacillus thermoglucosidasius |
2.4.1.18 | A310G | the mutant shows strongly reduced activity compared to the wild type enzyme | Parageobacillus thermoglucosidasius |
2.4.1.18 | A310I | the mutant shows strongly reduced activity compared to the wild type enzyme | Parageobacillus thermoglucosidasius |
2.4.1.18 | A310N | the mutant shows strongly reduced activity compared to the wild type enzyme | Parageobacillus thermoglucosidasius |
2.4.1.18 | A310Q | the mutant shows strongly reduced activity compared to the wild type enzyme | Parageobacillus thermoglucosidasius |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.18 | glycogen | Parageobacillus thermoglucosidasius | - |
glycogen containing alpha-1,6-glucosidic linkages | - |
? | |
2.4.1.18 | glycogen | Parageobacillus thermoglucosidasius STB02 | - |
glycogen containing alpha-1,6-glucosidic linkages | - |
? | |
2.4.1.18 | starch | Parageobacillus thermoglucosidasius | - |
starch containing alpha-1,6-glucosidic linkages | - |
? | |
2.4.1.18 | starch | Parageobacillus thermoglucosidasius STB02 | - |
starch containing alpha-1,6-glucosidic linkages | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.18 | Parageobacillus thermoglucosidasius | A0A068F9H3 | - |
- |
2.4.1.18 | Parageobacillus thermoglucosidasius STB02 | A0A068F9H3 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.18 | HiTrap column chromatography and Superdex S200 gel filtration | Parageobacillus thermoglucosidasius |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.18 | 26 | - |
mutant enzyme A310G, using potato amylopectin type III as substrate, at pH 7.5 and 37°C | Parageobacillus thermoglucosidasius |
2.4.1.18 | 27 | - |
mutant enzyme A310N, using potato amylopectin type III as substrate, at pH 7.5 and 37°C | Parageobacillus thermoglucosidasius |
2.4.1.18 | 27 | - |
mutant enzyme A310Q, using potato amylopectin type III as substrate, at pH 7.5 and 37°C | Parageobacillus thermoglucosidasius |
2.4.1.18 | 30 | - |
mutant enzyme A310I, using potato amylopectin type III as substrate, at pH 7.5 and 37°C | Parageobacillus thermoglucosidasius |
2.4.1.18 | 32 | - |
mutant enzyme A310D, using potato amylopectin type III as substrate, at pH 7.5 and 37°C | Parageobacillus thermoglucosidasius |
2.4.1.18 | 32 | - |
mutant enzyme A310E, using potato amylopectin type III as substrate, at pH 7.5 and 37°C | Parageobacillus thermoglucosidasius |
2.4.1.18 | 54 | - |
mutant enzyme A310N, using potato amylose type III as substrate, at pH 7.5 and 37°C | Parageobacillus thermoglucosidasius |
2.4.1.18 | 58 | - |
mutant enzyme A310G, using potato amylose type III as substrate, at pH 7.5 and 37°C | Parageobacillus thermoglucosidasius |
2.4.1.18 | 62 | - |
mutant enzyme A310I, using potato amylose type III as substrate, at pH 7.5 and 37°C | Parageobacillus thermoglucosidasius |
2.4.1.18 | 62 | - |
mutant enzyme A310Q, using potato amylose type III as substrate, at pH 7.5 and 37°C | Parageobacillus thermoglucosidasius |
2.4.1.18 | 67 | - |
mutant enzyme A310E, using potato amylose type III as substrate, at pH 7.5 and 37°C | Parageobacillus thermoglucosidasius |
2.4.1.18 | 70 | - |
mutant enzyme A310D, using potato amylose type III as substrate, at pH 7.5 and 37°C | Parageobacillus thermoglucosidasius |
2.4.1.18 | 299 | - |
wild type enzyme, using potato amylose type III as substrate, at pH 7.5 and 37°C | Parageobacillus thermoglucosidasius |
2.4.1.18 | 384 | - |
wild type enzyme, using potato amylopectin type III as substrate, at pH 7.5 and 37°C | Parageobacillus thermoglucosidasius |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.18 | glycogen | - |
Parageobacillus thermoglucosidasius | glycogen containing alpha-1,6-glucosidic linkages | - |
? | |
2.4.1.18 | glycogen | - |
Parageobacillus thermoglucosidasius STB02 | glycogen containing alpha-1,6-glucosidic linkages | - |
? | |
2.4.1.18 | potato amylopectin type III | - |
Parageobacillus thermoglucosidasius | potato amylopectin type III containing alpha-1,6-glucosidic linkages | - |
? | |
2.4.1.18 | potato amylopectin type III | - |
Parageobacillus thermoglucosidasius STB02 | potato amylopectin type III containing alpha-1,6-glucosidic linkages | - |
? | |
2.4.1.18 | potato amylose type III | - |
Parageobacillus thermoglucosidasius | potato amylose type III containing alpha-1,6-glucosidic linkages | - |
? | |
2.4.1.18 | potato amylose type III | - |
Parageobacillus thermoglucosidasius STB02 | potato amylose type III containing alpha-1,6-glucosidic linkages | - |
? | |
2.4.1.18 | starch | - |
Parageobacillus thermoglucosidasius | starch containing alpha-1,6-glucosidic linkages | - |
? | |
2.4.1.18 | starch | - |
Parageobacillus thermoglucosidasius STB02 | starch containing alpha-1,6-glucosidic linkages | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.18 | ? | x * 75000, SDS-PAGE | Parageobacillus thermoglucosidasius |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.18 | GBE | - |
Parageobacillus thermoglucosidasius |