Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pratap, S.; Kesari, P.; Yadav, R.; Dev, A.; Narwal, M.; Kumar, P.
    Acyl chain preference and inhibitor identification of Moraxella catarrhalis LpxA Insight through crystal structure and computational studies (2017), Int. J. Biol. Macromol., 96, 759-765 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.129 expression in Escherichia coli Moraxella catarrhalis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.129 the residues D69, L70, K71, H117, H120,G138, H139, Q156 from one chain and N193, R199 from the adjacent chain form the active site and play crucial role in substrate positioningand catalysis Moraxella catarrhalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.129 1-benzoyl-2-(1H-indol-3-ylmethyl)-4-(2-oxo-2-(1-pyrrolidinyl)ethyl)-3-piperidinol binding energy score of 4.01. In docked pose, the pyrrolidine ring of the molecule is positioned along H120 of chain A and the indole ring is placed towards chain E. It forms hydrogen bonds with H120 (NE atom) and Q156 (OE1 atom), G139 (N backbone) from chain A and G150 (N backbone) of chain E Moraxella catarrhalis
2.3.1.129 3-((3-(((3,4-dichlorophenoxy)acetyl)amino)-15-pyridin-1-yl)methyl)benzenesulfonyl fluoride binding energy score of 4.4. The dichlorophenoxy-acetyl-amino group occupies a position similar to that of the uridine moiety of the nucleotide substrate. The chlorine atom forms hydrophobic interactions with I147, M165 and V129 of chain E whereas the sulfur group forms pi-sulfur interaction with H120 of chain A Moraxella catarrhalis
2.3.1.129 erythroskyrin binding energy score of ?4.17, spans between two subunits of LpxA interacting with Q68 (OE1 and OE2 atom), L70 (N backbone) and H120 (NE2 atom) of chain A and G196 (backbone O), K200 (NZ atom) of chain E Moraxella catarrhalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.129 84000
-
 gel filtration Moraxella catarrhalis

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.129 Moraxella catarrhalis D5VAW8
-
-
2.3.1.129 Moraxella catarrhalis BBH18 D5VAW8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.129 (R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-galactosamine
-
 Moraxella catarrhalis [acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-galactosamine
-
 ?
2.3.1.129 (R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-galactosamine
-
 Moraxella catarrhalis BBH18 [acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-galactosamine
-
 ?
2.3.1.129 (R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine
-
 Moraxella catarrhalis [acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-glucosamine
-
 ?
2.3.1.129 (R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine
-
 Moraxella catarrhalis BBH18 [acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-glucosamine
-
 ?
2.3.1.129 additional information LpxA displays better binding affinity with UDP-GlcNAc than with UDP-GalNAc. Binding of UDP-GlcNActo LpxA is driven by a very favorable enthalpy change(DeltaH = ?6.03 kcal/mol) with a dissociation constant of 22.62 microM. One monomer of McLpxA binds to one molecule ofUDP-GlcNAc Moraxella catarrhalis ?
-
-
2.3.1.129 additional information LpxA displays better binding affinity with UDP-GlcNAc than with UDP-GalNAc. Binding of UDP-GlcNActo LpxA is driven by a very favorable enthalpy change(DeltaH = ?6.03 kcal/mol) with a dissociation constant of 22.62 microM. One monomer of McLpxA binds to one molecule ofUDP-GlcNAc Moraxella catarrhalis BBH18 ?
-
-

Subunits

EC Number Subunits Comment Organism
2.3.1.129 trimer 3 * 28000, calculated from sequence Moraxella catarrhalis

Synonyms

EC Number Synonyms Comment Organism
2.3.1.129 LpxA
-
 Moraxella catarrhalis