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Literature summary extracted from

  • Li, Q.P.; Wang, C.; Zhang, H.B.; Hu, X.Q.; Li, R.H.; Hua, J.H.
    Designing of a novel dextransucrase efficient in synthesizing oligosaccharides (2017), Int. J. Biol. Macromol., 95, 696-703 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.5 food industry the enzyme synthesizes dextran and oligosaccharides, which act as prebiotics and are popularly used in such industries as food and medicine Leuconostoc mesenteroides
2.4.1.5 medicine the enzyme synthesizes dextran and oligosaccharides, which act as prebiotics and are popularly used in such industries as food and medicine Leuconostoc mesenteroides

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.5 additional information a dextransucrase efficient in synthesizing oligosaccharides is designed. The truncation mutant DSR-S1-DELTAA (residues 1-3087 bp) by deleting the 1494 bp fragment of the C-terminal.The mutant enzyme (MW: 110 kDa) loses activity, when sucrose is used as only substrate. After adding an acceptor, DSR-S1-DELTAA is fully activated but with heavily impaired polysaccharide synthesis ability. The enzyme produces a large amount of oligosaccharides. DSR-S1-DELTAA shows transglycosylation for synthesizing more oligosaccharides of lower degree of polymerization (DP) with different acceptors, and it also improves the selection range of dextransucrase acceptor response to acceptors. The enzyme can be applied in glycodiversifcation studies Leuconostoc mesenteroides

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.5 Leuconostoc mesenteroides Q2I2N5
-
-
2.4.1.5 Leuconostoc mesenteroides 0326 Q2I2N5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.5
-
 Leuconostoc mesenteroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.5 sucrose a dextransucrase efficient in synthesizing oligosaccharides is designed. The truncation mutant DSR-S1-DELTAA (residues 1-3087 bp) by deleting the 1494 bp fragment of the C-terminal.The mutant enzyme (MW: 110 kDa) loses activity, when sucrose is used as only substrate. After adding an acceptor, DSR-S1-DELTAA is fully activated but with heavily impaired polysaccharide synthesis ability. The enzyme produces a large amount of oligosaccharides. DSR-S1-DELTAA shows transglycosylation for synthesizing more oligosaccharides of lower degree of polymerization (DP) with different acceptors, and it also improves the selection range of dextransucrase acceptor response to acceptors. The enzyme can be applied in glycodiversifcation studies Leuconostoc mesenteroides D-fructose + ?
-
 ?
2.4.1.5 sucrose a dextransucrase efficient in synthesizing oligosaccharides is designed. The truncation mutant DSR-S1-DELTAA (residues 1-3087 bp) by deleting the 1494 bp fragment of the C-terminal.The mutant enzyme (MW: 110 kDa) loses activity, when sucrose is used as only substrate. After adding an acceptor, DSR-S1-DELTAA is fully activated but with heavily impaired polysaccharide synthesis ability. The enzyme produces a large amount of oligosaccharides. DSR-S1-DELTAA shows transglycosylation for synthesizing more oligosaccharides of lower degree of polymerization (DP) with different acceptors, and it also improves the selection range of dextransucrase acceptor response to acceptors. The enzyme can be applied in glycodiversifcation studies Leuconostoc mesenteroides 0326 D-fructose + ?
-
 ?
2.4.1.5 sucrose + cellobiose a dextransucrase efficient in synthesizing oligosaccharides is designed. The truncation mutant DSR-S1-DELTAA (residues 1-3087 bp) by deleting the 1494 bp fragment of the C-terminal.The mutant enzyme (MW: 110 kDa) loses activity, when sucrose is used as only substrate. After adding an acceptor, DSR-S1-DELTAA is fully activated but with heavily impaired polysaccharide synthesis ability. The enzyme produces a large amount of oligosaccharides. DSR-S1-DELTAA shows transglycosylation for synthesizing more oligosaccharides of lower degree of polymerization (DP) with different acceptors, and it also improves the selection range of dextransucrase acceptor response to acceptors. The enzyme can be applied in glycodiversifcation studies Leuconostoc mesenteroides D-fructose + ?
-
 ?
2.4.1.5 sucrose + cellobiose a dextransucrase efficient in synthesizing oligosaccharides is designed. The truncation mutant DSR-S1-DELTAA (residues 1-3087 bp) by deleting the 1494 bp fragment of the C-terminal.The mutant enzyme (MW: 110 kDa) loses activity, when sucrose is used as only substrate. After adding an acceptor, DSR-S1-DELTAA is fully activated but with heavily impaired polysaccharide synthesis ability. The enzyme produces a large amount of oligosaccharides. DSR-S1-DELTAA shows transglycosylation for synthesizing more oligosaccharides of lower degree of polymerization (DP) with different acceptors, and it also improves the selection range of dextransucrase acceptor response to acceptors. The enzyme can be applied in glycodiversifcation studies Leuconostoc mesenteroides 0326 D-fructose + ?
-
 ?
2.4.1.5 sucrose + maltose a dextransucrase efficient in synthesizing oligosaccharides is designed. The truncation mutant DSR-S1-DELTAA (residues 1-3087 bp) by deleting the 1494 bp fragment of the C-terminal.The mutant enzyme (MW: 110 kDa) loses activity, when sucrose is used as only substrate. After adding an acceptor, DSR-S1-DELTAA is fully activated but with heavily impaired polysaccharide synthesis ability. The enzyme produces a large amount of oligosaccharides. DSR-S1-DELTAA shows transglycosylation for synthesizing more oligosaccharides of lower degree of polymerization (DP) with different acceptors, and it also improves the selection range of dextransucrase acceptor response to acceptors. The enzyme can be applied in glycodiversifcation studies Leuconostoc mesenteroides D-fructose + ?
-
 ?
2.4.1.5 sucrose + maltose a dextransucrase efficient in synthesizing oligosaccharides is designed. The truncation mutant DSR-S1-DELTAA (residues 1-3087 bp) by deleting the 1494 bp fragment of the C-terminal.The mutant enzyme (MW: 110 kDa) loses activity, when sucrose is used as only substrate. After adding an acceptor, DSR-S1-DELTAA is fully activated but with heavily impaired polysaccharide synthesis ability. The enzyme produces a large amount of oligosaccharides. DSR-S1-DELTAA shows transglycosylation for synthesizing more oligosaccharides of lower degree of polymerization (DP) with different acceptors, and it also improves the selection range of dextransucrase acceptor response to acceptors. The enzyme can be applied in glycodiversifcation studies Leuconostoc mesenteroides 0326 D-fructose + ?
-
 ?
2.4.1.5 sucrose + raffinose a dextransucrase efficient in synthesizing oligosaccharides is designed. The truncation mutant DSR-S1-DELTAA (residues 1-3087 bp) by deleting the 1494 bp fragment of the C-terminal.The mutant enzyme (MW: 110 kDa) loses activity, when sucrose is used as only substrate. After adding an acceptor, DSR-S1-DELTAA is fully activated but with heavily impaired polysaccharide synthesis ability. The enzyme produces a large amount of oligosaccharides. DSR-S1-DELTAA shows transglycosylation for synthesizing more oligosaccharides of lower degree of polymerization (DP) with different acceptors, and it also improves the selection range of dextransucrase acceptor response to acceptors. The enzyme can be applied in glycodiversifcation studies Leuconostoc mesenteroides D-fructose + ?
-
 ?
2.4.1.5 sucrose + raffinose a dextransucrase efficient in synthesizing oligosaccharides is designed. The truncation mutant DSR-S1-DELTAA (residues 1-3087 bp) by deleting the 1494 bp fragment of the C-terminal.The mutant enzyme (MW: 110 kDa) loses activity, when sucrose is used as only substrate. After adding an acceptor, DSR-S1-DELTAA is fully activated but with heavily impaired polysaccharide synthesis ability. The enzyme produces a large amount of oligosaccharides. DSR-S1-DELTAA shows transglycosylation for synthesizing more oligosaccharides of lower degree of polymerization (DP) with different acceptors, and it also improves the selection range of dextransucrase acceptor response to acceptors. The enzyme can be applied in glycodiversifcation studies Leuconostoc mesenteroides 0326 D-fructose + ?
-
 ?
2.4.1.5 sucrose + stachyose tetrahydrate a dextransucrase efficient in synthesizing oligosaccharides is designed. The truncation mutant DSR-S1-DELTAA (residues 1-3087 bp) by deleting the 1494 bp fragment of the C-terminal.The mutant enzyme (MW: 110 kDa) loses activity, when sucrose is used as only substrate. After adding an acceptor, DSR-S1-DELTAA is fully activated but with heavily impaired polysaccharide synthesis ability. The enzyme produces a large amount of oligosaccharides. DSR-S1-DELTAA shows transglycosylation for synthesizing more oligosaccharides of lower degree of polymerization (DP) with different acceptors, and it also improves the selection range of dextransucrase acceptor response to acceptors. The enzyme can be applied in glycodiversifcation studies Leuconostoc mesenteroides D-fructose + ?
-
 ?
2.4.1.5 sucrose + stachyose tetrahydrate a dextransucrase efficient in synthesizing oligosaccharides is designed. The truncation mutant DSR-S1-DELTAA (residues 1-3087 bp) by deleting the 1494 bp fragment of the C-terminal.The mutant enzyme (MW: 110 kDa) loses activity, when sucrose is used as only substrate. After adding an acceptor, DSR-S1-DELTAA is fully activated but with heavily impaired polysaccharide synthesis ability. The enzyme produces a large amount of oligosaccharides. DSR-S1-DELTAA shows transglycosylation for synthesizing more oligosaccharides of lower degree of polymerization (DP) with different acceptors, and it also improves the selection range of dextransucrase acceptor response to acceptors. The enzyme can be applied in glycodiversifcation studies Leuconostoc mesenteroides 0326 D-fructose + ?
-
 ?