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Literature summary extracted from
- L-2-Haloacid dehalogenase from Ancylobacter aquaticus UV5 Sequence determination and structure prediction (2016), Int. J. Biol. Macromol., 83, 216-225 .
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.8.1.2 | 0.47 | - |
Monochloroacetate | pH 9.0, 30°C | Ancylobacter aquaticus |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.2 | 24238 | - |
calculated from sequence | Ancylobacter aquaticus |
| 3.8.1.2 | 25000 | - |
SDS-PAGE | Ancylobacter aquaticus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.8.1.2 | Ancylobacter aquaticus | - |
- |
- |
| 3.8.1.2 | Ancylobacter aquaticus UV5 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.8.1.2 | - |
Ancylobacter aquaticus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.8.1.2 | -20°C, 50 mM Tris-PO4 buffer (pH 9.0), purified protein retains about 90% activity after storage for 3 months | Ancylobacter aquaticus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.8.1.2 | 2,2-dichloropropionate + H2O | 53% of the activity compared to monochloroacetate | Ancylobacter aquaticus | propionate + HCl | - |
? | |
| 3.8.1.2 | 2,2-dichloropropionate + H2O | 53% of the activity compared to monochloroacetate | Ancylobacter aquaticus UV5 | propionate + HCl | - |
? | |
| 3.8.1.2 | L-2-monochloropropionate + H2O | activity with D-2-monochloropropionate is 5% compared to activity with L-2-monochloropropionate | Ancylobacter aquaticus | D-2-hydroxypropionate + HCl | - |
? | |
| 3.8.1.2 | L-2-monochloropropionate + H2O | activity with D-2-monochloropropionate is 5% compared to activity with L-2-monochloropropionate | Ancylobacter aquaticus UV5 | D-2-hydroxypropionate + HCl | - |
? | |
| 3.8.1.2 | monobromoacetate + H2O | 86% of the activity compared to monochloroacetate | Ancylobacter aquaticus | glycolate + HBr | - |
? | |
| 3.8.1.2 | monobromoacetate + H2O | 86% of the activity compared to monochloroacetate | Ancylobacter aquaticus UV5 | glycolate + HBr | - |
? | |
| 3.8.1.2 | monochloroacetate + H2O | - |
Ancylobacter aquaticus | glycolate + HCl | - |
? | |
| 3.8.1.2 | monochloroacetate + H2O | - |
Ancylobacter aquaticus UV5 | glycolate + HCl | - |
? | |
| 3.8.1.2 | monoiodoacetate + H2O | 73% of the activity compared to monochloroacetate | Ancylobacter aquaticus | glycolate + HI | - |
? | |
| 3.8.1.2 | monoiodoacetate + H2O | 73% of the activity compared to monochloroacetate | Ancylobacter aquaticus UV5 | glycolate + HI | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.8.1.2 | ? | x * 25000, SDS-PAGE | Ancylobacter aquaticus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.8.1.2 | L-2-DhlB | - |
Ancylobacter aquaticus |
| 3.8.1.2 | L-2-haloacid dehalogenase | - |
Ancylobacter aquaticus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.2 | 37 | - |
- |
Ancylobacter aquaticus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.2 | 25 | 45 | 25°C: about 45% of maximal activity, 45°C: about 45% of maximal activity | Ancylobacter aquaticus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.2 | 50 | - |
30 min, 50% loss of activity | Ancylobacter aquaticus |
| 3.8.1.2 | 60 | - |
rapid inactivation | Ancylobacter aquaticus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.8.1.2 | 0.08 | - |
Monochloroacetate | pH 9.0, 30°C | Ancylobacter aquaticus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.2 | 9 | - |
- |
Ancylobacter aquaticus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.2 | 5 | 11 | pH 5.0: about 55% of maximal activity, pH 11.0: about 50% of maximal activity | Ancylobacter aquaticus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.2 | 7 | 10 | the enzyme is stable between pH 7-10 retaining 100% of its activity | Ancylobacter aquaticus |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.8.1.2 | Ancylobacter aquaticus | calculated from sequence | - |
5.18 |
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