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Literature summary extracted from

  • Mostafa, F.A.; Abdel Wahab, W.A.; Salah, H.A.; Nawwar, G.A.M.; Esawy, M.A.
    Kinetic and thermodynamic characteristic of Aspergillus awamori EM66 levansucrase (2018), Int. J. Biol. Macromol., 119, 232-239 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.10 NaCl maximum activity at 3.0 M Aspergillus awamori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.10 sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside Aspergillus awamori
-
 D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
 ?
2.4.1.10 sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside Aspergillus awamori EM66
-
 D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.10 Aspergillus awamori
-
-
-
2.4.1.10 Aspergillus awamori EM66
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.10 partial purification by acetone precipitation. Full purification by acetone precipitation and DEAE-Sepharose column chromatography Aspergillus awamori

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.10 2535
-
 crude enzyme, at pH 5.2 and 30°C Aspergillus awamori
2.4.1.10 10590
-
 partially (4.178fold) purified enzyme, at pH 5.2 and 30°C Aspergillus awamori
2.4.1.10 18511
-
 7.3fold purified enzyme, at pH 5.2 and 30°C Aspergillus awamori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.10 sucrose + sucrose
-
 Aspergillus awamori D-glucose + levan
-
 ?
2.4.1.10 sucrose + sucrose
-
 Aspergillus awamori EM66 D-glucose + levan
-
 ?
2.4.1.10 sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
-
 Aspergillus awamori D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
 ?
2.4.1.10 sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
-
 Aspergillus awamori EM66 D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.10 ? x * 44500, SDS-PAGE Aspergillus awamori

Synonyms

EC Number Synonyms Comment Organism
2.4.1.10 fructansucrase
-
 Aspergillus awamori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.10 40
-
-
 Aspergillus awamori

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.10 45 55 he partially purified enzyme form retains about 76% of its original activity after 1 h at 55°C while the purified form retains about 57% after 45 min. The half-life of the partially purified form is 3286, 1150, and 718 min at 45, 50 and 55°C, respectively. The half-life of the fully purified form is 575, 319 and 176 min at 45, 50 and 55°C, respectively Aspergillus awamori