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Literature summary extracted from

  • Chen, S.; Li, Z.; Gu, Z.; Hong, Y.; Cheng, L.; Holler, T.P.; Li, C.
    Leu600 mutations decrease product inhibition of the beta-cyclodextrin glycosyltransferase from Bacillus circulans STB01 (2018), Int. J. Biol. Macromol., 115, 1194-1201 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.19 L600E the mutant shows wild type cyclization activities. The mutation decreases the product inhibition exhibited by beta-cyclodextrin as compared to the wild type enzyme Niallia circulans
2.4.1.19 L600I the mutant shows decreased cyclization activities compared to the wild type enzyme. The mutation decreases the product inhibition exhibited by beta-cyclodextrin as compared to the wild type enzyme Niallia circulans
2.4.1.19 L600R the mutant shows increased cyclization activities compared to the wild type enzyme. The mutation decreases the product inhibition exhibited by beta-cyclodextrin as compared to the wild type enzyme Niallia circulans
2.4.1.19 L600Y the mutant shows wild type cyclization activities. The mutation decreases the product inhibition exhibited by beta-cyclodextrin as compared to the wild type enzyme Niallia circulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.19 beta-cyclodextrin product inhibition, mixed-type inhibition Niallia circulans

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Niallia circulans A0A097CPM3
-
-
2.4.1.19 Niallia circulans STB01 A0A097CPM3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 maltodextrin + glycosyl acceptor 38% conversion Niallia circulans beta-cyclodextrin + gamma-cyclodextrin + alpha-cyclodextrin
-
 ?
2.4.1.19 maltodextrin + glycosyl acceptor 38% conversion Niallia circulans STB01 beta-cyclodextrin + gamma-cyclodextrin + alpha-cyclodextrin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.19 beta-cyclodextrin glycosyltransferase
-
 Niallia circulans
2.4.1.19 CGTase
-
 Niallia circulans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.19 280
-
 maltodextrin mutant enzyme L600Y, at pH 6.5 and 50°C Niallia circulans
2.4.1.19 282
-
 maltodextrin wild type enzyme, at pH 6.5 and 50°C Niallia circulans
2.4.1.19 285
-
 maltodextrin mutant enzyme L600I, at pH 6.5 and 50°C Niallia circulans
2.4.1.19 299
-
 maltodextrin mutant enzyme L600E, at pH 6.5 and 50°C Niallia circulans
2.4.1.19 358
-
 maltodextrin mutant enzyme L600R, at pH 6.5 and 50°C Niallia circulans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.19 0.05
-
 beta-cyclodextrin wild type enzyme, at pH 6.5 and 50°C Niallia circulans
2.4.1.19 0.06
-
 beta-cyclodextrin mutant enzyme L600Y, at pH 6.5 and 50°C Niallia circulans
2.4.1.19 0.19
-
 beta-cyclodextrin mutant enzyme L600E, at pH 6.5 and 50°C Niallia circulans
2.4.1.19 0.28
-
 beta-cyclodextrin mutant enzyme L600I, at pH 6.5 and 50°C Niallia circulans
2.4.1.19 0.5
-
 beta-cyclodextrin mutant enzyme L600R, at pH 6.5 and 50°C Niallia circulans