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Literature summary extracted from
- Glutamic acid at position 152 and serine at position 191 are the key residues required for the metallo-beta-lactamase activity of NDM-7 (2019), Int. J. Antimicrob. Agents, 55, 105824 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.2.6 | gene blaNDM-7, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strains BL21(DE3) and CS109, subcloning in Escherichia coli strain XL-1 Blue. Host Escherichia coli strain CS109 harbouring cloned blaNDM-7 displays a resistant status with over 60fold increased resistance compared to control strain CS109 for all penicillins, cephalosporin and carbapenems tested | Klebsiella pneumoniae |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.5.2.6 | D223A | site-directed mutagenesis, the mutation does not signifcantly affect resistance of the cells against antibiotics | Klebsiella pneumoniae |
| 3.5.2.6 | E152A | site-directed mutagenesis, the mutant shows highly declined resistance to antibiotics compared to wild-type NDM-7 | Klebsiella pneumoniae |
| 3.5.2.6 | E152A/S191A | site-directed mutagenesis, the double mutant shows 2fold reduced antibiotic resistance compared to single mutants, but substantially declined resistance compared to wild-type NDM-7 | Klebsiella pneumoniae |
| 3.5.2.6 | K216A | site-directed mutagenesis, the mutation does not signifcantly affect resistance of the cells against antibiotics | Klebsiella pneumoniae |
| 3.5.2.6 | S191A | site-directed mutagenesis, mutant shows highly declined resistance to antibiotics compared to wild-type NDM-7 | Klebsiella pneumoniae |
| 3.5.2.6 | S213A | site-directed mutagenesis, the mutation does not signifcantly affect resistance of the cells against antibiotics | Klebsiella pneumoniae |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.2.6 | Zn2+ | the active-site residues His120, His122 and His189 are co-ordinated to Zn1 metal ion, whereas Asp124, Cys208 and His250 are co-ordinated to Zn2 metal ion for their catalytic activity. The di-zinc centre of NDM-7 is located at the bottom of the shallow groove and is a part of the binding pocket of the enzyme. The two Zn metal ions present in the active-site cleft take part in the catalytic activity of the NDM-7 via bridging to different active-site residues by forming strong interactions | Klebsiella pneumoniae |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.2.6 | amoxicillin + H2O | Klebsiella pneumoniae | - |
(2R,4S)-2-[(R)-[[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| 3.5.2.6 | amoxicillin + H2O | Klebsiella pneumoniae NDM-7 | - |
(2R,4S)-2-[(R)-[[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| 3.5.2.6 | ampicilin + H2O | Klebsiella pneumoniae | - |
(2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| 3.5.2.6 | carbapenem + H2O | Klebsiella pneumoniae | - |
[(2R)-2,3-dihydro-1H-pyrrol-2-yl]acetic acid | - |
? | |
| 3.5.2.6 | carbapenem + H2O | Klebsiella pneumoniae NDM-7 | - |
[(2R)-2,3-dihydro-1H-pyrrol-2-yl]acetic acid | - |
? | |
| 3.5.2.6 | cephalothin + H2O | Klebsiella pneumoniae | - |
(2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| 3.5.2.6 | doripenem + H2O | Klebsiella pneumoniae | - |
(4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-4-methyl-3-(((3S,5S)-5-[(sulfamoylamino)methyl]pyrrolidin-3-yl)sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
| 3.5.2.6 | imipenem + H2O | Klebsiella pneumoniae | - |
(5R)-3-[[2-(carbonoimidoylamino)ethyl]sulfanyl]-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
| 3.5.2.6 | meropenem + H2O | Klebsiella pneumoniae | - |
(4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
| 3.5.2.6 | additional information | Klebsiella pneumoniae | resistance to antibiotics of recombinant His-tagged wild-type and mutant enzymes expressed in Escherichia coli strain CS109, overview | ? | - |
- |
|
| 3.5.2.6 | additional information | Klebsiella pneumoniae NDM-7 | resistance to antibiotics of recombinant His-tagged wild-type and mutant enzymes expressed in Escherichia coli strain CS109, overview | ? | - |
- |
|
| 3.5.2.6 | penicillin G + H2O | Klebsiella pneumoniae | - |
(2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| 3.5.2.6 | penicillin G + H2O | Klebsiella pneumoniae NDM-7 | - |
(2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.2.6 | Klebsiella pneumoniae | A0A173MZL7 | - |
- |
| 3.5.2.6 | Klebsiella pneumoniae NDM-7 | A0A173MZL7 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.2.6 | recombinant active N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography to homogeneity | Klebsiella pneumoniae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.2.6 | amoxicillin + H2O | - |
Klebsiella pneumoniae | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| 3.5.2.6 | amoxicillin + H2O | - |
Klebsiella pneumoniae NDM-7 | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| 3.5.2.6 | ampicilin + H2O | - |
Klebsiella pneumoniae | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| 3.5.2.6 | carbapenem + H2O | - |
Klebsiella pneumoniae | [(2R)-2,3-dihydro-1H-pyrrol-2-yl]acetic acid | - |
? | |
| 3.5.2.6 | carbapenem + H2O | - |
Klebsiella pneumoniae NDM-7 | [(2R)-2,3-dihydro-1H-pyrrol-2-yl]acetic acid | - |
? | |
| 3.5.2.6 | cephalothin + H2O | - |
Klebsiella pneumoniae | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| 3.5.2.6 | doripenem + H2O | - |
Klebsiella pneumoniae | (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-4-methyl-3-(((3S,5S)-5-[(sulfamoylamino)methyl]pyrrolidin-3-yl)sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
| 3.5.2.6 | imipenem + H2O | - |
Klebsiella pneumoniae | (5R)-3-[[2-(carbonoimidoylamino)ethyl]sulfanyl]-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
| 3.5.2.6 | meropenem + H2O | - |
Klebsiella pneumoniae | (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
| 3.5.2.6 | additional information | resistance to antibiotics of recombinant His-tagged wild-type and mutant enzymes expressed in Escherichia coli strain CS109, overview | Klebsiella pneumoniae | ? | - |
- |
|
| 3.5.2.6 | additional information | purified enzyme NDM-7 exhibits broad-spectrum enzyme activities with low Km and high kcat values hydrolysing all beta-lactam substrates tested | Klebsiella pneumoniae | ? | - |
- |
|
| 3.5.2.6 | additional information | resistance to antibiotics of recombinant His-tagged wild-type and mutant enzymes expressed in Escherichia coli strain CS109, overview | Klebsiella pneumoniae NDM-7 | ? | - |
- |
|
| 3.5.2.6 | additional information | purified enzyme NDM-7 exhibits broad-spectrum enzyme activities with low Km and high kcat values hydrolysing all beta-lactam substrates tested | Klebsiella pneumoniae NDM-7 | ? | - |
- |
|
| 3.5.2.6 | penicillin G + H2O | - |
Klebsiella pneumoniae | (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| 3.5.2.6 | penicillin G + H2O | - |
Klebsiella pneumoniae NDM-7 | (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.2.6 | blaNDM-7 | - |
Klebsiella pneumoniae |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.5.2.6 | additional information | Glu152 and Ser191 are the key residues required for the metallo-beta-lactamase activity of strain NDM-7. Three-dimensional structure homology modelling of wild-type BlaNDM-7 and mutant enzymes | Klebsiella pneumoniae |
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