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Literature summary extracted from

  • Saravanan, T.; Reif, M.; Yi, D.; Lorilliere, M.; Charmantray, F.; Hecquet, L.; Fessner, W.
    Engineering a thermostable transketolase for arylated substrates (2017), Green Chem., 19, 481-489 .
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
2.2.1.1 D470T the mutant shows slightly increased activity with benzaldehyde compared to the wild type enzyme Geobacillus stearothermophilus
2.2.1.1 L382D/D470S the mutant shows strongly increased activity with benzaldehyde compared to the wild type enzyme Geobacillus stearothermophilus
2.2.1.1 L382E/D470T the mutant shows increased activity with benzaldehyde compared to the wild type enzyme Geobacillus stearothermophilus
2.2.1.1 L382N/D470S the mutant shows strongly increased activity with benzaldehyde compared to the wild type enzyme Geobacillus stearothermophilus
2.2.1.1 L382N/D470T the mutant shows increased activity with benzaldehyde compared to the wild type enzyme Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.1 fructose 6-phosphate + D-glyceraldehyde 3-phosphate Geobacillus stearothermophilus
-
D-erythrose 4-phosphate + D-xylulose 5-phosphate
-
r
2.2.1.1 sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate Geobacillus stearothermophilus
-
D-ribose 5-phosphate + D-xylulose 5-phosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.1 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.1 Ni-NTA column chromatography Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.1 2-phenylethanal + hydroxypyruvate
-
Geobacillus stearothermophilus (S)-1,3-dihydroxy-4-phenylbutan-2-one + CO2
-
ir
2.2.1.1 3-phenylpropanal + hydroxypyruvate
-
Geobacillus stearothermophilus (S)-1,3-dihydroxy-5-phenylpentan-2-one + CO2
-
ir
2.2.1.1 benzaldehyde + hydroxypyruvate the wild type enzyme shows very little activity against benzaldehyde Geobacillus stearothermophilus (R)-1,3-dihydroxy-1-phenylpropan-2-one + CO2
-
ir
2.2.1.1 fructose 6-phosphate + D-glyceraldehyde 3-phosphate
-
Geobacillus stearothermophilus D-erythrose 4-phosphate + D-xylulose 5-phosphate
-
r
2.2.1.1 sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
Geobacillus stearothermophilus D-ribose 5-phosphate + D-xylulose 5-phosphate
-
r

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.1 thiamine diphosphate
-
Geobacillus stearothermophilus