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Literature summary extracted from

  • Subrizi, F.; Cardenas-Fernandez, M.; Lye, G.; Ward, J.; Dalby, P.; Sheppard, T.; Hailes, H.
    Transketolase catalysed upgrading of L-arabinose The one-step stereoselective synthesis of L-gluco-heptulose (2016), Green Chem., 18, 3158-3165 .
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
2.2.1.1 H461Y the mutant shows about 1.2fold increased activity with L-arabinose compared to the wild type enzyme Escherichia coli
2.2.1.1 R358I the mutant shows about 1.2fold increased activity with L-arabinose compared to the wild type enzyme Escherichia coli
2.2.1.1 R358P the mutant shows about 1.4fold increased activity with L-arabinose compared to the wild type enzyme Escherichia coli
2.2.1.1 R358S the mutant shows about 1.4fold increased activity with L-arabinose compared to the wild type enzyme Escherichia coli
2.2.1.1 R520P the mutant shows about 1.5fold increased activity with L-arabinose compared to the wild type enzyme Escherichia coli
2.2.1.1 R520Y the mutant shows about 2fold increased activity with L-arabinose compared to the wild type enzyme Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.1 Mg2+ 6 mM used in assay conditions Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.1 sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate Escherichia coli
-
 D-ribose 5-phosphate + D-xylulose 5-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.1 Escherichia coli P27302
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.1 L-arabinose + lithium beta-hydroxypyruvate 48% conversion after 24 h Escherichia coli L-gluco-heptulose + CO2 + Li+
-
 ?
2.2.1.1 sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 Escherichia coli D-ribose 5-phosphate + D-xylulose 5-phosphate
-
 r

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.1 thiamine diphosphate
-
 Escherichia coli