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Literature summary extracted from

  • Toopaang, W.; Phonghanpot, S.; Punya, J.; Panyasiri, C.; Klamchao, K.; Wasuwan, R.; Srisuksam, C.; Sangsrakru, D.; Sonthirod, C.; Tangphatsornruang, S.; Tanticharoen, M.; Amnuaykanjanasin, A.
    Targeted disruption of the polyketide synthase gene pks15 affects virulence against insects and phagocytic survival in the fungus Beauveria bassiana (2017), Fungal Biol., 121, 664-675 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.261 gene pks15, genetic structure, recombinant expression in Beauveria bassiana strain BCC2660 mutant DELTA pks15 Beauveria bassiana

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.261 additional information the DELTApks15 mutant is disrupted by a single integration of the transformation cassette at the pks15 locus. Disruption vector construction and Agrobacterium tumefaciens strain EHA105-mediated transformation of Beauveria bassiana strain BCC2660, pks15 gene expression analysis in the disruptant mutant and in wild-type. Mutant DELTApks15 phenotype, overview Beauveria bassiana

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.261 Beauveria bassiana A0A1W5T7B8
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2.3.1.261 Beauveria bassiana BCC2660 A0A1W5T7B8
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Source Tissue

EC Number Source Tissue Comment Organism Textmining

Synonyms

EC Number Synonyms Comment Organism
2.3.1.261 Pks15
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 Beauveria bassiana
2.3.1.261 type I PKS
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 Beauveria bassiana

General Information

EC Number General Information Comment Organism
2.3.1.261 malfunction the Dpks15 mutant has a slight reduction in radial growth and produces fewer spores compared to wild-type. Insect bioassays indicate the DELTApks15 mutant to be significantly reduced in virulence against beet armyworms compared to wild-type, which can be partially accounted for by its markedly decreased ability to survive phagocytosis. Total haemocyte count decreases sharply by 50fold from days 1-3 post-inoculation in insects infected with wild-type, compared to a 5fold decrease in the DELTApks15 mutant. The mutant also produces 3fold fewer hemolymph hyphal bodies than the wild-type. In co-culture studies with amoebae that have phagocytic ability similar to that of insect haemocytes, the mortality rate of amoebae engulfing DELTApks15 decreases by 72% during 48 h, and DELTApks15 CFU decrease by 83% compared to co-culture with the wild-type. Thus, the DELTApks15 mutant has a reduced ability to cope with phagocytosis and highly reduced virulence in an insect host Beauveria bassiana