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Literature summary extracted from

  • Ruszkowski, M.; Sekula, B.; Ruszkowska, A.; Dauter, Z.
    Chloroplastic serine hydroxymethyltransferase from Medicago truncatula A structural characterization (2018), Front. Plant Sci., 9, 584 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 expressed in Escherichia coli BL21 gold cells Medicago truncatula

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.2.1 apoenzyme,hanging drop vapor diffusion method, using 55% tacsimate pH 7.0. Enzyme bound to reaction intermediates, hanging drop vapor diffusion method, using 75 mM MES pH 6.5, 19%(w/v) polyethylene glycol 3350 and 150 mMammonium acetate Medicago truncatula

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.1 chloroplast
-
 Medicago truncatula 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 150000
-
 gel filtration Medicago truncatula
2.1.2.1 197000
-
 theoretical molecular mass of the tetramer Medicago truncatula

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Medicago truncatula
-
 tetrahydrofolate + L-serine
-
 r
2.1.2.1 tetrahydrofolate + L-serine Medicago truncatula
-
 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Medicago truncatula G7ILW0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1 HisTrap resin column chromatography and Superdex S200 gel filtration Medicago truncatula

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
 Medicago truncatula tetrahydrofolate + L-serine
-
 r
2.1.2.1 tetrahydrofolate + L-serine
-
 Medicago truncatula 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.2.1 tetramer
-
 Medicago truncatula

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 serine hydroxymethyltransferase
-
 Medicago truncatula
2.1.2.1 SHMT3
-
 Medicago truncatula

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate
-
 Medicago truncatula