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Literature summary extracted from

  • Li, J.; Li, C.; Gou, J.; Wang, X.; Fan, R.; Zhang, Y.
    An alternative pathway for formononetin biosynthesis in Pueraria lobata (2016), Front. Plant Sci., 7, 861 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.46 expressed in Escherichia coli BL21(DE3) cells Pueraria montana var. lobata

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.46 Pueraria montana var. lobata A0A1L1Z441
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.46 Ni-NTA column chromatography Pueraria montana var. lobata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.46 leaf
-
 Pueraria montana var. lobata
-
2.1.1.46 root highest expression Pueraria montana var. lobata
-
2.1.1.46 stem
-
 Pueraria montana var. lobata
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.46 additional information the enzyme is not able to methylate 2,7,4'-trihydroxyisoflavanone, biochanin A, formononetin, apigenin, luteolin, liquirtitigenin, kaempferol, quercetin, isoliquiritigenin, and caffeic acid Pueraria montana var. lobata ?
-
-
2.1.1.46 S-adenosyl-L-methionine + daidzein 48.8% activity compared to genistein Pueraria montana var. lobata S-adenosyl-L-homocysteine + formononetin
-
 ?
2.1.1.46 S-adenosyl-L-methionine + genistein 100% activity Pueraria montana var. lobata S-adenosyl-L-homocysteine + biochanin A
-
 ?
2.1.1.46 S-adenosyl-L-methionine + isoformononetin 5.2% activity compared to genistein Pueraria montana var. lobata S-adenosyl-L-homocysteine + 4',7-dimethoxyisoflavone
-
 ?
2.1.1.46 S-adenosyl-L-methionine + prunetin 6.2% activity compared to genistein Pueraria montana var. lobata S-adenosyl-L-homocysteine + 4',7-dimethoxy-5-hydroxyisoflavone
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.46 I4'OMT
-
 Pueraria montana var. lobata
2.1.1.46 isoflavone-specific 4'-O-methyltransferase
-
 Pueraria montana var. lobata
2.1.1.46 OMT9
-
 Pueraria montana var. lobata

Expression

EC Number Organism Comment Expression
2.1.1.46 Pueraria montana var. lobata enzyme expression is increased about 3fold in the presence of methyl jasmonate up