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Literature summary extracted from

  • Li, J.; Li, C.; Gou, J.; Zhang, Y.
    Molecular cloning and functional characterization of a novel isoflavone 3'-O-methyltransferase from Pueraria lobata (2016), Front. Plant Sci., 7, 793 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.B121 expression in Escherichia coli and Saccharomyces cerevisiae Pueraria montana var. lobata

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.B121 Pueraria montana var. lobata A0A1L1Z440
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.B121 leaf very poor expression Pueraria montana var. lobata
-
2.1.1.B121 root highest expression level Pueraria montana var. lobata
-
2.1.1.B121 stem very poor expression Pueraria montana var. lobata
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.B121 S-adenosyl-L-methionine + 3'-hydroxydaidzein
-
 Pueraria montana var. lobata S-adenosyl-L-homocysteine + 3'-methoxydaidzein
-
 ?
2.1.1.B121 S-adenosyl-L-methionine + 3'-hydroxypuerarin
-
 Pueraria montana var. lobata S-adenosyl-L-homocysteine + 3'-methoxypuerarin
-
 ?
2.1.1.B121 S-adenosyl-L-methionine + luteolin
-
 Pueraria montana var. lobata S-adenosyl-L-homocysteine + chrysoeriol
-
 ?
2.1.1.B121 S-adenosyl-L-methionine + quercetin
-
 Pueraria montana var. lobata S-adenosyl-L-homocysteine + isorhamnetin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.B121 ? x * 45000, SDS-PAGE, recombinant protein Pueraria montana var. lobata

Synonyms

EC Number Synonyms Comment Organism
2.1.1.B121 OMT4
-
 Pueraria montana var. lobata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.B121 37
-
 assay at Pueraria montana var. lobata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.B121 8
-
 assay at Pueraria montana var. lobata

Expression

EC Number Organism Comment Expression
2.1.1.B121 Pueraria montana var. lobata application of methyl jasmonate significantly increases transcript levels of OMT4 and biosynthesis of 3'-methoxydaidzein up