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Literature summary extracted from

  • Allegretta, G.; Maurer, C.; Eberhard, J.; Maura, D.; Hartmann, R.; Rahme, L.; Empting, M.
    In-depth profiling of MvfR-regulated small molecules in Pseudomonas aeruginosa after Quorum Sensing inhibitor treatment (2017), Front. Microbiol., 8, 924 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.B38 additional information Pseudomonas aeruginosa strain PA14 isogenic transposon pqsC mutant Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.B38 (furan-3-yl)(2-nitrophenyl)methanol PqsBC inhibitor reduces the production of the MvfR natural ligands in wild-type strain PA14 down to 34% at its highest concentration Pseudomonas aeruginosa
2.3.1.B38 ethyl 3-benzyl-1-[2-(4-fluoroanilino)-2-oxoethyl]-2,4-dioxo-1,3,4,5,6,8-hexahydropyrido[4',3':4,5]thieno[2,3-d]pyrimidine-7(2H)-carboxylate PqsBC inhibitor reduces the production of the MvfR natural ligands in wild-type strain PA14 down to 35% at its highest concentration Pseudomonas aeruginosa
2.3.1.B38 additional information Pseudomonas aeruginosa is a Gram-negative bacterium, which causes opportunistic infections in immuno-compromised individuals. Due to its multiple resistances toward antibiotics, the development of new drugs is required. Interfering with quorum sensing (QS), a cell-to-cell communication system, has shown to be highly efficient in reducing Pseudomonas aeruginosa pathogenicity. One of its QS systems employs Pseudomonas quinolone signal (PQS) and 4-hydroxy-2-heptylquinoline (HHQ) as signal molecules. Both activate the transcriptional regulator MvfR (Multiple Virulence Factor Regulator), also called PqsR, driving the production of QS molecules as well as toxins and biofilm formation. Effects of QS inhibitors (QSIs), such as MvfR antagonists and PqsBC inhibitors, on the biosynthesis of the MvfR-regulated small molecules 2'-aminoacetophenone (2-AA), dihydroxyquinoline (DHQ), 4-hydroxy-2-heptylquinoline (HHQ), Pseudomonas quinolone signal (PQS), and 4-hydroxy-2-heptylquinoline-N-oxide (HQNO). The employed synthetic MvfR antagonist fully inhibits pqs small molecule formation showing expected sigmoidal dose-response curves for 2-AA, HQNO, HHQ, and PQS Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.B38 octanoyl-CoA + (2-aminobenzoyl)acetate Pseudomonas aeruginosa
-
 2-heptyl-4-quinolone + CoA + CO2 + H2O
-
 ?
2.3.1.B38 octanoyl-CoA + (2-aminobenzoyl)acetate Pseudomonas aeruginosa ATCC 15692
-
 2-heptyl-4-quinolone + CoA + CO2 + H2O
-
 ?
2.3.1.B38 octanoyl-CoA + (2-aminobenzoyl)acetate Pseudomonas aeruginosa 1C
-
 2-heptyl-4-quinolone + CoA + CO2 + H2O
-
 ?
2.3.1.B38 octanoyl-CoA + (2-aminobenzoyl)acetate Pseudomonas aeruginosa PRS 101
-
 2-heptyl-4-quinolone + CoA + CO2 + H2O
-
 ?
2.3.1.B38 octanoyl-CoA + (2-aminobenzoyl)acetate Pseudomonas aeruginosa DSM 22644
-
 2-heptyl-4-quinolone + CoA + CO2 + H2O
-
 ?
2.3.1.B38 octanoyl-CoA + (2-aminobenzoyl)acetate Pseudomonas aeruginosa CIP 104116
-
 2-heptyl-4-quinolone + CoA + CO2 + H2O
-
 ?
2.3.1.B38 octanoyl-CoA + (2-aminobenzoyl)acetate Pseudomonas aeruginosa LMG 12228
-
 2-heptyl-4-quinolone + CoA + CO2 + H2O
-
 ?
2.3.1.B38 octanoyl-CoA + (2-aminobenzoyl)acetate Pseudomonas aeruginosa JCM 14847
-
 2-heptyl-4-quinolone + CoA + CO2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.B38 Pseudomonas aeruginosa Q9I4X1 AND Q9I4X2 2-heptyl-4(1H)-quinolone synthase subunits PqsC and PqsB
-
2.3.1.B38 Pseudomonas aeruginosa 1C Q9I4X1 AND Q9I4X2 2-heptyl-4(1H)-quinolone synthase subunits PqsC and PqsB
-
2.3.1.B38 Pseudomonas aeruginosa ATCC 15692 Q9I4X1 AND Q9I4X2 2-heptyl-4(1H)-quinolone synthase subunits PqsC and PqsB
-
2.3.1.B38 Pseudomonas aeruginosa CIP 104116 Q9I4X1 AND Q9I4X2 2-heptyl-4(1H)-quinolone synthase subunits PqsC and PqsB
-
2.3.1.B38 Pseudomonas aeruginosa DSM 22644 Q9I4X1 AND Q9I4X2 2-heptyl-4(1H)-quinolone synthase subunits PqsC and PqsB
-
2.3.1.B38 Pseudomonas aeruginosa JCM 14847 Q9I4X1 AND Q9I4X2 2-heptyl-4(1H)-quinolone synthase subunits PqsC and PqsB
-
2.3.1.B38 Pseudomonas aeruginosa LMG 12228 Q9I4X1 AND Q9I4X2 2-heptyl-4(1H)-quinolone synthase subunits PqsC and PqsB
-
2.3.1.B38 Pseudomonas aeruginosa PRS 101 Q9I4X1 AND Q9I4X2 2-heptyl-4(1H)-quinolone synthase subunits PqsC and PqsB
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.B38 octanoyl-CoA + (2-aminobenzoyl)acetate
-
 Pseudomonas aeruginosa 2-heptyl-4-quinolone + CoA + CO2 + H2O
-
 ?
2.3.1.B38 octanoyl-CoA + (2-aminobenzoyl)acetate
-
 Pseudomonas aeruginosa ATCC 15692 2-heptyl-4-quinolone + CoA + CO2 + H2O
-
 ?
2.3.1.B38 octanoyl-CoA + (2-aminobenzoyl)acetate
-
 Pseudomonas aeruginosa 1C 2-heptyl-4-quinolone + CoA + CO2 + H2O
-
 ?
2.3.1.B38 octanoyl-CoA + (2-aminobenzoyl)acetate
-
 Pseudomonas aeruginosa PRS 101 2-heptyl-4-quinolone + CoA + CO2 + H2O
-
 ?
2.3.1.B38 octanoyl-CoA + (2-aminobenzoyl)acetate
-
 Pseudomonas aeruginosa DSM 22644 2-heptyl-4-quinolone + CoA + CO2 + H2O
-
 ?
2.3.1.B38 octanoyl-CoA + (2-aminobenzoyl)acetate
-
 Pseudomonas aeruginosa CIP 104116 2-heptyl-4-quinolone + CoA + CO2 + H2O
-
 ?
2.3.1.B38 octanoyl-CoA + (2-aminobenzoyl)acetate
-
 Pseudomonas aeruginosa LMG 12228 2-heptyl-4-quinolone + CoA + CO2 + H2O
-
 ?
2.3.1.B38 octanoyl-CoA + (2-aminobenzoyl)acetate
-
 Pseudomonas aeruginosa JCM 14847 2-heptyl-4-quinolone + CoA + CO2 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.B38 2-heptyl-4(1H)-quinolone synthase
-
 Pseudomonas aeruginosa
2.3.1.B38 PqsB
-
 Pseudomonas aeruginosa
2.3.1.B38 PqsBC
-
 Pseudomonas aeruginosa
2.3.1.B38 PqsC
-
 Pseudomonas aeruginosa

General Information

EC Number General Information Comment Organism
2.3.1.B38 malfunction effects of QS inhibitors (QSIs), such as MvfR antagonists and PqsBC inhibitors, on the biosynthesis of the MvfR-regulated small molecules 2'-aminoacetophenone (2-AA), dihydroxyquinoline (DHQ), 4-hydroxy-2-heptylquinoline (HHQ), Pseudomonas quinolone signal (PQS), and 4-hydroxy-2-heptylquinoline-N-oxide (HQNO) Pseudomonas aeruginosa
2.3.1.B38 metabolism the 2-heptyl-4(1H)-quinolone synthase is involved in the biosynthetic pathway of MvfR-related small molecules. In-depth profiling of MvfR-regulated small molecules in Pseudomonas aeruginosa after quorum sensing inhibitor treatment Pseudomonas aeruginosa