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Literature summary extracted from

  • Park, I.; Park, M.; Yoon, N.; Cha, J.
    Comparison of the structural properties and nutritional fraction of corn starch treated with thermophilic GH13 and GH57 alpha-glucan branching enzymes (2019), Foods, 8, 452 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.18 expressed in Escherichia coli BL21(DE3) cells Pyrococcus horikoshii
2.4.1.18 expressed in Escherichia coli BL21(DE3) cells Caldicellulosiruptor bescii

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.18 Caldicellulosiruptor bescii B9MPC3
-
-
2.4.1.18 Caldicellulosiruptor bescii ATCC BAA-1888 B9MPC3
-
-
2.4.1.18 Pyrococcus horikoshii O50094
-
-
2.4.1.18 Pyrococcus horikoshii OT-3 O50094
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.18 Ni-NTA agarose column chromatography Pyrococcus horikoshii
2.4.1.18 Ni-NTA agarose column chromatography Caldicellulosiruptor bescii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.18 amylopectin
-
 Pyrococcus horikoshii amylopectin containing alpha-1,6-glucosidic linkages
-
 ?
2.4.1.18 amylopectin
-
 Caldicellulosiruptor bescii amylopectin containing alpha-1,6-glucosidic linkages
-
 ?
2.4.1.18 amylopectin
-
 Caldicellulosiruptor bescii ATCC BAA-1888 amylopectin containing alpha-1,6-glucosidic linkages
-
 ?
2.4.1.18 amylopectin
-
 Pyrococcus horikoshii OT-3 amylopectin containing alpha-1,6-glucosidic linkages
-
 ?
2.4.1.18 amylose
-
 Caldicellulosiruptor bescii amylose containing alpha-1,6-glucosidic linkages
-
 ?
2.4.1.18 amylose the enzyme shows limited activity for substrates containing only alpha-1,4 linkages such as amylose Pyrococcus horikoshii amylose containing alpha-1,6-glucosidic linkages
-
 ?
2.4.1.18 amylose
-
 Caldicellulosiruptor bescii ATCC BAA-1888 amylose containing alpha-1,6-glucosidic linkages
-
 ?
2.4.1.18 amylose the enzyme shows limited activity for substrates containing only alpha-1,4 linkages such as amylose Pyrococcus horikoshii OT-3 amylose containing alpha-1,6-glucosidic linkages
-
 ?
2.4.1.18 corn starch
-
 Pyrococcus horikoshii corn starch containing alpha-1,6-glucosidic linkages
-
 ?
2.4.1.18 corn starch
-
 Caldicellulosiruptor bescii corn starch containing alpha-1,6-glucosidic linkages
-
 ?
2.4.1.18 corn starch
-
 Caldicellulosiruptor bescii ATCC BAA-1888 corn starch containing alpha-1,6-glucosidic linkages
-
 ?
2.4.1.18 corn starch
-
 Pyrococcus horikoshii OT-3 corn starch containing alpha-1,6-glucosidic linkages
-
 ?
2.4.1.18 high-amylose corn starch
-
 Pyrococcus horikoshii high-amylose corn starch containing alpha-1,6-glucosidic linkages
-
 ?
2.4.1.18 high-amylose corn starch
-
 Caldicellulosiruptor bescii high-amylose corn starch containing alpha-1,6-glucosidic linkages
-
 ?
2.4.1.18 high-amylose corn starch
-
 Caldicellulosiruptor bescii ATCC BAA-1888 high-amylose corn starch containing alpha-1,6-glucosidic linkages
-
 ?
2.4.1.18 high-amylose corn starch
-
 Pyrococcus horikoshii OT-3 high-amylose corn starch containing alpha-1,6-glucosidic linkages
-
 ?
2.4.1.18 additional information the branching activity of the enzyme contains both alpha-1,4 and alpha-1,6 linkages Caldicellulosiruptor bescii ?
-
-
2.4.1.18 additional information the branching activity of the enzyme contains both alpha-1,4 and alpha-1,6 linkages Caldicellulosiruptor bescii ATCC BAA-1888 ?
-
-

Subunits

EC Number Subunits Comment Organism
2.4.1.18 ? x * 66000, SDS-PAGE Pyrococcus horikoshii
2.4.1.18 ? x * 70000, SDS-PAGE Caldicellulosiruptor bescii

Synonyms

EC Number Synonyms Comment Organism
2.4.1.18 amylo-(1,4->1,6)-transglycosylase
-
 Pyrococcus horikoshii
2.4.1.18 amylo-(1,4->1,6)-transglycosylase
-
 Caldicellulosiruptor bescii
2.4.1.18 GBE
-
 Pyrococcus horikoshii
2.4.1.18 GBE
-
 Caldicellulosiruptor bescii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.18 60
-
-
 Pyrococcus horikoshii
2.4.1.18 60
-
-
 Caldicellulosiruptor bescii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.18 6
-
-
 Pyrococcus horikoshii
2.4.1.18 6
-
-
 Caldicellulosiruptor bescii