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Literature summary extracted from

  • Sonnendecker, C.; Zimmermann, W.
    Domain shuffling of cyclodextrin glucanotransferases for tailored product specificity and thermal stability (2019), FEBS Open Bio, 9, 384-395 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.19 expressed in Escherichia coli BL21(DE3) cells Geobacillus stearothermophilus
2.4.1.19 expressed in Escherichia coli BL21(DE3) cells Bacillus sp. G-825-6

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.19 Mg2+ 10 mM used in assay conditions Geobacillus stearothermophilus
2.4.1.19 Mg2+ 10 mM used in assay conditions Bacillus sp. G-825-6

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Bacillus sp. G-825-6
-
-
-
2.4.1.19 Geobacillus stearothermophilus
-
-
-
2.4.1.19 Geobacillus stearothermophilus ET1/NO2
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus sp. G-825-6 gamma-cyclodextrin
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor
-
 Geobacillus stearothermophilus alpha-cyclodextrin + beta-cyclodextrin
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor
-
 Geobacillus stearothermophilus ET1/NO2 alpha-cyclodextrin + beta-cyclodextrin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.19 CGTase
-
 Geobacillus stearothermophilus
2.4.1.19 CGTase
-
 Bacillus sp. G-825-6
2.4.1.19 cyclodextrin glucanotransferase
-
 Geobacillus stearothermophilus
2.4.1.19 cyclodextrin glucanotransferase
-
 Bacillus sp. G-825-6

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.19 50 60 most active Bacillus sp. G-825-6
2.4.1.19 70 80 most active Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.19 71
-
 melting temperature Bacillus sp. G-825-6
2.4.1.19 88
-
 melting temperature Geobacillus stearothermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.19 5 7 most active Geobacillus stearothermophilus
2.4.1.19 8 10 most active Bacillus sp. G-825-6