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Literature summary extracted from

  • Du, X.; Hu, J.; Zhang, Q.; Liu, Q.; Xiang, X.; Dong, J.; Lou, B.; He, S.; Gu, X.; Cao, Y.; Li, Y.; Ding, T.
    A novel assay for measuring recombinant human lysophosphatidylcholine acyltransferase 3 activity (2019), FEBS open bio, 9, 1734-1743 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.23 medicine LPCAT3 may have potential as a therapeutic target for diseases, including atherosclerosis, non-alcoholic steatohepatitis, and carcinoma Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.23 gene LPCAT3, recombinant expression of C-terminally Twin-Strep-tagged enzyme in Spodoptera frugiperda Sf9 cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.23 TSI-10 an LPCAT3 inhibitor Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.23 additional information
-
 additional information Km of NBD-lyso-PC and arachidonoyl CoA by the model of Bi-Bi reaction under optimal conditions. The theoretical Km values calculated by Bi-Bi kinetic equations are more accurate than that calculated by the Michaelis-Menten equation because the apparent Km of one substrate calculated by the Michaelis-Menten equation may be influenced by the concentration of another, whereas Bi-Bi kinetic equations could overcome this disadvantage. From the Lineweaver-Burk plots, a sequential kinetic mechanism can be proposed, but only a sequential Bi-Bi kinetic mechanism can be infered Homo sapiens
2.3.1.23 0.011
-
 Arachidonoyl-CoA pH 6.0, 30°C, recombinant enzyme Homo sapiens
2.3.1.23 0.2668
-
 NBD-labelled lysophosphatidylcholine pH 6.0, 30°C, recombinant enzyme Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.23 membrane
-
 Homo sapiens 16020
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.23 Homo sapiens Q6P1A2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.23 recombinant Twin-Strep-tagged enzyme from Sf9 insect cells by membrane preparation, affinity chromatography, and gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.23 arachidonoyl-CoA + NBD-labelled lysophosphatidylcholine
-
 Homo sapiens CoA + NBD-2-arachidonoyl-phosphatidylcholine
-
-

Synonyms

EC Number Synonyms Comment Organism
2.3.1.23 LPCAT3
-
 Homo sapiens
2.3.1.23 LPLAT5
-
 Homo sapiens
2.3.1.23 lysophosphatidylcholine acyltransferase 3
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.23 30
-
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.23 6
-
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.23 0.174
-
 pH 6.0, 30°C, recombinant enzyme Homo sapiens TSI-10

General Information

EC Number General Information Comment Organism
2.3.1.23 physiological function lysophosphatidylcholine acyltransferase 3 (LPCAT3) is an important enzyme in phospholipid remodeling, a process that influences the biophysical properties of cell membranes and thus cell function. LPCAT3 is involved in several diseases, including atherosclerosis, non-alcoholic steatohepatitis, and carcinoma Homo sapiens