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Literature summary extracted from

  • Ohashi, H.; Ohashi, T.; Misaki, R.; Fujiyama, K.
    Arabidopsis thaliana alpha1,2-l-fucosyltransferase catalyzes the transfer of l-galactose to xyloglucan oligosaccharides (2019), FEBS Lett., 593, 187-194 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.69 expression in Schizosaccharomyces pombe Arabidopsis thaliana
2.4.1.344 expression in Schizosaccharomyces pombe Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.69 Mn2+ 10 mM, 16% decrease in activity Arabidopsis thaliana
2.4.1.69 additional information the L-galactosyltransferase activity of Fut1 is found in the absence of divalent cations and in the presence of EDTA Arabidopsis thaliana
2.4.1.344 Mn2+ 10 mM, 16% inhibition Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.344 membrane recombinant protein Arabidopsis thaliana 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.69 Ca2+ 5 mM, 8-13% activation Arabidopsis thaliana
2.4.1.69 Mg2+ 5 mM, 8-13% activation Arabidopsis thaliana
2.4.1.69 additional information the L-galactosyltransferase activity of Fut1 is found in the absence of divalent cations and in the presence of EDTA Arabidopsis thaliana
2.4.1.344 Ca2+ 5 mM, 8-13% stimulation Arabidopsis thaliana
2.4.1.344 Mg2+ 5 mM, 8-13% stimulation Arabidopsis thaliana
2.4.1.344 additional information no divalent cation required Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.69 Arabidopsis thaliana Q9SWH5
-
-
2.4.1.344 Arabidopsis thaliana Q9SWH5 isoform FUT1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.69 GDP-L-Fuc + XLLGXLLG-2-aminopyridine acceptor xyloglucan oligosaccharide carrying two repeated XLLG units plus 2-aminopyridine Arabidopsis thaliana GDP + alpha-L-fucosyl-XLLGXLLG-2-aminopyridine
-
 ?
2.4.1.69 GDP-L-Gal + xyloglucan oligosaccharide
-
 Arabidopsis thaliana GDP + L-galactosyl-xyloglucan oligosaccharide
-
 ?
2.4.1.69 additional information recombinant FUT1 catalyzes L-Gal transfer to xyloglucan oligosaccharides with an initial velocity of L-Gal transfer 3.1 times lower than that of L-Fuc transfer. No acceptor: mono-D-galactosylated xyloglucan oligosaccharide XXLG-2-aminopyridine, XLLG-2-aminopyridine Arabidopsis thaliana ?
-
-
2.4.1.344 GDP-L-Fuc + xyloglucan acceptor is nonlabeled XLLG oligosaccharide Arabidopsis thaliana GDP + alpha-L-Fuc-xyloglucan
-
 ?
2.4.1.344 GDP-L-Gal + xyloglucan acceptor is nonlabeled XLLG oligosaccharide Arabidopsis thaliana GDP + alpha-L-Gal-xyloglucan
-
 ?
2.4.1.344 additional information no L-galactosyltransferase activities are detected toward XLLG-2-aminopyridine and XXLG-2-aminopyridine Arabidopsis thaliana ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.4.1.69 At2g03220
-
 Arabidopsis thaliana
2.4.1.69 FUT1
-
 Arabidopsis thaliana
2.4.1.344 At2g03220
-
 Arabidopsis thaliana
2.4.1.344 FUT1
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.69 20 25 L-galactosyltransferase activity Arabidopsis thaliana
2.4.1.344 20 25
-
 Arabidopsis thaliana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.69 40
-
 no L-galactosyltransferase activity above Arabidopsis thaliana
2.4.1.344 40
-
 no activity above Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.69 7 7.5 L-galactosyltransferase activity Arabidopsis thaliana
2.4.1.344 7 7.5
-
 Arabidopsis thaliana