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Literature summary extracted from

  • Scaletti, E.; Jemth, A.S.; Helleday, T.; Stenmark, P.
    Structural basis of inhibition of the human serine hydroxymethyltransferase SHMT2 by antifolate drugs (2019), FEBS Lett., 593, 1863-1873 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 expressed in Escherichia coli BL21(DE3)-TIR cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.2.1 sitting drop vapor diffusion method, using 0.1 M Bis-Tris pH 6.5, 28% (w/v) PEGMME 2000 (in complex with lometrexol and methotrexate), 0.2 M LiCl2, 0.1 M MES pH 6.0, 20% (w/v) PEG6000 (in complex with pemetrexed), and 0.2 M NaCl, 0.1 M MES pH 6.0, 20% (w/v) PEG6000 (in complex with raltitrexed) Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.1 lometrexol 49.7% residual activity at 0.1 mM Homo sapiens
2.1.2.1 methotrexate 87.4% residual activity at 0.1 mM Homo sapiens
2.1.2.1 pemetrexed 79.2% residual activity at 0.1 mM Homo sapiens
2.1.2.1 raltitrexed 94% residual activity at 0.1 mM Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.1 mitochondrion
-
 Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Homo sapiens
-
 tetrahydrofolate + L-serine
-
 r
2.1.2.1 tetrahydrofolate + L-serine Homo sapiens
-
 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Homo sapiens P34897
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1 His-Trap column chromatography and Superdex 75 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
 Homo sapiens tetrahydrofolate + L-serine
-
 r
2.1.2.1 tetrahydrofolate + L-serine
-
 Homo sapiens 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.2.1 tetramer
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 serine hydroxymethyltransferase
-
 Homo sapiens
2.1.2.1 SHMT2
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate
-
 Homo sapiens