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Literature summary extracted from

  • Song, H.; Dharmasena, M.N.; Wang, C.; Shaw, G.X.; Cherry, S.; Tropea, J.E.; Jin, D.J.; Ji, X.
    Structure and activity of PPX/GppA homologs from Escherichia coli and Helicobacter pylori (2019), FEBS J., 287, 1865-1885 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.40 expressed in Escherichia coli MG1655 cells Helicobacter pylori
3.6.1.40 expressed in Escherichia coli MG1655 cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.40 sitting drop vapor diffusion method, apoenzyme, using 10% (w/v) PEG 3350, 0.1 M HEPES (pH 7.5), and 0.2 M magnesium chloride Escherichia coli
3.6.1.40 sitting drop vapor diffusion method. Apoenzyme, using 20% (w/v) PEG 3350, 0.2 M ammonium sulfate, and 15 mM malic acid. Enzyme in complex with 5'-guanosine-diphosphate-monothiophosphate or phosphoaminophosphonic acid guanylate ester, using 20% (w/v) PEG 3350 and 0.2 M potassium chloride. Enzyme in complex with guanosine tetraphosphate, using 20% (w/v) PEG 3350, 0.2 M ammonium tartrate, and 15 mM malic acid Helicobacter pylori

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.40 D136A the mutant shows severely reduced activity compared to the wild type enzyme Helicobacter pylori
3.6.1.40 D9A the mutant shows severely reduced activity compared to the wild type enzyme Helicobacter pylori
3.6.1.40 E115A the mutant shows severely reduced activity compared to the wild type enzyme Helicobacter pylori
3.6.1.40 E143A the mutant shows severely reduced activity compared to the wild type enzyme Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.40 0.02443
-
 guanosine 5'-triphosphate 3'-diphosphate at pH 7.4 and 37°C Helicobacter pylori
3.6.1.40 0.04951
-
 guanosine 5'-triphosphate 3'-diphosphate at pH 7.4 and 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.40 Mg2+ required for activity Helicobacter pylori
3.6.1.40 Mg2+ required for activity Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.40 guanosine 5'-triphosphate 3'-diphosphate + H2O Helicobacter pylori
-
 guanosine 3',5'-bis(diphosphate) + phosphate
-
 ?
3.6.1.40 guanosine 5'-triphosphate 3'-diphosphate + H2O Escherichia coli
-
 guanosine 3',5'-bis(diphosphate) + phosphate
-
 ?
3.6.1.40 guanosine 5'-triphosphate 3'-diphosphate + H2O Helicobacter pylori B38
-
 guanosine 3',5'-bis(diphosphate) + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.40 Escherichia coli P25552
-
-
3.6.1.40 Helicobacter pylori C7BYL8
-
-
3.6.1.40 Helicobacter pylori B38 C7BYL8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.40 HisTrap column chromatography and Sephacryl S-200 gel filtration Helicobacter pylori
3.6.1.40 HisTrap column chromatography and Sephacryl S-200 gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.40 guanosine 5'-triphosphate 3'-diphosphate + H2O
-
 Helicobacter pylori guanosine 3',5'-bis(diphosphate) + phosphate
-
 ?
3.6.1.40 guanosine 5'-triphosphate 3'-diphosphate + H2O
-
 Escherichia coli guanosine 3',5'-bis(diphosphate) + phosphate
-
 ?
3.6.1.40 guanosine 5'-triphosphate 3'-diphosphate + H2O
-
 Helicobacter pylori B38 guanosine 3',5'-bis(diphosphate) + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.40 homodimer 2 * 113000, small-angle X-ray scattering Helicobacter pylori
3.6.1.40 homotetramer 2 * 233000, small-angle X-ray scattering Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.6.1.40 GppA
-
 Helicobacter pylori
3.6.1.40 GppA
-
 Escherichia coli
3.6.1.40 pppGpp phosphohydrolase
-
 Helicobacter pylori
3.6.1.40 pppGpp phosphohydrolase
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.40 0.044
-
 guanosine 5'-triphosphate 3'-diphosphate at pH 7.4 and 37°C Helicobacter pylori
3.6.1.40 14.406
-
 guanosine 5'-triphosphate 3'-diphosphate at pH 7.4 and 37°C Escherichia coli