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Literature summary extracted from

  • Kearsey, L.J.; Prandi, N.; Karuppiah, V.; Yan, C.; Leys, D.; Toogood, H.; Takano, E.; Scrutton, N.S.
    Structure of the Cannabis sativa olivetol-producing enzyme reveals cyclization plasticity in type III polyketide synthases (2019), FEBS J., 287, 1511-1524 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.206 expression in Escherichia coli Cannabis sativa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.206 cocrystallization of the enzyme with CoA (2 mM) is performed in 0.2 M potassium nitrate containing 20% w/v PEG 3350 for 72 h at 4 °C using the sitting drop vapour diffusion technique. Crystals are cryo-protected in the mother liquor containing 20% glycerol, followed by flash freezing in liquid nitrogen Cannabis sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.206 3 malonyl-CoA + hexanoyl-CoA Cannabis sativa
-
 3 CoA + 3,5,7-trioxododecanoyl-CoA + 3 CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.206 Cannabis sativa B1Q2B6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.206
-
 Cannabis sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.206 3 malonyl-CoA + hexanoyl-CoA
-
 Cannabis sativa 3 CoA + 3,5,7-trioxododecanoyl-CoA + 3 CO2
-
 ?
2.3.1.206 3 malonyl-CoA + hexanoyl-CoA in the absence of olivetolic acid cyclase (OAC), a nonenzymatic C2 -> C7 decarboxylative aldol condensation of the tetraketide intermediate occurs forming olivetol. The apparent plasticity of the enzyme with only minor alterations in amino acid sequences demonstrates the subtle nature of the mechanistic discrimination. This involves interplay between multiple residues, an inherently flexible active site designed to accommodate a growing scaffold and easily convertible cyclization mechanism(s) with minimal intervention Cannabis sativa 3 CoA + 3,5,7-trioxododecanoyl-CoA + 3 CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.206 tetraketide synthase
-
 Cannabis sativa
2.3.1.206 TKS
-
 Cannabis sativa