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Literature summary extracted from

  • Giardina, G.; Paone, A.; Tramonti, A.; Lucchi, R.; Marani, M.; Magnifico, M.C.; Bouzidi, A.; Pontecorvi, V.; Guiducci, G.; Zamparelli, C.; Rinaldo, S.; Paiardini, A.; Contestabile, R.; Cutruzzola, F.
    The catalytic activity of serine hydroxymethyltransferase is essential for de novo nuclear dTMP synthesis in lung cancer cells (2018), FEBS J., 285, 3238-3253 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.2.1 mutant H135N/R137A/E168N, hanging drop vapor diffusion method, using 0.1 M sodium cacodylate pH 6.5, 1.0 M sodium citrate Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.1.2.1 H135N/R137A dimeric catalytically active mutant Homo sapiens
2.1.2.1 H135N/R137A/E168N tetrameric active mutant with a modified, less stable, tetrameric interface Homo sapiens
2.1.2.1 K257Q/Y82A/Y83F tetrameric inactive mutant Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.1 0.1
-
 L-serine wild type enzyme SHMT1, at pH 7.2 and 20°C Homo sapiens
2.1.2.1 0.314
-
 L-serine mutant H135N/R137A/E168N, at pH 7.2 and 20°C Homo sapiens
2.1.2.1 0.57
-
 L-serine mutant H135N/R137A, at pH 7.2 and 20°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.1 cytosol
-
 Homo sapiens 5829
-
2.1.2.1 nucleus
-
 Homo sapiens 5634
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Homo sapiens
-
 tetrahydrofolate + L-serine
-
 r
2.1.2.1 tetrahydrofolate + L-serine Homo sapiens
-
 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Homo sapiens P34896
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.1 A-549 cell
-
 Homo sapiens
-
2.1.2.1 lung cancer cell
-
 Homo sapiens
-
2.1.2.1 NCI-H1299 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
 Homo sapiens tetrahydrofolate + L-serine
-
 r
2.1.2.1 tetrahydrofolate + L-serine
-
 Homo sapiens 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.2.1 tetramer
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 serine hydroxymethyltransferase
-
 Homo sapiens
2.1.2.1 SHMT1
-
 Homo sapiens
2.1.2.1 SHMT2alpha
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.1.2.1 physiological function the enzyme dynamically changes the fluxes of one-carbon metabolism by reversibly converting L-serine and tetrahydrofolate into 5,10-methylene-tetrahydrofolate and glycine. The cytosolic isoforms can also translocate to the nucleus to sustain de novo thymidylate synthesis and support cell proliferation Homo sapiens