EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.3.2.B14 | 5,5'-dithio-bis-2-nitrobenzoic acid derivative, to 1.55 A resolution. The compound binds to the catalytic cysteine residue, Cys354, in both chains of the asymmetric unit | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.2.B14 | C354A | mutant does not display adduct formation with inhibitors faropenem or 6-aminopenicillanic acid | Mycobacterium tuberculosis |
2.3.2.B14 | H336A | no cysteine acylation by inhibitors can be detected in a 0-500-s time frame | Mycobacterium tuberculosis |
2.3.2.B14 | H352A | mutation does not show a decrease in the rate of the acylation reaction by inhibitors | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.2.B14 | 6-aminopenicillanic acid | high degree of Cys354 modification, occurrence of a possible coupling reaction | Mycobacterium tuberculosis | |
2.3.2.B14 | amoxicillin | no covalent adduct observed | Mycobacterium tuberculosis | |
2.3.2.B14 | biapenem | high degree of Cys354 modification | Mycobacterium tuberculosis | |
2.3.2.B14 | doripenem | high degree of Cys354 modification | Mycobacterium tuberculosis | |
2.3.2.B14 | ertapenem | high degree of Cys354 modification | Mycobacterium tuberculosis | |
2.3.2.B14 | faropenem | high degree of Cys354 modification, fast degradation following the initial acylation event | Mycobacterium tuberculosis | |
2.3.2.B14 | faropenem daloxate | high degree of Cys354 modification, fast degradation following the initial acylation event | Mycobacterium tuberculosis | |
2.3.2.B14 | meropenem | high degree of Cys354 modification | Mycobacterium tuberculosis | |
2.3.2.B14 | additional information | the intact catalytic site is required for the acylation by beta-lactams | Mycobacterium tuberculosis | |
2.3.2.B14 | penicillin-G | no covalent adduct observed | Mycobacterium tuberculosis | |
2.3.2.B14 | piperacillin | high degree of Cys354 modification | Mycobacterium tuberculosis | |
2.3.2.B14 | pivmecillinam | no covalent adduct observed | Mycobacterium tuberculosis | |
2.3.2.B14 | tebipenem | high degree of Cys354 modification | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.2.B14 | Mycobacterium tuberculosis | I6Y9J2 | - |
- |
2.3.2.B14 | Mycobacterium tuberculosis H37Rv | I6Y9J2 | - |
- |