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Literature summary extracted from

  • Garcia, M.D.; Wang, J.G.; Lonhienne, T.; Guddat, L.W.
    Crystal structure of plant acetohydroxyacid synthase, the target for several commercial herbicides (2017), FEBS J., 284, 2037-2051 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.6 expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae
2.2.1.6 expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.2.1.6 free enzyme and in complex with inhibitors, hanging drop vapor diffusion method, using 1 M sodium potassium tartrate, 0.1 M CHES and 0.1-0.2 M lithium sulfate Saccharomyces cerevisiae
2.2.1.6 free enzyme and in complex with inhibitors, hanging drop vapor diffusion method, using 1 M sodium potassium tartrate, 0.1 M CHES and 0.1-0.2 M lithium sulfate Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.6 bispyribac-sodium
-
 Arabidopsis thaliana
2.2.1.6 bispyribac-sodium
-
 Saccharomyces cerevisiae
2.2.1.6 chlorimuron ethyl
-
 Arabidopsis thaliana
2.2.1.6 chlorimuron ethyl
-
 Saccharomyces cerevisiae
2.2.1.6 chlorsulfuron
-
 Arabidopsis thaliana
2.2.1.6 chlorsulfuron
-
 Saccharomyces cerevisiae
2.2.1.6 florasulam
-
 Arabidopsis thaliana
2.2.1.6 florasulam
-
 Saccharomyces cerevisiae
2.2.1.6 imazapyr
-
 Arabidopsis thaliana
2.2.1.6 imazapyr
-
 Saccharomyces cerevisiae
2.2.1.6 imazaquin
-
 Arabidopsis thaliana
2.2.1.6 imazaquin
-
 Saccharomyces cerevisiae
2.2.1.6 imazethapyr
-
 Arabidopsis thaliana
2.2.1.6 imazethapyr
-
 Saccharomyces cerevisiae
2.2.1.6 thiencarbazone methyl
-
 Arabidopsis thaliana
2.2.1.6 thiencarbazone methyl
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.6 Mg2+ required Saccharomyces cerevisiae
2.2.1.6 Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.6 2 pyruvate Saccharomyces cerevisiae
-
 2-acetolactate + CO2
-
 ?
2.2.1.6 2 pyruvate Arabidopsis thaliana
-
 2-acetolactate + CO2
-
 ?
2.2.1.6 2-oxobutyrate + pyruvate Saccharomyces cerevisiae
-
 (S)-2-aceto-2-hydroxybutyrate + CO2
-
 ?
2.2.1.6 2-oxobutyrate + pyruvate Arabidopsis thaliana
-
 (S)-2-aceto-2-hydroxybutyrate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Arabidopsis thaliana P17597
-
-
2.2.1.6 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.6 Ni-NTA resin column chromatography and S-200 HR gel filtration Saccharomyces cerevisiae
2.2.1.6 Ni-NTA resin column chromatography and S-200 HR gel filtration Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 2 pyruvate
-
 Saccharomyces cerevisiae 2-acetolactate + CO2
-
 ?
2.2.1.6 2 pyruvate
-
 Arabidopsis thaliana 2-acetolactate + CO2
-
 ?
2.2.1.6 2-oxobutyrate + pyruvate
-
 Saccharomyces cerevisiae (S)-2-aceto-2-hydroxybutyrate + CO2
-
 ?
2.2.1.6 2-oxobutyrate + pyruvate
-
 Arabidopsis thaliana (S)-2-aceto-2-hydroxybutyrate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.6 homodimer
-
 Saccharomyces cerevisiae
2.2.1.6 homotetramer
-
 Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.2.1.6 acetohydroxyacid synthase
-
 Saccharomyces cerevisiae
2.2.1.6 acetohydroxyacid synthase
-
 Arabidopsis thaliana
2.2.1.6 AHAS
-
 Saccharomyces cerevisiae
2.2.1.6 AHAS
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.6 FAD
-
 Saccharomyces cerevisiae
2.2.1.6 FAD
-
 Arabidopsis thaliana
2.2.1.6 thiamine diphosphate
-
 Saccharomyces cerevisiae
2.2.1.6 thiamine diphosphate
-
 Arabidopsis thaliana