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Literature summary extracted from

  • Su D., Asard H.
    Three mammalian cytochromes b561 are ascorbate-dependent ferrireductases (2006), FEBS J., 273, 3722-3374 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.1.3 expressed in a Saccharomyces cerevisiae DELTAfre1DELTAfre2 mutant, which lacks almost all of its plasma membrane ferrireductase activity Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
7.2.1.3 D38A the mutation of lysosomal cytochrome b561 increases the enzyme activity compared to the wild type Mus musculus
7.2.1.3 E177A the mutation of lysosomal cytochrome b561 reduces the enzyme activity compared to the wild type Mus musculus
7.2.1.3 E196A the mutation of lysosomal cytochrome b561 strongly reduces the enzyme activity compared to the wild type Mus musculus
7.2.1.3 F44A the mutation of lysosomal cytochrome b561 reduces the enzyme activity by about 45% compared to the wild type Mus musculus
7.2.1.3 H105A the mutation of lysosomal cytochrome b561 increases the enzyme activity compared to the wild type Mus musculus
7.2.1.3 H112A the mutation of lysosomal cytochrome b561 reduces the enzyme activity compared to the wild type Mus musculus
7.2.1.3 H117A the mutation of lysosomal cytochrome b561 completely abrogates the FeCN reductase activity of the enzyme Mus musculus
7.2.1.3 H156A the mutation of lysosomal cytochrome b561 completely abrogates the FeCN reductase activity of the enzyme Mus musculus
7.2.1.3 H47A the mutation of lysosomal cytochrome b561 completely abrogates the FeCN reductase activity of the enzyme Mus musculus
7.2.1.3 H83A the mutation of lysosomal cytochrome b561 completely abrogates the FeCN reductase activity of the enzyme Mus musculus
7.2.1.3 M51A the mutation of lysosomal cytochrome b561 increases the enzyme activity compared to the wild type Mus musculus
7.2.1.3 N106A the mutation of lysosomal cytochrome b561 increases the enzyme activity compared to the wild type Mus musculus
7.2.1.3 P48A the mutation of lysosomal cytochrome b561 increases the enzyme activity compared to the wild type Mus musculus
7.2.1.3 Q131A the mutation of lysosomal cytochrome b561 reduces the enzyme activity to 45% of that of the wild type Mus musculus
7.2.1.3 R149A the mutation of lysosomal cytochrome b561 results in a 75% loss in activity compared to the wild type enzyme Mus musculus
7.2.1.3 R67A the mutation of lysosomal cytochrome b561 results in an almost complete loss of the FeCN reductase activity of the enzyme Mus musculus
7.2.1.3 S115A the ferrireductase activity of lysosomal cytochrome b561 in mutant S115A is reduced to 50% compared to the wild type Mus musculus
7.2.1.3 S118A the mutation of lysosomal cytochrome b561 increases the enzyme activity compared to the wild type Mus musculus
7.2.1.3 W119A the ferrireductase activity of lysosomal cytochrome b561 in mutant W119A is reduced to 17% compared to the wild type Mus musculus
7.2.1.3 Y190A the mutation of lysosomal cytochrome b561 increases the enzyme activity compared to the wild type Mus musculus
7.2.1.3 Y66A the mutation of lysosomal cytochrome b561 results in an almost complete loss of the FeCN reductase activity of the enzyme Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.1.3 plasma membrane a transmembrane protein Mus musculus 5886
-

Organism

EC Number Organism UniProt Comment Textmining
7.2.1.3 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.2.1.3 duodenum duodenal cytochrome b561 Mus musculus
-
7.2.1.3 kidney chromaffin granule cytochrome b561 and lysosomal cytochrome b561 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.1.3 ascorbate[side 1] + Fe3+-EDTA[side 2] compared with FeCN, Fe3+-EDTA is a relatively poor substrate for the enzyme (20-35fold lower ferrireductase activities) Mus musculus monodehydroascorbate[side 1] + Fe2+-EDTA[side 2]
-
 ?
7.2.1.3 ascorbate[side 1] + ferricyanide[side 2]
-
 Mus musculus monodehydroascorbate[side 1] + ferrocyanide[side2]
-
-

Subunits

EC Number Subunits Comment Organism
7.2.1.3 ? x * 27000, lysosomal cytochrome b561, SDS-PAGE Mus musculus
7.2.1.3 ? x * 27800, chromaffin granule cytochrome b561, SDS-PAGE Mus musculus
7.2.1.3 ? x * 31500, duodenal cytochrome b561, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
7.2.1.3 ascorbate-dependent ferrireductase
-
 Mus musculus
7.2.1.3 CGCytb
-
 Mus musculus
7.2.1.3 chromaffin granule cytochrome b561
-
 Mus musculus
7.2.1.3 Cyt b561
-
 Mus musculus
7.2.1.3 cytochrome b561
-
 Mus musculus
7.2.1.3 DCytb
-
 Mus musculus
7.2.1.3 duodenal cytochrome b561
-
 Mus musculus
7.2.1.3 LCytb
-
 Mus musculus
7.2.1.3 lysosomal cytochrome b561
-
 Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
7.2.1.3 ascorbate the enzyme is dependent on the presence of intracellular ascorbate Mus musculus
7.2.1.3 heme a di-heme enzyme Mus musculus