Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lee, M.N.; Kweon, H.Y.; Oh, G.T.
    N-alpha-acetyltransferase 10 (NAA10) in development the role of NAA10 (2018), Exp. Mol. Med., 50, 1-11 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.255 F128I site-directed mutagenesis, the mutation leads an altered structure and reduced stability, and a dramatic recuction of Nt catalytic activity compared to wild-type Homo sapiens
2.3.1.255 F128L site-directed mutagenesis, the mutation leads an altered structure and reduced stability, and a dramatic recuction of Nt catalytic activity compared to wild-type Homo sapiens
2.3.1.255 additional information enzyme knockdown by siRNA. Generation of mutant daf-31(m655) by removal of 151 bp of promoter upstream of the ATG start codon and 242 bp of daf-31 coding region dowstream of the ATG start codon. Generation of and overexpression mutant daf-31 OE for which the full-length dar-31 genomic DNA is cloned into pGEM-T vector. Generation of mutant vncBDk by with impaired N-terminal activity Caenorhabditis elegans
2.3.1.255 additional information generation of an Ard1 null mutant by removal of the ARD1 coding region Trypanosoma brucei
2.3.1.255 additional information generation of enzyme mutant by frame shift mutation causing a acetyltransferase-truncated enzymatic region, and of another mutant vnc by intron-insertion mutation Drosophila melanogaster
2.3.1.255 additional information generation of mutant naa10-1 and of a naa15 mutant by T-DNA insertion-disrupting gene expression Arabidopsis thaliana
2.3.1.255 additional information generation of mutant naa10MO by morpholino-based knockdown Danio rerio
2.3.1.255 additional information mutation ard1::HIS3 is caused by inserting a Barn HI fragment containinng the HIS3 gene into the Barn HI site of plasmid YCpE18 that lies within the functional sequence of ARD1. Genes nat1/ard1 double mutant nat1-5::LEU;ard1 is generated by mating of nat1 and ard1 single mutants, the single mutants of nat1 (Naa15) and ard1 (Naa10) display identical phenotypes, no additional phenotypes are found in the double mutant. Recombinant expression of the S37P mutant of human Naa10 in a NatA-defective yeast strain, the hNaa10 expressing mutant strain shows a lack of proper complex formation with hNaa15 and reduced in vitro catalytic activity, a decrease of Nt-acetylome and an increase in the Hsp70 family proteins Saccharomyces cerevisiae
2.3.1.255 R116W site-directed mutagenesis, the mutation leads to a reduction in catalytic activity for the peptide substrates EEEI and SESS by 15% compared to wild-type Homo sapiens
2.3.1.255 R83C site-directed mutagenesis, the mutation interferes with acetyl-CoA binding and leads to a 60% reduction in Nt-catalytic activity compared to wild-type Homo sapiens
2.3.1.255 S37P site-directed mutagenesis, the mutant Naa10 protein shows reduced catalytic activity for EEEI, DDDI, and SESS peptide substrates, and inability to combine with Naa15. The mutant hNaa10 S37P recombinantly expressed in a NatA-defective Saccharomyces cerevisiae strain lacks a proper complex formation with hNaa15 and is reduced in in vitro catalytic activity Homo sapiens
2.3.1.255 S39P site-directed mutagenesis, the mutation does not cause a phenotype Saccharomyces cerevisiae
2.3.1.255 V107F site-directed mutagenesis, the mutation leads to a reduction in catalytic activity for the peptide substrates EEEI and SESS by 95% compared to wild-type Homo sapiens
2.3.1.255 Y43S site-directed mutagenesis, the mutant is catalytically impaired in vitro, with approximately an 85% reduction in Nt-catalytic activity for peptide substrates EEEI, DDDI, and SESS Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.255 Arabidopsis thaliana Q9FKI4
-
-
2.3.1.255 Caenorhabditis elegans O61219
-
-
2.3.1.255 Caenorhabditis elegans DAF-31 O61219
-
-
2.3.1.255 Danio rerio Q7T3B8
-
-
2.3.1.255 Drosophila melanogaster Q9VT75
-
-
2.3.1.255 Homo sapiens P41227
-
-
2.3.1.255 Mus musculus Q3UX61
-
-
2.3.1.255 Mus musculus Q9QY36
-
-
2.3.1.255 Mus musculus C57Bl6/J Q3UX61
-
-
2.3.1.255 Mus musculus C57Bl6/J Q9QY36
-
-
2.3.1.255 Saccharomyces cerevisiae P07347 AND P12945 subunits Naa10 and Naa15 of enzmye complex NatA
-
2.3.1.255 Saccharomyces cerevisiae ATCC 204508 P07347 AND P12945 subunits Naa10 and Naa15 of enzmye complex NatA
-
2.3.1.255 Trypanosoma brucei Q9NFL8
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.255 bone
-
 Mus musculus
-
2.3.1.255 brain during brain development in mouse, Naa10 and Naa15 (the auxiliary subunit of NatA) are highly expressed in regions rich in proliferating and migrating cells, such as the ventricular zone, neocortex, olfactory bulb, and hippocampus. The expression of both genes is downregulated as neurons differentiate and mitotic and migratory activities subside. Then, once again, their expression increases during postnatal development in the hippocampus and during the neuronal dendritic development of Purkinje cells (PCs) in the cerebellum. This finding indicates that the regulation of expression of both genes is related to neuronal development, especially in the hippocampus and in the PCs of the cerebellum Mus musculus
-
2.3.1.255 cerebellar Purkinje cell
-
 Mus musculus
-
2.3.1.255 colon
-
 Homo sapiens
-
2.3.1.255 colon
-
 Mus musculus
-
2.3.1.255 hippocampus
-
 Mus musculus
-
2.3.1.255 kidney
-
 Homo sapiens
-
2.3.1.255 kidney
-
 Mus musculus
-
2.3.1.255 liver
-
 Homo sapiens
-
2.3.1.255 liver
-
 Mus musculus
-
2.3.1.255 lung
-
 Homo sapiens
-
2.3.1.255 lung
-
 Mus musculus
-
2.3.1.255 additional information NAA10 and NAA11 display opposite expression patterns during spermatogenesis. No expression of Naa11 in premeiotic spermatogonia Mus musculus
-
2.3.1.255 additional information tissue-specific expression of Naa10 during different developmental stages in C57BL6/J mice Mus musculus
-
2.3.1.255 additional information tissue-specific expression of Naa10 during different developmental stages in humans Homo sapiens
-
2.3.1.255 NB-4 cell NAA10 expression decreases with the induction of differentiation in NB4 cells, but the level of NAA11 remains unchanged Mus musculus
-
2.3.1.255 neuron
-
 Mus musculus
-
2.3.1.255 occipital lobe
-
 Homo sapiens
-
2.3.1.255 occipital lobe
-
 Mus musculus
-
2.3.1.255 olfactory bulb
-
 Mus musculus
-
2.3.1.255 osteoblast
-
 Mus musculus
-
2.3.1.255 parietal lobe
-
 Homo sapiens
-
2.3.1.255 parietal lobe
-
 Mus musculus
-
2.3.1.255 skin
-
 Homo sapiens
-
2.3.1.255 skin
-
 Mus musculus
-
2.3.1.255 spermatogonium Naa10 is expressed in premeiotic spermatogonia Mus musculus
-
2.3.1.255 spinal cord
-
 Homo sapiens
-
2.3.1.255 spinal cord
-
 Mus musculus
-
2.3.1.255 testis NAA11, a homologue of NAA10, is predominantly expressed in mouse testis Mus musculus
-
2.3.1.255 testis NAA11, a homologue of NAA10, is predominantly expressed in mouse testis. NAA10 and NAA11 display opposite expression patterns during spermatogenesis Mus musculus
-
2.3.1.255 tongue
-
 Homo sapiens
-
2.3.1.255 tongue
-
 Mus musculus
-
2.3.1.255 uterus
-
 Homo sapiens
-
2.3.1.255 uterus
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.255 acetyl-CoA + N-terminal L-aspartyl-[DDIAALRWGRPVGRRRRPVRVYP]
-
 Homo sapiens CoA + H+ + N-terminal Nalpha-acetyl-L-aspartyl-[DDDIAALRWGRPVGRRRRPVRVYP]
-
 ?
2.3.1.255 acetyl-CoA + N-terminal L-glutamyl-[EEIAALRWGRPVGRRRRPVRVYP]
-
 Homo sapiens CoA + H+ + N-terminal Nalpha-acetyl-L-glutamyl-[EEEIAALRWGRPVGRRRRPVRVYP]
-
 ?
2.3.1.255 acetyl-CoA + N-terminal L-methionyl-[LGPEGGRWGRPVGRRRRPVRVYP]
-
 Homo sapiens CoA + H+ + N-terminal Nalpha-acetyl-L-methionyl-[MLGPEGGRWGRPVGRRRRPVRVYP]
-
 ?
2.3.1.255 acetyl-CoA + N-terminal L-seryl-[ESSSKSRWGRPVGRRRRPVRVYP]
-
 Homo sapiens CoA + H+ + N-terminal Nalpha-acetyl-L-seryl-[SESSSKSRWGRPVGRRRRPVRVYP]
-
 ?
2.3.1.255 additional information acetylation of alpha-tubulin Mus musculus ?
-
-
2.3.1.255 additional information enzyme zNaa10 has a predicted N-terminal activity with identical substrate specificity to human Naa10 in vitro Danio rerio ?
-
-
2.3.1.255 additional information acetylation of alpha-tubulin Mus musculus C57Bl6/J ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.3.1.255 ARD1
-
 Danio rerio
2.3.1.255 ARD1
-
 Trypanosoma brucei
2.3.1.255 ARD1
-
 Drosophila melanogaster
2.3.1.255 ARD1
-
 Saccharomyces cerevisiae
2.3.1.255 Ard1b
-
 Mus musculus
2.3.1.255 ARD2
-
 Mus musculus
2.3.1.255 AtNAA10
-
 Arabidopsis thaliana
2.3.1.255 daf-31
-
 Caenorhabditis elegans
2.3.1.255 N-alpha-acetyltransferase
-
 Caenorhabditis elegans
2.3.1.255 N-alpha-acetyltransferase 10
-
 Homo sapiens
2.3.1.255 N-alpha-acetyltransferase 10
-
 Danio rerio
2.3.1.255 N-alpha-acetyltransferase 10
-
 Mus musculus
2.3.1.255 N-alpha-acetyltransferase 10
-
 Arabidopsis thaliana
2.3.1.255 N-alpha-acetyltransferase 11
-
 Mus musculus
2.3.1.255 NAA10
-
 Homo sapiens
2.3.1.255 NAA10
-
 Danio rerio
2.3.1.255 NAA10
-
 Mus musculus
2.3.1.255 NAA10
-
 Arabidopsis thaliana
2.3.1.255 NAA11
-
 Mus musculus
2.3.1.255 NAT1
-
 Saccharomyces cerevisiae
2.3.1.255 TbARD1
-
 Trypanosoma brucei
2.3.1.255 vnc
-
 Caenorhabditis elegans
2.3.1.255 yNaa10
-
 Saccharomyces cerevisiae
2.3.1.255 yNaa15
-
 Saccharomyces cerevisiae
2.3.1.255 zNaa10
-
 Danio rerio

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.255 acetyl-CoA
-
 Homo sapiens

Expression

EC Number Organism Comment Expression
2.3.1.255 Mus musculus in mouse, the expression of Naa10 is downregulated during meiosis down
2.3.1.255 Mus musculus in mouse, the expression of Naa11 is upregulated during meiosis up

General Information

EC Number General Information Comment Organism
2.3.1.255 malfunction enzyme knockdown causes a phenotype with lethality, growth retardation, bent axis and tails, abnormal eyes, and less pigmentation Danio rerio
2.3.1.255 malfunction enzyme mutants show phenotypes with pleiotropic oogenesis, aberrant mitosis, egg chamber encapsulation defects, and nurse cell chromatin dispersion defects Drosophila melanogaster
2.3.1.255 malfunction inactive Naa10 mutant S37Pw shows a phenotype with perinatal lethal disorder, hypotonia, global developmental delay, cryptorchidism, cardiac arrhythmias, skin laxity, dysmorphic features, hernias, and large fontanels. Naa10 mutant Y43S shows a phenotype with intellectual disability, facial dysmorphism, scoliosis, and long QT. Mutant R83C shows a phenootype with hypotonia, global developmental delay, dysmorphic features, autism spectrum disorder, epileptic encephalopathy, extrapyramidal signs, hypertension with left ventricular hypertrophy, thin corpus callosum, and progressive white matter loss. Mutations V107F and R116W cause phenotypes with severe global developmental delay with postnatal growth, skeletal anomalies, truncal hypotonia with hypertonia of the extremities, minor facial features, and behavioral anomalies. Mutation of residue F128 causes moderate to severe intellectually disability, feeding difficulties, eye anomalies, hypotonia, and developmental delay Homo sapiens
2.3.1.255 malfunction mutation ard1::HIS3 leads to a defect in transcription of a-specific genes, but permits expression of the information resident at HML. The mutant shows a phenotypes with reduced viability and sensitivity to heat shock and salt, it fails to enter stationay phase, it shows a lack of glycogen accumulation, a sporulation defect, poor mating, and fails to undergo meiosis. The mutant nat1-5::LEU;ard1 is inable to sporulate, has slow growth, reduced mating, inhibited sporulation, and impaired resistance to heat shock. It fails G1 arrest, shows a partial depression of HML, and fails to accumulate storage. yNaa10 deficiency leads to a growth defect, sensitivity to caffeine and cycloheximide, impaired resistance to heat shock, and decreased mating efficiency Saccharomyces cerevisiae
2.3.1.255 malfunction overexpression of gene daf-31 causes an increased lifespan in daf-2 mutant enhancing reproduction, while daf-31 knockdown by siRNA causes a decreased lifespan Caenorhabditis elegans
2.3.1.255 malfunction overexpression of Naa10 in mice results in the delayed closure of calvarial fontanels and reduced bone density, osteoblast surfaces and mRNA levels of the osteoblastogenic genes in calvaria. In contrast, Naa10 deficient mice display calvarial and femoral bone development to a greater extent on postnatal day 3 Mus musculus
2.3.1.255 malfunction the ARD1 null mutation leads to impaired growth in bloodstream-form cells and reduced differentiation to insect-stage cells Trypanosoma brucei
2.3.1.255 malfunction the naa10 knockout mutant naa10-1 shows growth retardation in vegetative stage, abortion of embryogenesis, and drought-adapted root morphology, the mutation is lethal. A knockout of naa15 causes the same phenotype Arabidopsis thaliana
2.3.1.255 physiological function enzyme Daf-31 regulates the transcriptional activity of DAF-16, the FOXO transcription factor. Mutant daf-31(m655) leads to developmental larval arrest, fat accumulation, formation of dauer-like larvae under starvation conditions, and decreased lifespan, and the mutant lacks SDS-resistance and cannot resume development and reproduction after food re-providing Caenorhabditis elegans
2.3.1.255 physiological function importance of NAA10 catalytic activity in human development. The potential role of NAA10 varies depending on transcriptional levels in different tissues and embryonic stages during development Homo sapiens
2.3.1.255 physiological function importance of NAA10 catalytic activity in mouse development. The potential role of NAA10 varies depending on transcriptional levels in different tissues and embryonic stages during development. Naa10 homologue Naa11 has a role in the cellular differentiation process while Naa10 has a role in the cellular proliferation process. The differential expression pattern of Naa10 and Naa11 suggests that Naa11 is complementary to Naa10 at least in the mice and that its biological role can be important in spermiogenesis or cellular processes Mus musculus
2.3.1.255 physiological function importance of NAA10 catalytic activity in mouse development. The potential role of NAA10 varies depending on transcriptional levels in different tissues and embryonic stages during development. Naa10 homologue Naa11 has a role in the cellular differentiation process while Naa10 has a role in the cellular proliferation process. The differential expression pattern of Naa10 and Naa11 suggests that Naa11 is complementary to Naa10 at least in the mice and that its biological role can be important in spermiogenesis or cellular processes. Naa10 is known to regulate cellular processes, and its effects are not only catalyzed through its major activity as a NAT but also through the N-epsilon-acetylation of several proteins. The N-epsilon-acetyl-activity of Naa10 requires auto-acetylation. This requirement is similar to that of other acetyltransferases, which acetylate themselves for their catalytic and functional activities. Naa10 plays an important role in osteoblast differentiation and the early phases of bone formation. Naa10 counteracts HDAC6 by acetylating alpha-tubulin, thereby promoting MT stability for dendritic development Mus musculus
2.3.1.255 physiological function Naa10 is crucial for cell growth and sporulation Saccharomyces cerevisiae