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Literature summary extracted from

  • Payongsri, P.; Steadman, D.; Hailes, H.C.; Dalby, P.A.
    Second generation engineering of transketolase for polar aromatic aldehyde substrates (2015), Enzyme Microb. Technol., 71, 45-52 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.2.1.1 D469T the mutant shows higher activity with 3-formylbenzoic acid and 4-formylbenzoic acid compared to the wild type enzyme Escherichia coli
2.2.1.1 D469T/R520Q the mutant shows 80% activity with 3-formylbenzoic acid, 50% activity with 4-formylbenzoic acid and no activity with 3-hydroxybenzaldehyde compared to mutant enzyme D469T Escherichia coli
2.2.1.1 S385E the mutation completely removes the substrate inhibition for 3-formylbenzoic acid Escherichia coli
2.2.1.1 S385E/D469T/R520Q the mutant shows 350% activity with 3-formylbenzoic acid, 20% activity with 4-formylbenzoic acid and 600% activity activity with 3-hydroxybenzaldehyde compared to mutant enzyme D469T Escherichia coli
2.2.1.1 S385T/D469T/R520Q the mutant shows 50% activity with 3-formylbenzoic acid, 210% activity with 4-formylbenzoic acid and 1270% activity activity with 3-hydroxybenzaldehyde compared to mutant enzyme D469T Escherichia coli
2.2.1.1 S385Y/D469T/R520Q the mutant shows 50% activity with 3-formylbenzoic acid, 340% activity with 4-formylbenzoic acid and 1240% activity activity with 3-hydroxybenzaldehyde compared to mutant enzyme D469T Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.1 3-formylbenzoic acid substrate inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.1 1.3
-
 3-formylbenzoic acid mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 1.7
-
 3-formylbenzoic acid mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 2 3 4-formylbenzoic acid mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 13
-
 3-formylbenzoic acid mutant enzyme D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 13
-
 4-formylbenzoic acid mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 18
-
 3-formylbenzoic acid mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 25
-
 4-formylbenzoic acid mutant enzyme D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 56
-
 3-formylbenzoic acid mutant enzyme D469T, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 72
-
 4-formylbenzoic acid mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 180
-
 3-Hydroxybenzaldehyde mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 245
-
 3-Hydroxybenzaldehyde mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 251
-
 4-formylbenzoic acid mutant enzyme D469T, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 390
-
 3-Hydroxybenzaldehyde mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.1 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.1 3-formylbenzoic acid + hydroxypyruvate
-
 Escherichia coli 3-(1,3-dihydroxy-2-oxopropyl)benzoic acid + CO2
-
 ?
2.2.1.1 3-hydroxybenzaldehyde + hydroxypyruvate
-
 Escherichia coli 1,3-dihydroxy-1-(3-hydroxyphenyl)propan-2-one + CO2
-
 ?
2.2.1.1 4-formylbenzoic acid + hydroxypyruvate
-
 Escherichia coli 4-(1,3-dihydroxy-2-oxopropyl)benzoic acid + CO2
-
 ?
2.2.1.1 propanal + glycolaldehyde
-
 Escherichia coli (3S)-1,3-dihydroxypentan-2-one
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.2.1.1 TktA
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.2.1.1 0.2
-
 4-formylbenzoic acid mutant enzyme D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 0.3
-
 4-formylbenzoic acid mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 0.6
-
 3-Hydroxybenzaldehyde mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 1
-
 3-Hydroxybenzaldehyde mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 1.7
-
 4-formylbenzoic acid mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 2.1
-
 4-formylbenzoic acid mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 2.1
-
 3-Hydroxybenzaldehyde mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 5
-
 4-formylbenzoic acid mutant enzyme D469T, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 6
-
 3-formylbenzoic acid mutant enzyme D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 7
-
 3-formylbenzoic acid mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 7.3
-
 3-formylbenzoic acid mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 13.2
-
 3-formylbenzoic acid mutant enzyme D469T, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 34
-
 3-formylbenzoic acid mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.1 thiamine diphosphate
-
 Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.2.1.1 0.0024
-
 3-Hydroxybenzaldehyde mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 0.0046
-
 4-formylbenzoic acid mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 0.0054
-
 3-Hydroxybenzaldehyde mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 0.0055
-
 3-Hydroxybenzaldehyde mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 0.0081
-
 4-formylbenzoic acid mutant enzyme D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 0.02
-
 4-formylbenzoic acid mutant enzyme D469T, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 0.09
-
 4-formylbenzoic acid mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 0.11
-
 4-formylbenzoic acid mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 0.24
-
 3-formylbenzoic acid mutant enzyme D469T, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 0.47
-
 3-formylbenzoic acid mutant enzyme D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 1.9
-
 3-formylbenzoic acid mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 4.25
-
 3-formylbenzoic acid mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.2.1.1 5.4
-
 3-formylbenzoic acid mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli