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Literature summary extracted from

  • Choe, H.; Cha, M.; Stewart, J.
    Semi-rational approach to expand the acyl-CoA chain length tolerance of Sphingomonas paucimobilis serine palmitoyltransferase (2020), Enzyme Microb. Technol., 137, 109515 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.50 expressed in Escherichia coli BL21-Gold (DE3) cells Sphingomonas paucimobilis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.50 R378K the mutant shows lower specific activities for myristoyl-CoA and palmitoyl-CoA but greater efficiencies for caproyl- and lauroyl-CoA compared to the wild type enzyme Sphingomonas paucimobilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.50 0.024
-
 palmitoyl-CoA mutant enzyme R378K, at pH 7.5 and 25°C Sphingomonas paucimobilis
2.3.1.50 0.041
-
 palmitoyl-CoA wild type enzyme, at pH 7.5 and 25°C Sphingomonas paucimobilis
2.3.1.50 0.08
-
 myristoyl-CoA wild type enzyme, at pH 7.5 and 25°C Sphingomonas paucimobilis
2.3.1.50 0.097
-
 myristoyl-CoA mutant enzyme R378K, at pH 7.5 and 25°C Sphingomonas paucimobilis
2.3.1.50 0.25
-
 lauroyl-CoA wild type enzyme, at pH 7.5 and 25°C Sphingomonas paucimobilis
2.3.1.50 0.56
-
 lauroyl-CoA mutant enzyme R378K, at pH 7.5 and 25°C Sphingomonas paucimobilis
2.3.1.50 0.68
-
 caproyl-CoA wild type enzyme, at pH 7.5 and 25°C Sphingomonas paucimobilis
2.3.1.50 0.9
-
 caproyl-CoA mutant enzyme R378K, at pH 7.5 and 25°C Sphingomonas paucimobilis
2.3.1.50 5.6
-
 L-serine mutant enzyme R378K, with palmitoyl-CoA as cosubstrate, at pH 7.5 and 25°C Sphingomonas paucimobilis
2.3.1.50 7.2
-
 L-serine wild type enzyme, with palmitoyl-CoA as cosubstrate, at pH 7.5 and 25°C Sphingomonas paucimobilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.50 palmitoyl-CoA + L-serine Sphingomonas paucimobilis
-
 CoA + 3-dehydro-D-sphinganine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.50 Sphingomonas paucimobilis Q93UV0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.50 Ni-NTA column chromatography Sphingomonas paucimobilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.50 caproyl-CoA + L-serine very low activity Sphingomonas paucimobilis ?
-
 ?
2.3.1.50 lauroyl-CoA + L-serine 18% activity compared to palmitoyl-CoA Sphingomonas paucimobilis CoA + 2-amino-1-hydroxytetradecan-3-one + CO2
-
 ?
2.3.1.50 additional information no activity with octanoyl-CoA Sphingomonas paucimobilis ?
-
-
2.3.1.50 myristoyl-CoA + L-serine second best substrate Sphingomonas paucimobilis CoA + 2-amino-1-hydroxyhexadecan-3-one + CO2
-
 ?
2.3.1.50 palmitoyl-CoA + L-serine
-
 Sphingomonas paucimobilis CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
2.3.1.50 palmitoyl-CoA + L-serine 100% activity Sphingomonas paucimobilis CoA + 3-dehydro-D-sphinganine + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.50 serine palmitoyltransferase
-
 Sphingomonas paucimobilis
2.3.1.50 SPTase
-
 Sphingomonas paucimobilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.50 0.034
-
 caproyl-CoA wild type enzyme, at pH 7.5 and 25°C Sphingomonas paucimobilis
2.3.1.50 0.11
-
 lauroyl-CoA wild type enzyme, at pH 7.5 and 25°C Sphingomonas paucimobilis
2.3.1.50 0.12
-
 palmitoyl-CoA mutant enzyme R378K, at pH 7.5 and 25°C Sphingomonas paucimobilis
2.3.1.50 0.13
-
 caproyl-CoA mutant enzyme R378K, at pH 7.5 and 25°C Sphingomonas paucimobilis
2.3.1.50 0.19
-
 myristoyl-CoA mutant enzyme R378K, at pH 7.5 and 25°C Sphingomonas paucimobilis
2.3.1.50 0.35
-
 lauroyl-CoA mutant enzyme R378K, at pH 7.5 and 25°C Sphingomonas paucimobilis
2.3.1.50 0.76
-
 myristoyl-CoA wild type enzyme, at pH 7.5 and 25°C Sphingomonas paucimobilis
2.3.1.50 0.85
-
 palmitoyl-CoA wild type enzyme, at pH 7.5 and 25°C Sphingomonas paucimobilis

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.50 pyridoxal 5'-phosphate
-
 Sphingomonas paucimobilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.50 0.05
-
 caproyl-CoA wild type enzyme, at pH 7.5 and 25°C Sphingomonas paucimobilis
2.3.1.50 0.14
-
 caproyl-CoA mutant enzyme R378K, at pH 7.5 and 25°C Sphingomonas paucimobilis
2.3.1.50 0.44
-
 lauroyl-CoA wild type enzyme, at pH 7.5 and 25°C Sphingomonas paucimobilis
2.3.1.50 0.625
-
 lauroyl-CoA mutant enzyme R378K, at pH 7.5 and 25°C Sphingomonas paucimobilis
2.3.1.50 1.2
-
 L-serine mutant enzyme R378K, with myristoyl-CoA as cosubstrate, at pH 7.5 and 25°C Sphingomonas paucimobilis
2.3.1.50 1.4
-
 L-serine wild type enzyme, with myristoyl-CoA as cosubstrate, at pH 7.5 and 25°C Sphingomonas paucimobilis
2.3.1.50 2
-
 myristoyl-CoA mutant enzyme R378K, at pH 7.5 and 25°C Sphingomonas paucimobilis
2.3.1.50 5
-
 palmitoyl-CoA mutant enzyme R378K, at pH 7.5 and 25°C Sphingomonas paucimobilis
2.3.1.50 9.5
-
 myristoyl-CoA wild type enzyme, at pH 7.5 and 25°C Sphingomonas paucimobilis
2.3.1.50 21
-
 palmitoyl-CoA wild type enzyme, at pH 7.5 and 25°C Sphingomonas paucimobilis