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Literature summary extracted from

  • Wang, L.; Suganuma, S.; Hibino, A.; Mitsui, R.; Tani, A.; Matsumoto, T.; Ebihara, A.; Fitriyanto, N.A.; Pertiwiningrum, A.; Shimada, M.; Hayakawa, T.; Nakagawa, T.
    Lanthanide-dependent methanol dehydrogenase from the legume symbiotic nitrogen-fixing bacterium Bradyrhizobium diazoefficiens strain USDA110 (2019), Enzyme Microb. Technol., 130, 109371 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.2.10 0.067
-
 methanol at pH 9.0 and 30°C Bradyrhizobium diazoefficiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.2.10 Ce3+ highest activity with 0.03 mM Ce3+. The enzyme contains 0.58 cerium atoms per subunit Bradyrhizobium diazoefficiens
1.1.2.10 La3+ second highest activity with 0.03 mM La3+ Bradyrhizobium diazoefficiens
1.1.2.10 Nd3+ activates at 0.03 mM Bradyrhizobium diazoefficiens
1.1.2.10 Pr3+ activates at 0.03 mM Bradyrhizobium diazoefficiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.2.10 136000
-
 calculated from SAXS data Bradyrhizobium diazoefficiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.10 Bradyrhizobium diazoefficiens
-
-
-
1.1.2.10 Bradyrhizobium diazoefficiens USDA110
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.10 ammonium sulfate precipitation, Toyopearl butyl-650S column chromatography and HiTrap column chromatography Bradyrhizobium diazoefficiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.2.10 culture condition:methanol-grown cell
-
 Bradyrhizobium diazoefficiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.10 1-butanol + phenazine ethosulfate 78.3% activity compared to methanol Bradyrhizobium diazoefficiens butyraldehyde + reduced phenazine ethosulfate
-
 r
1.1.2.10 1-butanol + phenazine ethosulfate 78.3% activity compared to methanol Bradyrhizobium diazoefficiens USDA110 butyraldehyde + reduced phenazine ethosulfate
-
 r
1.1.2.10 1-propanol + phenazine ethosulfate 85.3% activity compared to methanol Bradyrhizobium diazoefficiens propionaldehyde + reduced phenazine ethosulfate
-
 r
1.1.2.10 1-propanol + phenazine ethosulfate 85.3% activity compared to methanol Bradyrhizobium diazoefficiens USDA110 propionaldehyde + reduced phenazine ethosulfate
-
 r
1.1.2.10 2-propanol + phenazine ethosulfate 0.775% activity compared to methanol Bradyrhizobium diazoefficiens ?
-
 r
1.1.2.10 2-propanol + phenazine ethosulfate 0.775% activity compared to methanol Bradyrhizobium diazoefficiens USDA110 ?
-
 r
1.1.2.10 ethanol + phenazine ethosulfate 93% activity compared to methanol Bradyrhizobium diazoefficiens acetaldehyde + reduced phenazine ethosulfate
-
 r
1.1.2.10 ethanol + phenazine ethosulfate 93% activity compared to methanol Bradyrhizobium diazoefficiens USDA110 acetaldehyde + reduced phenazine ethosulfate
-
 r
1.1.2.10 formaldehyde + reduced phenazine ethosulfate 91.5% activity compared to methanol Bradyrhizobium diazoefficiens methanol + phenazine ethosulfate
-
 r
1.1.2.10 methanol + phenazine ethosulfate 100% activity Bradyrhizobium diazoefficiens formaldehyde + reduced phenazine ethosulfate
-
 r
1.1.2.10 methanol + phenazine ethosulfate 100% activity Bradyrhizobium diazoefficiens USDA110 formaldehyde + reduced phenazine ethosulfate
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.2.10 homodimer 2 * 64000, SDS-PAGE Bradyrhizobium diazoefficiens

Synonyms

EC Number Synonyms Comment Organism
1.1.2.10 lanthanides-dependent MDH
-
 Bradyrhizobium diazoefficiens
1.1.2.10 XoxF
-
 Bradyrhizobium diazoefficiens
1.1.2.10 XoxF5-type MDH
-
 Bradyrhizobium diazoefficiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.2.10 35
-
-
 Bradyrhizobium diazoefficiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.2.10 9
-
-
 Bradyrhizobium diazoefficiens