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Literature summary extracted from

  • Wang, W.; Su, S.; Wang, S.; Ye, L.; Yu, H.
    Significantly improved catalytic efficiency of caffeic acid O-methyltransferase towards N-acetylserotonin by strengthening its interactions with the unnatural substrates terminal structure (2019), Enzyme Microb. Technol., 125, 1-5 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.68 expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.68 C296F the mutant shows slight activity improvement in O-methylation of N-acetylserotonin compared to the wild type enzyme Arabidopsis thaliana
2.1.1.68 C296F/Q310L the mutant shows about 4.5fold activity improvement in O-methylation of N-acetylserotonin compared to the wild type enzyme Arabidopsis thaliana
2.1.1.68 C296F/Q310L/V314T the mutant shows 9.5fold activity improvement in O-methylation of N-acetylserotonin compared to the wild type enzyme Arabidopsis thaliana
2.1.1.68 C296H the mutant shows about 3fold activity improvement in O-methylation of N-acetylserotonin compared to the wild type enzyme Arabidopsis thaliana
2.1.1.68 C296H/Q310L the mutant shows about 3.5fold activity improvement in O-methylation of N-acetylserotonin compared to the wild type enzyme Arabidopsis thaliana
2.1.1.68 C296H/Q310L/V314T the mutant shows about 6.5fold activity improvement in O-methylation of N-acetylserotonin compared to the wild type enzyme Arabidopsis thaliana
2.1.1.68 C296Y the mutant shows about 2fold activity improvement in O-methylation of N-acetylserotonin compared to the wild type enzyme Arabidopsis thaliana
2.1.1.68 C296Y/Q310L/V314T the mutant shows about 8fold activity improvement in O-methylation of N-acetylserotonin compared to the wild type enzyme Arabidopsis thaliana
2.1.1.68 C296Y/V314T the mutant shows about 5.5fold activity improvement in O-methylation of N-acetylserotonin compared to the wild type enzyme Arabidopsis thaliana
2.1.1.68 Q310L the mutant shows about 2fold activity improvement in O-methylation of N-acetylserotonin compared to the wild type enzyme Arabidopsis thaliana
2.1.1.68 Q310L/V314T the mutant shows about 5.5fold activity improvement in O-methylation of N-acetylserotonin compared to the wild type enzyme Arabidopsis thaliana
2.1.1.68 V314N the mutant shows slight activity improvement in O-methylation of N-acetylserotonin compared to the wild type enzyme Arabidopsis thaliana
2.1.1.68 V314T the mutant shows about 2.5fold activity improvement in O-methylation of N-acetylserotonin compared to the wild type enzyme Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.68 0.26
-
 N-acetylserotonin mutant enzyme C296F/Q310L/V314T, at pH 7.8 and 37°C Arabidopsis thaliana
2.1.1.68 0.36
-
 N-acetylserotonin mutant enzyme Q310L, at pH 7.8 and 37°C Arabidopsis thaliana
2.1.1.68 0.58
-
 N-acetylserotonin mutant enzyme C296F, at pH 7.8 and 37°C Arabidopsis thaliana
2.1.1.68 0.58
-
 N-acetylserotonin mutant enzyme V314T, at pH 7.8 and 37°C Arabidopsis thaliana
2.1.1.68 0.74
-
 N-acetylserotonin wild type enzyme, at pH 7.8 and 37°C Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.68 Arabidopsis thaliana Q9FK25
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.68 HisTrap column chromatography Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.68 0.00609
-
 wild type enzyme, at pH 7.8 and 37°C Arabidopsis thaliana
2.1.1.68 0.00945
-
 mutant enzyme V314N, at pH 7.8 and 37°C Arabidopsis thaliana
2.1.1.68 0.00982
-
 mutant enzyme C296F, at pH 7.8 and 37°C Arabidopsis thaliana
2.1.1.68 0.01109
-
 mutant enzyme C296Y, at pH 7.8 and 37°C Arabidopsis thaliana
2.1.1.68 0.01131
-
 mutant enzyme Q310L, at pH 7.8 and 37°C Arabidopsis thaliana
2.1.1.68 0.01697
-
 mutant enzyme V314T, at pH 7.8 and 37°C Arabidopsis thaliana
2.1.1.68 0.01985
-
 mutant enzyme C296H, at pH 7.8 and 37°C Arabidopsis thaliana
2.1.1.68 0.02186
-
 mutant enzyme C296H/Q310L, at pH 7.8 and 37°C Arabidopsis thaliana
2.1.1.68 0.02701
-
 mutant enzyme C296F/Q310L, at pH 7.8 and 37°C Arabidopsis thaliana
2.1.1.68 0.03342
-
 mutant enzyme Q310L/V314T, at pH 7.8 and 37°C Arabidopsis thaliana
2.1.1.68 0.03462
-
 mutant enzyme C296A/V314T, at pH 7.8 and 37°C Arabidopsis thaliana
2.1.1.68 0.03913
-
 mutant enzyme C296H/Q310L/V314T, at pH 7.8 and 37°C Arabidopsis thaliana
2.1.1.68 0.04694
-
 mutant enzyme C296Y/Q310L/V314T, at pH 7.8 and 37°C Arabidopsis thaliana
2.1.1.68 0.05787
-
 mutant enzyme C296F/Q310L/V314T, at pH 7.8 and 37°C Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.68 S-adenosyl-L-methionine + N-acetylserotonin
-
 Arabidopsis thaliana S-adenosyl-L-homocysteine + melatonin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.68 caffeic acid O-methyltransferase
-
 Arabidopsis thaliana
2.1.1.68 COMT
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.68 2.94
-
 N-acetylserotonin wild type enzyme, at pH 7.8 and 37°C Arabidopsis thaliana
2.1.1.68 4.76
-
 N-acetylserotonin mutant enzyme Q310L, at pH 7.8 and 37°C Arabidopsis thaliana
2.1.1.68 5.41
-
 N-acetylserotonin mutant enzyme C296F, at pH 7.8 and 37°C Arabidopsis thaliana
2.1.1.68 11.32
-
 N-acetylserotonin mutant enzyme V314T, at pH 7.8 and 37°C Arabidopsis thaliana
2.1.1.68 43.79
-
 N-acetylserotonin mutant enzyme C296F/Q310L/V314T, at pH 7.8 and 37°C Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.68 3990
-
 N-acetylserotonin wild type enzyme, at pH 7.8 and 37°C Arabidopsis thaliana
2.1.1.68 9370
-
 N-acetylserotonin mutant enzyme C296F, at pH 7.8 and 37°C Arabidopsis thaliana
2.1.1.68 13110
-
 N-acetylserotonin mutant enzyme Q310L, at pH 7.8 and 37°C Arabidopsis thaliana
2.1.1.68 19530
-
 N-acetylserotonin mutant enzyme V314T, at pH 7.8 and 37°C Arabidopsis thaliana
2.1.1.68 171060
-
 N-acetylserotonin mutant enzyme C296F/Q310L/V314T, at pH 7.8 and 37°C Arabidopsis thaliana