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Literature summary extracted from

  • Rha, C.S.; Choi, J.M.; Jung, Y.S.; Kim, E.R.; Ko, M.J.; Seo, D.H.; Kim, D.O.; Park, C.S.
    High-efficiency enzymatic production of alpha-isoquercitrin glucosides by amylosucrase from Deinococcus geothermalis (2019), Enzyme Microb. Technol., 120, 84-90 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.4 synthesis isoquercitrin (quercetin-3-O-beta-D-glucopyranoside) has diverse biological functions, such as anti-oxidant and anticancer activity, but its use is limited by poor solubility and bioavailability. Enzymatically modified isoquercitrin (EMIQ) is a mixture of transglycosylated isoquercitrins that have better solubility and bioavailability than do quercetin and isoquercitrin. Amylosucrase (ASase), has transglycosylation activity to produce EMIQ. Both enzymes produce a variety of EMIQs including isoquercitrin, isoquercitrin-glucoside, isoquercitrin-diglucoside, and isoquercitrin-triglucoside. The enzyme has a higher bioconversion yield from isoquercitrin to EMIQ (97%). The yield of soquercitrin-triglucoside, which is the most bioavailable form is 46%. The enzyme can be used to synthesize EMIQ in a simple and specific process Deinococcus geothermalis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.4 expression in Escherichia coli Deinococcus geothermalis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.4 Deinococcus geothermalis Q1J0W0
-
-
2.4.1.4 Deinococcus geothermalis DSM 11300 Q1J0W0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.4 sucrose + isoquercetin isoquercitrin i.e. quercetin-3-O-beta-D-glucoside Deinococcus geothermalis D-fructose + isoquercitrin-glucoside
-
 ?
2.4.1.4 sucrose + isoquercetin isoquercitrin i.e. quercetin-3-O-beta-D-glucoside Deinococcus geothermalis DSM 11300 D-fructose + isoquercitrin-glucoside
-
 ?
2.4.1.4 sucrose + isoquercitrin-diglucoside isoquercitrin i.e. quercetin-3-O-beta-D-glucoside Deinococcus geothermalis D-fructose + isoquercitrin-triglucoside
-
 ?
2.4.1.4 sucrose + isoquercitrin-diglucoside isoquercitrin i.e. quercetin-3-O-beta-D-glucoside Deinococcus geothermalis DSM 11300 D-fructose + isoquercitrin-triglucoside
-
 ?
2.4.1.4 sucrose + isoquercitrin-glucoside isoquercitrin i.e. quercetin-3-O-beta-D-glucoside Deinococcus geothermalis D-fructose + isoquercitrin-diglucoside
-
 ?
2.4.1.4 sucrose + isoquercitrin-glucoside isoquercitrin i.e. quercetin-3-O-beta-D-glucoside Deinococcus geothermalis DSM 11300 D-fructose + isoquercitrin-diglucoside
-
 ?
2.4.1.4 sucrose + quercetin isoquercitrin i.e. quercetin-3-O-beta-D-glucoside Deinococcus geothermalis D-fructose + isoquercitrin
-
 ?
2.4.1.4 sucrose + quercetin isoquercitrin i.e. quercetin-3-O-beta-D-glucoside Deinococcus geothermalis DSM 11300 D-fructose + isoquercitrin
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.4 45
-
-
 Deinococcus geothermalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.4 7
-
-
 Deinococcus geothermalis