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Literature summary extracted from

  • Pol, A.; Barends, T.R.; Dietl, A.; Khadem, A.F.; Eygensteyn, J.; Jetten, M.S.; Op den Camp, H.J.
    Rare earth metals are essential for methanotrophic life in volcanic mudpots (2014), Environ. Microbiol., 16, 255-264 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.2.10 hanging drop vapor diffusion method, using 22% (w/v) PEG 8000, 0.2 M NaCl Methylacidiphilum fumariolicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.2.10 additional information not inhibited by cyanide Methylacidiphilum fumariolicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.2.10 0.0008
-
 methanol at pH 7.0 and 40-45°C Methylacidiphilum fumariolicum
1.1.2.10 0.002
-
 1-Hexanol at pH 7.0 and 40-45°C Methylacidiphilum fumariolicum
1.1.2.10 0.003
-
 ethanol at pH 7.0 and 40-45°C Methylacidiphilum fumariolicum
1.1.2.10 0.006
-
 1-butanol at pH 7.0 and 40-45°C Methylacidiphilum fumariolicum
1.1.2.10 0.007
-
 formaldehyde at pH 7.0 and 40-45°C Methylacidiphilum fumariolicum
1.1.2.10 0.007
-
 1-propanol at pH 7.0 and 40-45°C Methylacidiphilum fumariolicum
1.1.2.10 0.2
-
 acetaldehyde Km above 0.2 mM, at pH 7.0 and 40-45°C Methylacidiphilum fumariolicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.2.10 Ce3+ dependent on Methylacidiphilum fumariolicum
1.1.2.10 La3+ dependent on Methylacidiphilum fumariolicum
1.1.2.10 additional information the enzyme is completely independent of ammonium Methylacidiphilum fumariolicum
1.1.2.10 Nd3+ dependent on Methylacidiphilum fumariolicum
1.1.2.10 Pr3+ dependent on Methylacidiphilum fumariolicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.2.10 127000
-
 gel filtration Methylacidiphilum fumariolicum

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.10 Methylacidiphilum fumariolicum
-
-
-
1.1.2.10 Methylacidiphilum fumariolicum SolV
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.10 SP-Sepharose column chromatography Methylacidiphilum fumariolicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.10 1-butanol + phenazine ethosulfate 100% activity compared to methanol Methylacidiphilum fumariolicum butyraldehyde + reduced phenazine ethosulfate
-
 ?
1.1.2.10 1-butanol + phenazine ethosulfate 100% activity compared to methanol Methylacidiphilum fumariolicum SolV butyraldehyde + reduced phenazine ethosulfate
-
 ?
1.1.2.10 1-hexanol + phenazine ethosulfate 100% activity compared to methanol Methylacidiphilum fumariolicum hexaldehyde + reduced phenazine ethosulfate
-
 ?
1.1.2.10 1-hexanol + phenazine ethosulfate 100% activity compared to methanol Methylacidiphilum fumariolicum SolV hexaldehyde + reduced phenazine ethosulfate
-
 ?
1.1.2.10 1-propanol + phenazine ethosulfate 100% activity compared to methanol Methylacidiphilum fumariolicum propionaldehyde + reduced phenazine ethosulfate
-
 ?
1.1.2.10 acetaldehyde + reduced phenazine ethosulfate less than 50% activity compared to methanol Methylacidiphilum fumariolicum ethanol + phenazine ethosulfate
-
 ?
1.1.2.10 ethanol + phenazine ethosulfate 100% activity compared to methanol Methylacidiphilum fumariolicum acetaldehyde + reduced phenazine ethosulfate
-
 ?
1.1.2.10 formaldehyde + reduced phenazine ethosulfate 100% activity compared to methanol Methylacidiphilum fumariolicum methanol + phenazine ethosulfate
-
 r
1.1.2.10 methanol + phenazine ethosulfate 100% activity Methylacidiphilum fumariolicum formaldehyde + reduced phenazine ethosulfate
-
 r
1.1.2.10 methanol + phenazine ethosulfate 100% activity Methylacidiphilum fumariolicum SolV formaldehyde + reduced phenazine ethosulfate
-
 r
1.1.2.10 methanol + phenazine methosulfate 100% activity Methylacidiphilum fumariolicum formaldehyde + reduced phenazine methosulfate
-
 r
1.1.2.10 methanol + phenazine methosulfate 100% activity Methylacidiphilum fumariolicum SolV formaldehyde + reduced phenazine methosulfate
-
 r
1.1.2.10 additional information no activity with 2-propanol and formate Methylacidiphilum fumariolicum ?
-
-
1.1.2.10 additional information no activity with 2-propanol and formate Methylacidiphilum fumariolicum SolV ?
-
-

Subunits

EC Number Subunits Comment Organism
1.1.2.10 homodimer 2 * 60000, SDS-PAGE Methylacidiphilum fumariolicum
1.1.2.10 homodimer 2 * 63502, MALDI-TOF mass spectrometry Methylacidiphilum fumariolicum
1.1.2.10 homodimer 2 * 63586, calculated from amino acid sequence Methylacidiphilum fumariolicum

Synonyms

EC Number Synonyms Comment Organism
1.1.2.10 lanthanide-dependent MDH
-
 Methylacidiphilum fumariolicum
1.1.2.10 Ln-MDH
-
 Methylacidiphilum fumariolicum
1.1.2.10 XoxF-type MDH
-
 Methylacidiphilum fumariolicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.2.10 60
-
-
 Methylacidiphilum fumariolicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.2.10 7
-
-
 Methylacidiphilum fumariolicum