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Literature summary extracted from

  • Chen, C.; Liu, Z.; Liu, L.; Wang, J.; Jin, Q.
    Structural characterization of glycinamide-RNase-transformylase T from Mycobacterium tuberculosis (2020), Emerg. Microbes Infect., 9, 58-66 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.21 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.1.21 microbatch method, using 0.06 M sodium acetate, pH 4.6, 6% (w/v) PEG4000, 32% (v/v) glycerol Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
6.3.1.21 E217A the mutant loses its ATP-binding capability Mycobacterium tuberculosis
6.3.1.21 R131A the mutant loses its ATP-binding capability Mycobacterium tuberculosis
6.3.1.21 S218A the mutant loses its ATP-binding capability Mycobacterium tuberculosis
6.3.1.21 V220A the mutant loses its ATP-binding capability Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.21 90000
-
 gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.21 ATP + formate + N1-(5-phospho-D-ribosyl)glycinamide Mycobacterium tuberculosis
-
 ADP + H+ + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + phosphate
-
 ?
6.3.1.21 ATP + formate + N1-(5-phospho-D-ribosyl)glycinamide Mycobacterium tuberculosis H37Rv
-
 ADP + H+ + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.21 Mycobacterium tuberculosis P95197
-
-
6.3.1.21 Mycobacterium tuberculosis H37Rv P95197
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.21 Ni-NTA column chromatography, HQ20 column chromatography and Superdex S200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.21 ATP + formate + N1-(5-phospho-D-ribosyl)glycinamide
-
 Mycobacterium tuberculosis ADP + H+ + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + phosphate
-
 ?
6.3.1.21 ATP + formate + N1-(5-phospho-D-ribosyl)glycinamide
-
 Mycobacterium tuberculosis H37Rv ADP + H+ + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.1.21 homodimer 2 * 44000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
6.3.1.21 GAR transformylase
-
 Mycobacterium tuberculosis
6.3.1.21 glycinamide-RNase-transformylase T
-
 Mycobacterium tuberculosis
6.3.1.21 PurT
-
 Mycobacterium tuberculosis