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Literature summary extracted from
- Post-translational thioamidation of methyl-coenzyme M reductase, a key enzyme in methanogenic and methanotrophic Archaea (2017), eLife, 6, e29218 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.8.4.1 | recombinant expression of Strep-tagged enzyme | Methanosarcina barkeri |
| 2.8.4.1 | recombinant expression of Strep-tagged enzyme | Methanosarcina acetivorans |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.8.4.1 | Ni2+ | contained in the coenzyme F430 | Methanosarcina barkeri |  |
| 2.8.4.1 | Ni2+ | contained in the coenzyme F430 | Methanosarcina acetivorans | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.8.4.1 | methyl-CoM + CoB | Methanosarcina barkeri | - |
CoM-S-S-CoB + methane | - |
? | |
| 2.8.4.1 | methyl-CoM + CoB | Methanosarcina acetivorans | - |
CoM-S-S-CoB + methane | - |
? | |
| 2.8.4.1 | methyl-CoM + CoB | Methanosarcina acetivorans ATCC 35395 | - |
CoM-S-S-CoB + methane | - |
? | |
| 2.8.4.1 | methyl-CoM + CoB | Methanosarcina barkeri Fusaro | - |
CoM-S-S-CoB + methane | - |
? | |
| 2.8.4.1 | methyl-CoM + CoB | Methanosarcina acetivorans DSM 2834 | - |
CoM-S-S-CoB + methane | - |
? | |
| 2.8.4.1 | methyl-CoM + CoB | Methanosarcina acetivorans JCM 12185 | - |
CoM-S-S-CoB + methane | - |
? | |
| 2.8.4.1 | methyl-CoM + CoB | Methanosarcina barkeri DSM 804 | - |
CoM-S-S-CoB + methane | - |
? | |
| 6.2.2.1 | ATP + sulfide + a [methyl-coenzyme M reductase alpha-subunit McrA]-glycine465 | Methanosarcina acetivorans | - |
ADP + phosphate + a [methyl-coenzyme M reductase alpha-subunit McrA]-thioglycine465 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.8.4.1 | Methanosarcina acetivorans | Q8THH1 AND Q8THG7 AND Q8THH0 | subunits alpha, beta, and gamma | - |
| 2.8.4.1 | Methanosarcina acetivorans ATCC 35395 | Q8THH1 AND Q8THG7 AND Q8THH0 | subunits alpha, beta, and gamma | - |
| 2.8.4.1 | Methanosarcina acetivorans DSM 2834 | Q8THH1 AND Q8THG7 AND Q8THH0 | subunits alpha, beta, and gamma | - |
| 2.8.4.1 | Methanosarcina acetivorans JCM 12185 | Q8THH1 AND Q8THG7 AND Q8THH0 | subunits alpha, beta, and gamma | - |
| 2.8.4.1 | Methanosarcina barkeri | P07962 and P07955 and P07964 | subunits alpha, beta, and gamma | - |
| 2.8.4.1 | Methanosarcina barkeri DSM 804 | P07962 and P07955 and P07964 | subunits alpha, beta, and gamma | - |
| 2.8.4.1 | Methanosarcina barkeri Fusaro | P07962 and P07955 and P07964 | subunits alpha, beta, and gamma | - |
| 6.2.2.1 | Methanosarcina acetivorans | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 2.8.4.1 | additional information | hylogenetic analyses of methyltransferases TfuA and YcaO in methanogenic andmethanotrophic archaea | Methanosarcina acetivorans |
| 2.8.4.1 | side-chain modification | formation of thioglycine (Gly465), growth defects are associated with loss of the McrA thioglycine modification, mutations in ycaO and/or tfuA are responsible for loss of the thioglycine modification. Thioglycine does not influence the global stability of MCR | Methanosarcina acetivorans |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.8.4.1 | recombinant Strep-tagged enzyme by affinity chromatography | Methanosarcina barkeri |
| 2.8.4.1 | recombinant Strep-tagged enzyme by affinity chromatography | Methanosarcina acetivorans |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 2.8.4.1 | methyl-CoM + CoB = CoM-S-S-CoB + methane | the reaction proceeds via an unprecedented methyl radical intermediate | Methanosarcina barkeri | |
| 2.8.4.1 | methyl-CoM + CoB = CoM-S-S-CoB + methane | the reaction proceeds via an unprecedented methyl radical intermediate | Methanosarcina acetivorans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.8.4.1 | methyl-CoM + CoB | - |
Methanosarcina barkeri | CoM-S-S-CoB + methane | - |
? | |
| 2.8.4.1 | methyl-CoM + CoB | - |
Methanosarcina acetivorans | CoM-S-S-CoB + methane | - |
? | |
| 2.8.4.1 | methyl-CoM + CoB | - |
Methanosarcina acetivorans ATCC 35395 | CoM-S-S-CoB + methane | - |
? | |
| 2.8.4.1 | methyl-CoM + CoB | - |
Methanosarcina barkeri Fusaro | CoM-S-S-CoB + methane | - |
? | |
| 2.8.4.1 | methyl-CoM + CoB | - |
Methanosarcina acetivorans DSM 2834 | CoM-S-S-CoB + methane | - |
? | |
| 2.8.4.1 | methyl-CoM + CoB | - |
Methanosarcina acetivorans JCM 12185 | CoM-S-S-CoB + methane | - |
? | |
| 2.8.4.1 | methyl-CoM + CoB | - |
Methanosarcina barkeri DSM 804 | CoM-S-S-CoB + methane | - |
? | |
| 6.2.2.1 | ATP + sulfide + a [methyl-coenzyme M reductase alpha-subunit McrA]-glycine465 | - |
Methanosarcina acetivorans | ADP + phosphate + a [methyl-coenzyme M reductase alpha-subunit McrA]-thioglycine465 | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.8.4.1 | MCR | - |
Methanosarcina barkeri |
| 2.8.4.1 | MCR | - |
Methanosarcina acetivorans |
| 2.8.4.1 | methyl-coenzyme M reductase | - |
Methanosarcina barkeri |
| 2.8.4.1 | methyl-coenzyme M reductase | - |
Methanosarcina acetivorans |
| 6.2.2.1 | ycaO | - |
Methanosarcina acetivorans |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.8.4.1 | F-430 | coenzyme F430 | Methanosarcina barkeri | |
| 2.8.4.1 | F-430 | coenzyme F430 | Methanosarcina acetivorans | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 2.8.4.1 | malfunction | growth defects associated with loss of the McrA thioglycine modification. The DELTAycaO-tfuA mutant lacks the McrA Gly465 thioamide | Methanosarcina acetivorans |
| 2.8.4.1 | physiological function | methyl-coenzyme M reductase is a key enzyme in methanogenic and methanotrophic Archaea. Methyl-coenzyme M reductase (MCR) is a unique enzyme found exclusively in anaerobic archaea, where it catalyzes the reversible conversion of methyl-coenzyme M (CoM, 2-methylmercaptoethane-sulfonate) and coenzyme B (CoB, 7-thioheptanoylthreoninephosphate) to methane and a CoB-CoM heterodisulfide. The activity plays a critical role in the global carbon cycle | Methanosarcina barkeri |
| 2.8.4.1 | physiological function | methyl-coenzyme M reductase is a key enzyme in methanogenic and methanotrophic Archaea. Methyl-coenzyme M reductase (MCR) is a unique enzyme found exclusively in anaerobic archaea, where it catalyzes the reversible conversion of methyl-coenzyme M (CoM, 2-methylmercaptoethane-sulfonate) and coenzyme B (CoB, 7-thioheptanoylthreoninephosphate) to methane and a CoB-CoM heterodisulfide. The activity plays a critical role in the global carbon cycle | Methanosarcina acetivorans |
| 6.2.2.1 | malfunction | enzyme loss is associated with growth defects | Methanosarcina acetivorans |
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