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Literature summary extracted from

  • Kim, K.; Zheng, F.; Zhan, C.G.
    Oligomerization and catalytic parameters of human UDP-glucuronosyltransferase 1A10 expression and characterization of the recombinant protein (2018), Drug Metab. Dispos., 46, 1446-1452 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.17 expressed in HEK-293 and CHO cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.17 0.0457
-
 entacapone membrane-bound recombinant isoform UGT1A10 from CHO cells, at pH 7.4 and 37°C Homo sapiens
2.4.1.17 0.0569
-
 entacapone membrane-bound recombinant isoform UGT1A10 from HEK-293 cells, at pH 7.4 and 37°C Homo sapiens
2.4.1.17 0.554
-
 entacapone with phospholipid purified recombinant isoform UGT1A10 from CHO cells, at pH 7.4 and 37°C Homo sapiens
2.4.1.17 0.703
-
 entacapone without phospholipid purified recombinant isoform UGT1A10 from CHO cells, at pH 7.4 and 37°C Homo sapiens
2.4.1.17 7.3
-
 morphine membrane-bound recombinant isoform UGT1A10 from CHO cells, at pH 7.4 and 37°C Homo sapiens
2.4.1.17 10.4
-
 morphine membrane-bound recombinant isoform UGT1A10 from HEK-293 cells, at pH 7.4 and 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.17 endoplasmic reticulum
-
 Homo sapiens 5783
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.17 Mg2+ 5 mM used in assay conditions Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.17 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.17 HisPur cobalt resin column chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.17 gastrointestinal tract
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.17 entacapone + UDP-glucuronate
-
 Homo sapiens UDP + entacapone-3-O-glucuronide
-
 ?
2.4.1.17 morphine + UDP-glucuronate
-
 Homo sapiens UDP + morphine-3-glucuronide
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.17 ? x * 65000, His6-tagged recombinant enzyme, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.4.1.17 UDP-glucuronosyltransferase
-
 Homo sapiens
2.4.1.17 UGT1A10 isoform Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.17 0.0013
-
 entacapone without phospholipid purified recombinant isoform UGT1A10 from CHO cells, at pH 7.4 and 37°C Homo sapiens
2.4.1.17 0.0027
-
 entacapone with phospholipid purified recombinant isoform UGT1A10 from CHO cells, at pH 7.4 and 37°C Homo sapiens
2.4.1.17 0.015
-
 morphine membrane-bound recombinant isoform UGT1A10 from HEK-293 cells, at pH 7.4 and 37°C Homo sapiens
2.4.1.17 0.073
-
 entacapone membrane-bound recombinant isoform UGT1A10 from HEK-293 cells, at pH 7.4 and 37°C Homo sapiens
2.4.1.17 0.138
-
 morphine membrane-bound recombinant isoform UGT1A10 from CHO cells, at pH 7.4 and 37°C Homo sapiens
2.4.1.17 0.422
-
 entacapone membrane-bound recombinant isoform UGT1A10 from CHO cells, at pH 7.4 and 37°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.17 0.00144
-
 morphine membrane-bound recombinant isoform UGT1A10 from HEK-293 cells, at pH 7.4 and 37°C Homo sapiens
2.4.1.17 0.00187
-
 entacapone without phospholipid purified recombinant isoform UGT1A10 from CHO cells, at pH 7.4 and 37°C Homo sapiens
2.4.1.17 0.0048
-
 entacapone with phospholipid purified recombinant isoform UGT1A10 from CHO cells, at pH 7.4 and 37°C Homo sapiens
2.4.1.17 0.0188
-
 morphine membrane-bound recombinant isoform UGT1A10 from CHO cells, at pH 7.4 and 37°C Homo sapiens
2.4.1.17 1.285
-
 entacapone membrane-bound recombinant isoform UGT1A10 from HEK-293 cells, at pH 7.4 and 37°C Homo sapiens
2.4.1.17 9.22
-
 entacapone membrane-bound recombinant isoform UGT1A10 from CHO cells, at pH 7.4 and 37°C Homo sapiens