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Literature summary extracted from

  • Tao, X.; Su, L.; Wu, J.
    Current studies on the enzymatic preparation 2-O-alpha-D-glucopyranosyl-L-ascorbic acid with cyclodextrin glycosyltransferase (2019), Crit. Rev. Biotechnol., 39, 249-257 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.19 1.25
-
 beta-cyclodextrin at pH 5.0 and 35°C Anaerobranca gottschalkii
2.4.1.19 1.31
-
 beta-cyclodextrin at pH 5.0 and 35°C Niallia circulans
2.4.1.19 1.51
-
 beta-cyclodextrin at pH 5.5 and 37°C Paenibacillus macerans
2.4.1.19 18.3
-
 L-ascorbic acid at pH 5.0 and 35°C Niallia circulans
2.4.1.19 19.6
-
 L-ascorbic acid at pH 5.0 and 35°C Anaerobranca gottschalkii
2.4.1.19 25.5
-
 L-ascorbic acid at pH 5.5 and 37°C Paenibacillus macerans

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Alkalihalobacillus alcalophilus
-
-
-
2.4.1.19 Alkalihalobacillus alcalophilus 7-12
-
-
-
2.4.1.19 Anaerobranca gottschalkii
-
-
-
2.4.1.19 Bacillus sp. SK 13.002
-
-
-
2.4.1.19 Geobacillus stearothermophilus
-
-
-
2.4.1.19 Niallia circulans
-
-
-
2.4.1.19 Niallia circulans 251
-
-
-
2.4.1.19 Paenibacillus macerans
-
-
-
2.4.1.19 Paenibacillus sp.
-
-
-
2.4.1.19 Paenibacillus sp. JB-13
-
-
-
2.4.1.19 Paenibacillus sp. JK-12
-
-
-
2.4.1.19 Thermoanaerobacter sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 alpha-cyclodextrin + L-ascorbic acid
-
 Paenibacillus sp. 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 alpha-cyclodextrin + L-ascorbic acid highest activity Paenibacillus macerans 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 alpha-cyclodextrin + L-ascorbic acid 30% conversion rate Thermoanaerobacter sp. 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 alpha-cyclodextrin + L-ascorbic acid highest activity, 40% conversion rate Geobacillus stearothermophilus 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 amylose + L-ascorbic acid
-
 Geobacillus stearothermophilus 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 beta-cyclodextrin + L-ascorbic acid
-
 Geobacillus stearothermophilus 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 beta-cyclodextrin + L-ascorbic acid
-
 Paenibacillus sp. 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 beta-cyclodextrin + L-ascorbic acid 1% conversion rate Alkalihalobacillus alcalophilus 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 beta-cyclodextrin + L-ascorbic acid 30% conversion rate Anaerobranca gottschalkii 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 beta-cyclodextrin + L-ascorbic acid 37% conversion rate Niallia circulans 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 beta-cyclodextrin + L-ascorbic acid 9% conversion rate Paenibacillus macerans 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 beta-cyclodextrin + L-ascorbic acid 1% conversion rate Alkalihalobacillus alcalophilus 7-12 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 beta-cyclodextrin + L-ascorbic acid 37% conversion rate Niallia circulans 251 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 corn starch + L-ascorbic acid
-
 Paenibacillus macerans 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 corn starch + L-ascorbic acid
-
 Paenibacillus sp. 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 dextrin + L-ascorbic acid
-
 Geobacillus stearothermophilus 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 dextrin + L-ascorbic acid
-
 Paenibacillus macerans 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 dextrin + L-ascorbic acid highest activity, 17% conversion rate Paenibacillus sp. 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 gamma-cyclodextrin + L-ascorbic acid
-
 Geobacillus stearothermophilus 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 gamma-cyclodextrin + L-ascorbic acid
-
 Paenibacillus macerans 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 gamma-cyclodextrin + L-ascorbic acid
-
 Paenibacillus sp. 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 maltoheptaose + L-ascorbic acid
-
 Geobacillus stearothermophilus 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 maltoheptaose + L-ascorbic acid
-
 Paenibacillus sp. 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 maltohexaose + L-ascorbic acid
-
 Geobacillus stearothermophilus 2-O-alpha-D-glucopyranosyl-L-ascorbic acid + ?
-
 ?
2.4.1.19 maltopentaose + L-ascorbic acid
-
 Geobacillus stearothermophilus 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 maltose + L-ascorbic acid
-
 Geobacillus stearothermophilus 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 maltose + L-ascorbic acid
-
 Paenibacillus macerans 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 maltose + L-ascorbic acid
-
 Paenibacillus sp. 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 maltose + L-ascorbic acid 20% conversion rate Bacillus sp. SK 13.002 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 maltotetraose + L-ascorbic acid
-
 Geobacillus stearothermophilus 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 maltotriose + L-ascorbic acid
-
 Geobacillus stearothermophilus 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 maltotriose + L-ascorbic acid
-
 Paenibacillus sp. 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 additional information no activity with D-glucose as glycosyl donor Geobacillus stearothermophilus ?
-
-
2.4.1.19 additional information no activity with D-glucose as glycosyl donor Paenibacillus macerans ?
-
-
2.4.1.19 additional information no activity with D-glucose as glycosyl donor Paenibacillus sp. ?
-
-
2.4.1.19 potato starch + L-ascorbic acid
-
 Paenibacillus macerans 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 potato starch + L-ascorbic acid
-
 Paenibacillus sp. 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 rice starch + L-ascorbic acid
-
 Paenibacillus macerans 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 rice starch + L-ascorbic acid
-
 Paenibacillus sp. 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 soluble starch + L-ascorbic acid
-
 Paenibacillus sp. 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 soluble starch + L-ascorbic acid
-
 Paenibacillus sp. JK-12 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?
2.4.1.19 soluble starch + L-ascorbic acid
-
 Paenibacillus sp. JB-13 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.19 CGTase
-
 Geobacillus stearothermophilus
2.4.1.19 CGTase
-
 Niallia circulans
2.4.1.19 CGTase
-
 Paenibacillus macerans
2.4.1.19 CGTase
-
 Alkalihalobacillus alcalophilus
2.4.1.19 CGTase
-
 Thermoanaerobacter sp.
2.4.1.19 CGTase
-
 Paenibacillus sp.
2.4.1.19 CGTase
-
 Anaerobranca gottschalkii
2.4.1.19 CGTase
-
 Paenibacillus sp. JK-12
2.4.1.19 CGTase
-
 Paenibacillus sp. JB-13
2.4.1.19 CGTase
-
 Bacillus sp. SK 13.002
2.4.1.19 cyclodextrin glucosyltransferase
-
 Geobacillus stearothermophilus
2.4.1.19 cyclodextrin glucosyltransferase
-
 Niallia circulans
2.4.1.19 cyclodextrin glucosyltransferase
-
 Paenibacillus macerans
2.4.1.19 cyclodextrin glucosyltransferase
-
 Alkalihalobacillus alcalophilus
2.4.1.19 cyclodextrin glucosyltransferase
-
 Thermoanaerobacter sp.
2.4.1.19 cyclodextrin glucosyltransferase
-
 Paenibacillus sp.
2.4.1.19 cyclodextrin glucosyltransferase
-
 Anaerobranca gottschalkii
2.4.1.19 cyclodextrin glucosyltransferase
-
 Paenibacillus sp. JK-12
2.4.1.19 cyclodextrin glucosyltransferase
-
 Paenibacillus sp. JB-13
2.4.1.19 cyclodextrin glucosyltransferase
-
 Bacillus sp. SK 13.002
2.4.1.19 cyclomaltodextrin glycanotransferase
-
 Geobacillus stearothermophilus
2.4.1.19 cyclomaltodextrin glycanotransferase
-
 Niallia circulans
2.4.1.19 cyclomaltodextrin glycanotransferase
-
 Paenibacillus macerans
2.4.1.19 cyclomaltodextrin glycanotransferase
-
 Alkalihalobacillus alcalophilus
2.4.1.19 cyclomaltodextrin glycanotransferase
-
 Thermoanaerobacter sp.
2.4.1.19 cyclomaltodextrin glycanotransferase
-
 Paenibacillus sp.
2.4.1.19 cyclomaltodextrin glycanotransferase
-
 Anaerobranca gottschalkii
2.4.1.19 cyclomaltodextrin glycanotransferase
-
 Paenibacillus sp. JK-12
2.4.1.19 cyclomaltodextrin glycanotransferase
-
 Paenibacillus sp. JB-13
2.4.1.19 cyclomaltodextrin glycanotransferase
-
 Bacillus sp. SK 13.002
2.4.1.19 cyclomaltodextrin glycosyltransferase
-
 Geobacillus stearothermophilus
2.4.1.19 cyclomaltodextrin glycosyltransferase
-
 Niallia circulans
2.4.1.19 cyclomaltodextrin glycosyltransferase
-
 Paenibacillus macerans
2.4.1.19 cyclomaltodextrin glycosyltransferase
-
 Alkalihalobacillus alcalophilus
2.4.1.19 cyclomaltodextrin glycosyltransferase
-
 Thermoanaerobacter sp.
2.4.1.19 cyclomaltodextrin glycosyltransferase
-
 Paenibacillus sp.
2.4.1.19 cyclomaltodextrin glycosyltransferase
-
 Anaerobranca gottschalkii
2.4.1.19 cyclomaltodextrin glycosyltransferase
-
 Paenibacillus sp. JK-12
2.4.1.19 cyclomaltodextrin glycosyltransferase
-
 Paenibacillus sp. JB-13
2.4.1.19 cyclomaltodextrin glycosyltransferase
-
 Bacillus sp. SK 13.002

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.19 35
-
-
 Niallia circulans
2.4.1.19 35
-
-
 Anaerobranca gottschalkii
2.4.1.19 37
-
-
 Paenibacillus macerans
2.4.1.19 37
-
-
 Bacillus sp. SK 13.002
2.4.1.19 45
-
-
 Paenibacillus sp.
2.4.1.19 50
-
-
 Alkalihalobacillus alcalophilus
2.4.1.19 50
-
-
 Thermoanaerobacter sp.
2.4.1.19 55
-
-
 Paenibacillus sp. JK-12
2.4.1.19 55
-
-
 Paenibacillus sp. JB-13
2.4.1.19 60
-
-
 Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.19 4.5
-
-
 Thermoanaerobacter sp.
2.4.1.19 5
-
-
 Niallia circulans
2.4.1.19 5
-
-
 Anaerobranca gottschalkii
2.4.1.19 5.5
-
-
 Geobacillus stearothermophilus
2.4.1.19 5.5
-
-
 Paenibacillus macerans
2.4.1.19 5.5
-
-
 Paenibacillus sp. JK-12
2.4.1.19 6
-
-
 Paenibacillus sp.
2.4.1.19 6
-
-
 Paenibacillus sp. JB-13
2.4.1.19 7
-
-
 Alkalihalobacillus alcalophilus
2.4.1.19 8
-
-
 Bacillus sp. SK 13.002