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Literature summary extracted from

  • Li, R.; Zhu, L.N.; Ren, L.Q.; Weng, J.Y.; Sun, J.S.
    Molecular cloning and characterization of glycogen synthase in Eriocheir sinensis (2017), Comp. Biochem. Physiol. B, 214, 47-56 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.11 expressed in Escherichia coli Rosetta (DE3) cells Eriocheir sinensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.11 UDP-alpha-D-glucose + [(1->4)-alpha-D-glucosyl]n Eriocheir sinensis
-
 UDP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.11 Eriocheir sinensis
-
 Chinese mitten crab
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.11 Hi Trap column chromatography and Sepharose 6 column chromatography Eriocheir sinensis

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.4.1.11 using 50 mM Tris, 5 mM L(+)-cysteine, 8-1 M urea, pH 7.4 at 4°C for 4-6 h Eriocheir sinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.11 brain
-
 Eriocheir sinensis
-
2.4.1.11 gill
-
 Eriocheir sinensis
-
2.4.1.11 gut
-
 Eriocheir sinensis
-
2.4.1.11 heart
-
 Eriocheir sinensis
-
2.4.1.11 hepatopancreas highest expression Eriocheir sinensis
-
2.4.1.11 muscle
-
 Eriocheir sinensis
-
2.4.1.11 ovary
-
 Eriocheir sinensis
-
2.4.1.11 stomach
-
 Eriocheir sinensis
-
2.4.1.11 testis
-
 Eriocheir sinensis
-
2.4.1.11 thoracic ganglion
-
 Eriocheir sinensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.11 UDP-alpha-D-glucose + [(1->4)-alpha-D-glucosyl]n
-
 Eriocheir sinensis UDP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.11 UDP-glucose + glycogen
-
 Eriocheir sinensis glycogen + UDP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.11 ? x * 79000, calculated from amino acid sequence Eriocheir sinensis

Synonyms

EC Number Synonyms Comment Organism
2.4.1.11 glycogen synthase
-
 Eriocheir sinensis
2.4.1.11 GS-I
-
 Eriocheir sinensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.11 25
-
-
 Eriocheir sinensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.11 Eriocheir sinensis calculated from amino acid sequence
-
 5.9

Expression

EC Number Organism Comment Expression
2.4.1.11 Eriocheir sinensis enzyme expression and activity decline during moulting down

General Information

EC Number General Information Comment Organism
2.4.1.11 physiological function the enzyme is important for maintaining normal physiological functions such as growth and reproduction Eriocheir sinensis