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Literature summary extracted from

  • Fries, A.; Mazzaferro, L.S.; Gruening, B.; Bisel, P.; Stibal, K.; Buchholz, P.C.F.; Pleiss, J.; Sprenger, G.A.; Mueller, M.
    Alteration of the route to menaquinone towards isochorismate-derived metabolites (2019), ChemBioChem, 20, 1672-1677 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.2.1.9 N117R/L478T the mutant preferentially converts (5S,6S)-5,6-dihydroxycyclohexa-1,3-diene-1-carboxylate with a more than 70fold higher ratio than that for the wild type enzyme Escherichia coli
2.2.1.9 R107I the mutant uses (5S,6S)-6-amino-5-hydroxycyclohexa-1,3-diene-1-carboxylate as substrate with more than 6fold conversion compared to the wild type enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.9 isochorismate + 2-oxoglutarate Escherichia coli
-
 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.9 Escherichia coli P17109
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.9 (5S,6S)-5,6-dihydroxycyclohexa-1,3-diene-1-carboxylate + 2-oxoglutarate
-
 Escherichia coli (1R,2S,5S,6S)-2-succinyl-5,6-dihydroxycyclohex-3-ene-1-carboxylate + CO2
-
 ?
2.2.1.9 (5S,6S)-6-amino-5-hydroxycyclohexa-1,3-diene-1-carboxylate + 2-oxoglutarate
-
 Escherichia coli 2-succinyl-6-amino-5-hydroxycyclohex-3-ene-1-carboxylate + CO2
-
 ?
2.2.1.9 isochorismate + 2-oxoglutarate
-
 Escherichia coli 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.2.1.9 (1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxycyclohex-3-ene-1-carboxylate synthase
-
 Escherichia coli
2.2.1.9 MenD
-
 Escherichia coli
2.2.1.9 SEPHCHC synthase
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.9 thiamine diphosphate
-
 Escherichia coli