Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Luo, H.; Han, K.; Chang, Y.; Wang, Y.; Tong, S.; Nie, Z.; Wang, X.; Wei, Y.; Yu, H.; Shen, Z.
    Effects of inhibitors on the catalysis and immobilization of cephalosporin C acylase (2018), Chem. Biochem. Eng. Q., 32, 125-133 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.93 recombinant enzyme expression in Escherichia coli strain BL21(DE3)/pET-CPCAcy Pseudomonas sp. SE83

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.93 additional information the recombinant enzyme is immobilized, microenvironment with an acidic pH (about pH 6-7) during the catalysis of cephalosporin C with the immobilized enzyme. The immobilization efficiency is increased when the enzyme is immobilized in the presence of inhibitors sodium acetate and sodium bicarbobate. And the immobilized enzyme, in the presence of inhibitors, has a higher thermostability than the control at 50°C Pseudomonas sp. SE83

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.93 6-aminopenicillanic acid 6-APA, a noncompetitive inhibitor Pseudomonas sp. SE83
3.5.1.93 acetate
-
 Pseudomonas sp. SE83
3.5.1.93 bicarbonate
-
 Pseudomonas sp. SE83
3.5.1.93 Butyrate
-
 Pseudomonas sp. SE83
3.5.1.93 ethanolamine
-
 Pseudomonas sp. SE83
3.5.1.93 formate
-
 Pseudomonas sp. SE83
3.5.1.93 glucosamine
-
 Pseudomonas sp. SE83
3.5.1.93 Lactate
-
 Pseudomonas sp. SE83
3.5.1.93 additional information effects of the competitive inhibitors on the catalysis and immobilization of cephalosporin C acylase, mechanism of competitive inhibition, overview Pseudomonas sp. SE83
3.5.1.93 propionate
-
 Pseudomonas sp. SE83
3.5.1.93 Pyridine
-
 Pseudomonas sp. SE83

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.93 cephalosporin C + H2O Pseudomonas sp. SE83
-
 7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.93 Pseudomonas sp. SE83 P15557
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.93 cephalosporin C + H2O
-
 Pseudomonas sp. SE83 7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.93 AcyI
-
 Pseudomonas sp. SE83
3.5.1.93 acylase ACY 1 proenzyme UniProt Pseudomonas sp. SE83
3.5.1.93 CCA
-
 Pseudomonas sp. SE83
3.5.1.93 cephalosporin C acylase
-
 Pseudomonas sp. SE83

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.93 20
-
 recombinant immobilized enzyme, assay at Pseudomonas sp. SE83
3.5.1.93 37
-
 recombinant free enzyme, assay at Pseudomonas sp. SE83

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.93 50
-
 the immobilized enzyme, in the presence of inhibitors, has a higher thermostability than the control. The immobilized enzyme with sodium acetate shows a 31.7% increase in activity and a 25% increase with sodium bicarbonate. The increased stability and activity recovery in the process of immobilization might be attributed to the increase in the enzyme rigidity via binding of inhibitors with CCA at the active sites Pseudomonas sp. SE83

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.93 8
-
 recombinant free enzyme, assay at Pseudomonas sp. SE83
3.5.1.93 8.5
-
 recombinant immobilized enzyme, assay at Pseudomonas sp. SE83

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.93 additional information
-
 additional information inhibition kinetics Pseudomonas sp. SE83