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Literature summary extracted from

  • Beh, L.Y.; Debelouchina, G.T.; Clay, D.M.; Thompson, R.E.; Lindblad, K.A.; Hutton, E.R.; Bracht, J.R.; Sebra, R.P.; Muir, T.W.; Landweber, L.F.
    Identification of a DNA N6-adenine methyltransferase complex and its impact on chromatin organization (2019), Cell, 177, 1781-1796 .
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.72 macronucleus
-
 Tetrahymena thermophila 31039
-
2.1.1.72 macronucleus
-
 Oxytricha trifallax 31039
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Tetrahymena thermophila the enzyme consists of two MT-A70 proteins and two homeobox-like DNA-binding proteins and specifically methylates ApT dinucleotides in double stranded DNA. The enzyme activity is 3-3.5fold higher on hemimethylated substrates, relative to unmethylated double stranded DNA S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Oxytricha trifallax the enzyme consists of two MT-A70 proteins and two homeobox-like DNA-binding proteins and specifically methylates ApT dinucleotides in double stranded DNA. The enzyme activity is 3-3.5fold higher on hemimethylated substrates, relative to unmethylated double stranded DNA S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Oxytricha trifallax JRB310 the enzyme consists of two MT-A70 proteins and two homeobox-like DNA-binding proteins and specifically methylates ApT dinucleotides in double stranded DNA. The enzyme activity is 3-3.5fold higher on hemimethylated substrates, relative to unmethylated double stranded DNA S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Tetrahymena thermophila SB210 the enzyme consists of two MT-A70 proteins and two homeobox-like DNA-binding proteins and specifically methylates ApT dinucleotides in double stranded DNA. The enzyme activity is 3-3.5fold higher on hemimethylated substrates, relative to unmethylated double stranded DNA S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.72 Oxytricha trifallax
-
-
-
2.1.1.72 Oxytricha trifallax JRB310
-
-
-
2.1.1.72 Tetrahymena thermophila
-
-
-
2.1.1.72 Tetrahymena thermophila SB210
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.72 HiTrap Q column chromatography, HiTrap heparin column chromatography and Superdex 200 gel filtration Tetrahymena thermophila
2.1.1.72 HiTrap Q column chromatography, HiTrap heparin column chromatography and Superdex 200 gel filtration Oxytricha trifallax

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme consists of two MT-A70 proteins and two homeobox-like DNA-binding proteins and specifically methylates ApT dinucleotides in double stranded DNA. The enzyme activity is 3-3.5fold higher on hemimethylated substrates, relative to unmethylated double stranded DNA Tetrahymena thermophila S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme consists of two MT-A70 proteins and two homeobox-like DNA-binding proteins and specifically methylates ApT dinucleotides in double stranded DNA. The enzyme activity is 3-3.5fold higher on hemimethylated substrates, relative to unmethylated double stranded DNA Oxytricha trifallax S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme consists of two MT-A70 proteins and two homeobox-like DNA-binding proteins and specifically methylates ApT dinucleotides in double stranded DNA. The enzyme activity is 3-3.5fold higher on hemimethylated substrates, relative to unmethylated double stranded DNA Oxytricha trifallax JRB310 S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme consists of two MT-A70 proteins and two homeobox-like DNA-binding proteins and specifically methylates ApT dinucleotides in double stranded DNA. The enzyme activity is 3-3.5fold higher on hemimethylated substrates, relative to unmethylated double stranded DNA Tetrahymena thermophila SB210 S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.72 DNA 6mA methyltransferase
-
 Tetrahymena thermophila
2.1.1.72 DNA 6mA methyltransferase
-
 Oxytricha trifallax
2.1.1.72 MTA1 catalytic subunit Tetrahymena thermophila
2.1.1.72 MTA1 catalytic subunit Oxytricha trifallax
2.1.1.72 MTA1c
-
 Tetrahymena thermophila
2.1.1.72 MTA1c
-
 Oxytricha trifallax

General Information

EC Number General Information Comment Organism
2.1.1.72 malfunction enzyme gene disruption mutants fail to complete the sexual cycle when induced to mate and display complete lethality Tetrahymena thermophila
2.1.1.72 malfunction enzyme gene disruption mutants fail to complete the sexual cycle when induced to mate and display complete lethality Oxytricha trifallax