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Literature summary extracted from

  • Lin, J.; Gagnon, M.G.; Bulkley, D.; Steitz, T.A.
    Conformational changes of elongation factor G on the ribosome during tRNA translocation (2015), Cell, 160, 219-227 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.5.3 gene fusA, recombinant expression of the N-terminal domain (74 residues) of Thermus thermophilus ribosomal protein L9 fused to the N-terminus of EF-G connecting residues 1-74 of ribosomal protein L9 and residues 8-691 of EF-G in Escherichia coli strain BL21(DE3) Star Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.5.3 purified ribosomal protein L9-elongation factor G fusion protein in complex with GDP, the EF-G-GTP complex is bound on a pre-translocation ribosome (PRE ribosome) that is deficient in tRNA translocation upon EF-G binding, sitting drop vapour diffusion method, mixing of 0.1 mM protein in 10 mM Tris-HCl, pH 7.5, 200 mM KCl, 10 mM Mg(CH3COO)2, and 1 mM 2-mercaptoethanol, with X-ray diffraction structure determination and analysis at 2.0 A resolution. Only in the presence of the GDP nucleotide, and not GTP, does EF-G crystalize with the non-rotated ribosome under the experimental conditions Thermus thermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.5.3 ribosome
-
 Thermus thermophilus 5840
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.5.3 Mg2+ required Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.5.3 GTP + H2O Thermus thermophilus
-
 GDP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.5.3 Thermus thermophilus P13551
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.5.3 recombinant enzyme from Escherichia coli strain BL21(DE3) Star by hydrophobic interaction, anion exchange chromatography, gel filtration Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.5.3 GTP + H2O
-
 Thermus thermophilus GDP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.5.3 EF-G
-
 Thermus thermophilus
3.6.5.3 elongation factor G
-
 Thermus thermophilus
3.6.5.3 fusA
-
 Thermus thermophilus
3.6.5.3 GTPase
-
 Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.5.3 7.5
-
 assay at Thermus thermophilus

General Information

EC Number General Information Comment Organism
3.6.5.3 additional information EF-G binds with GTP or GDP on the PRE ribosome. But only in the presence of the GDP nucleotide, and not GTP, does EF-G crystalize with the non-rotated ribosome under the experimental conditions Thermus thermophilus
3.6.5.3 physiological function EF-G-mediated tRNA translocation, mechanism, overview Thermus thermophilus