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Literature summary extracted from

  • Zienkiewicz, K.; Benning, U.; Siegler, H.; Feussner, I.
    The type 2 acyl-CoA diacylglycerol acyltransferase family of the oleaginous microalga Lobosphaera incisa (2018), BMC Plant Biol., 18, 298 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.20 gene DGAT1, single copy gene, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis of DGAT isozymes, recombinant expression in the TAG-deficient Saccharomyces cerevisiae strain H1246, LiDGATs of type 1 and 2 do not co-localize with each other at least in yeast Lobosphaera incisa
2.3.1.20 gene DGAT2.1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis of DGAT isozymes, recombinant expression in the TAG-deficient Saccharomyces cerevisiae strain H1246, LiDGATs of type 1 and 2 do not co-localize with each other at least in yeast Lobosphaera incisa

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.20 additional information the TAG-deficient Saccharomyces cerevisiae strain H1246 is complemented with LiDGAT1, LiDGAT2.1, LiDGAT2.2 and LiDGAT2.3 as well as with their double constructs followed by analysis of synthesized TAGs Lobosphaera incisa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.20 endoplasmic reticulum the DGAT2 isozymes all localize to distinct endoplasmic reticulum domains Lobosphaera incisa 5783
-
2.3.1.20 endoplasmic reticulum the DGAT2 isozymes all localize to distinct ER domains Lobosphaera incisa 5783
-
2.3.1.20 membrane integral transmembrane enzyme Lobosphaera incisa 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Lobosphaera incisa
-
 CoA + triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Lobosphaera incisa
-
 CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Lobosphaera incisa SAG 2468
-
 CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Lobosphaera incisa SAG 2468
-
 CoA + triacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Lobosphaera incisa A0A2H4UKL3 the oleaginous microalgae has been first isolated from a glacier in Japan and named Myrmecia incisa to be renamed later to Parietochloris incisa in 2005
-
2.3.1.20 Lobosphaera incisa A0A386RTI5 the oleaginous microalgae has been first isolated from a glacier in Japan and named Myrmecia incisa to be renamed later to Parietochloris incisa in 2005
-
2.3.1.20 Lobosphaera incisa A0A386RU68 the oleaginous microalgae has been first isolated from a glacier in Japan and named Myrmecia incisa to be renamed later to Parietochloris incisa in 2005
-
2.3.1.20 Lobosphaera incisa A0A3Q8PZB6 the oleaginous microalgae has been first isolated from a glacier in Japan and named Myrmecia incisa to be renamed later to Parietochloris incisa in 2005
-
2.3.1.20 Lobosphaera incisa SAG 2468 A0A2H4UKL3 the oleaginous microalgae has been first isolated from a glacier in Japan and named Myrmecia incisa to be renamed later to Parietochloris incisa in 2005
-
2.3.1.20 Lobosphaera incisa SAG 2468 A0A386RTI5 the oleaginous microalgae has been first isolated from a glacier in Japan and named Myrmecia incisa to be renamed later to Parietochloris incisa in 2005
-
2.3.1.20 Lobosphaera incisa SAG 2468 A0A386RU68 the oleaginous microalgae has been first isolated from a glacier in Japan and named Myrmecia incisa to be renamed later to Parietochloris incisa in 2005
-
2.3.1.20 Lobosphaera incisa SAG 2468 A0A3Q8PZB6 the oleaginous microalgae has been first isolated from a glacier in Japan and named Myrmecia incisa to be renamed later to Parietochloris incisa in 2005
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Lobosphaera incisa CoA + triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Lobosphaera incisa SAG 2468 CoA + triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Lobosphaera incisa CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Lobosphaera incisa SAG 2468 CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 additional information recombinant isozyme LiDGAT2.2 shows significant triacylglycerol (TAG) production in response to exogenous arachidonic acid Lobosphaera incisa ?
-
-
2.3.1.20 additional information recombinant isozyme LiDGAT2.2 shows significant triacylglycerol (TAG) production in response to exogenous arachidonic acid Lobosphaera incisa SAG 2468 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.3.1.20 acyl-CoA:1,2-diacylglycerol O-acyltransferase UniProt Lobosphaera incisa
2.3.1.20 DGAT
-
 Lobosphaera incisa
2.3.1.20 DGAT1
-
 Lobosphaera incisa
2.3.1.20 DGAT2.1
-
 Lobosphaera incisa
2.3.1.20 DGAT2.2
-
 Lobosphaera incisa
2.3.1.20 DGAT2.3
-
 Lobosphaera incisa
2.3.1.20 DGAT2A
-
 Lobosphaera incisa
2.3.1.20 DGAT2b
-
 Lobosphaera incisa
2.3.1.20 type 1 acyl-CoA:diacylglycerol acyltransferase
-
 Lobosphaera incisa
2.3.1.20 type 2 acyl-CoA:diacylglycerol acyltransferase
-
 Lobosphaera incisa

General Information

EC Number General Information Comment Organism
2.3.1.20 evolution genome analysis reveals the presence of five putative DGAT isoforms in Lobosphaera incisa, including one DGAT of type 1, three DGATs of type 2 (isozymes LiDGAT2.1, LiDGAT2.2, and LiDGAT2.3), and a single isoform of a type 3 DGAT. Five DGATs encoded by the Lobosphaera incisa genome cluster differently within the eukaryotic DGAT family Lobosphaera incisa
2.3.1.20 evolution genome analysis reveals the presence of five putative DGAT isoforms in Lobosphaera incisa, including one DGAT of type 1, three DGATs of type 2, i.e. isozymes LiDGAT2.1, LiDGAT2.2, and LiDGAT2.3, and a single isoform of a type 3 DGAT. Five DGATs encoded by the Lobosphaera incisa genome cluster differently within the eukaryotic DGAT family Lobosphaera incisa
2.3.1.20 metabolism DGAT is considered as rate-limiting enzyme of TAG synthesis and accumulation in animals, plants and microbes Lobosphaera incisa
2.3.1.20 additional information in silico analyses of Lobosphaera incisa transcriptome data reveal 3 isoforms of DGAT type 2 Lobosphaera incisa
2.3.1.20 physiological function analysis of functional and cellular nature of type 1 and type 2 DGATs from Lobosphaera incisa, with LiDGAT1 being a major contributor to the TAG pool. LiDGATs of type 2 might be in turn involved in the incorporation of unusual fatty acids into TAG and thus regulate the composition of TAG Lobosphaera incisa