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Literature summary extracted from

  • Sonbol, S.A.; Ferreira, A.J.; Siam, R.
    Red Sea Atlantis II brine pool nitrilase with unique thermostability profile and heavy metal tolerance (2016), BMC Biotechnol., 16, 14 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.5.1 industry the purified enzyme reveales its selectivity towards dinitriles, which suggests a possible industrial application in the synthesis of cyanocarboxylic acids uncultured Burkholderiaceae bacterium
3.5.5.1 synthesis the purified enzyme reveales its selectivity towards dinitriles, which suggests a possible industrial application in the synthesis of cyanocarboxylic acids uncultured Burkholderiaceae bacterium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.1 expression into Escherichia coli BL21 (DE3) uncultured Burkholderiaceae bacterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.5.1 CdCl2 weak inhibition uncultured Burkholderiaceae bacterium
3.5.5.1 CoCl2 weak inhibition uncultured Burkholderiaceae bacterium
3.5.5.1 Cu2+ strong inhibition uncultured Burkholderiaceae bacterium
3.5.5.1 Hg2+ strong inhibition uncultured Burkholderiaceae bacterium
3.5.5.1 additional information the enzyme retains near maximal activity even in the presence of high concentrations of ZnCl2, MgSO4 and MnSO4 uncultured Burkholderiaceae bacterium
3.5.5.1 NaCl the enzyme loses more than 75% of its activity at NaCl concentrations higher than 2.0 mol/l uncultured Burkholderiaceae bacterium
3.5.5.1 NiSO4 weak inhibition uncultured Burkholderiaceae bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.5.1 59.4
-
Succinonitrile pH 7.2, 40°C uncultured Burkholderiaceae bacterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.5.1 38830
-
calculated from sequence uncultured Burkholderiaceae bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Cereibacter sphaeroides G5DDB2
-
-
3.5.5.1 Cereibacter sphaeroides LHS-305 G5DDB2
-
-
3.5.5.1 uncultured Burkholderiaceae bacterium A0A140D8I8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.1 His-tagged enzyme uncultured Burkholderiaceae bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 glutaronitrile + H2O activity towards glutaronitrile is 8.5% of that towards succinonitrile uncultured Burkholderiaceae bacterium glutaric acid + NH3
-
?
3.5.5.1 additional information no activity with mandelonitrile, arylacetonitrile and benzonitrile. No activity with acetamide and benzamide uncultured Burkholderiaceae bacterium ?
-
-
3.5.5.1 succinonitrile + H2O
-
uncultured Burkholderiaceae bacterium succinic acid + NH3
-
?

Synonyms

EC Number Synonyms Comment Organism
3.5.5.1 NitraS-ATII
-
Cereibacter sphaeroides
3.5.5.1 NitraS-ATII
-
uncultured Burkholderiaceae bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.5.1 35 40
-
uncultured Burkholderiaceae bacterium
3.5.5.1 50
-
-
Cereibacter sphaeroides

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.5.1 30 45 30°C: about 65% of maximal activity, 45°C: about 25% of maximal activity uncultured Burkholderiaceae bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.5.1 35
-
1 min, about 10% loss of activity uncultured Burkholderiaceae bacterium
3.5.5.1 50
-
1 min, about 25% loss of activity. 60 min, about 85% loss of activity uncultured Burkholderiaceae bacterium
3.5.5.1 55
-
1 min, about 25% loss of activity uncultured Burkholderiaceae bacterium
3.5.5.1 60
-
1 min, about 40% loss of activity uncultured Burkholderiaceae bacterium
3.5.5.1 65
-
1 min, about 40% loss of activity uncultured Burkholderiaceae bacterium
3.5.5.1 70
-
1 min, about 40% loss of activity uncultured Burkholderiaceae bacterium
3.5.5.1 75
-
1 min, about 70% loss of activity uncultured Burkholderiaceae bacterium
3.5.5.1 80
-
1 min, about 95% loss of activity uncultured Burkholderiaceae bacterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.5.1 0.472
-
Succinonitrile pH 7.2, 40°C uncultured Burkholderiaceae bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.1 7
-
-
uncultured Burkholderiaceae bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.5.1 5 9 pH 5.0: about 40% of maximal activity, pH 9.0: about 55% of maximal activity uncultured Burkholderiaceae bacterium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.5.1 0.0079
-
Succinonitrile pH 7.2, 40°C uncultured Burkholderiaceae bacterium