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Literature summary extracted from

  • Weber, N.; Ismail, A.; Gorwa-Grauslund, M.; Carlquist, M.
    Biocatalytic potential of vanillin aminotransferase from Capsicum chinense (2014), BMC Biotechnol., 14, 25 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.119 expressed in Escherichia coli BL21(DE3) pLys cells Capsicum chinense

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.119 L-alanine + vanillin Capsicum chinense
-
 pyruvate + vanillylamine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.119 Capsicum chinense O82521
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.119 Ni-NTA column chromatography Capsicum chinense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.119 (S)-1-phenylethylamine + 4-hydroxybenzaldehyde about 20% activity compared to pyruvate Capsicum chinense ?
-
 r
2.6.1.119 (S)-1-phenylethylamine + oxaloacetate about 90% activity compared to pyruvate Capsicum chinense acetophenone + ?
-
 r
2.6.1.119 (S)-1-phenylethylamine + pyruvate 100% activity Capsicum chinense acetophenone + ?
-
 r
2.6.1.119 L-alanine + vanillin
-
 Capsicum chinense pyruvate + vanillylamine
-
 r
2.6.1.119 additional information the enzyme has no activity with (R)-1-phenylethylamine. The enzyme shows no activity with succinate, acetone and 2-oxoglutarate as co-substrate Capsicum chinense ?
-
-

Subunits

EC Number Subunits Comment Organism
2.6.1.119 ? x * 57500, His-tagged enzyme, SDS-PAGE Capsicum chinense

Synonyms

EC Number Synonyms Comment Organism
2.6.1.119 PAMT
-
 Capsicum chinense
2.6.1.119 VAMT
-
 Capsicum chinense

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.119 8
-
-
 Capsicum chinense

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.119 6 9 more than 50% activity at pH 6.0-9.0 Capsicum chinense