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Literature summary extracted from

  • Dong, M.; Gonzalez, T.; Klems, M.; Steinberg, L.; Chen, W.; Papoutsakis, E.; Bahnson, B.
    In vitro methanol production from methyl coenzyme M using the Methanosarcina barkeri MtaABC protein complex (2017), Biotechnol. Prog., 33, 1243-1249 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.246 biofuel production demonstration of an in vitro ability of MtaABC to produce methanol may ultimately enable the anaerobic oxidation of methane to produce methanol and from methanol alternative fuel or fuel-precursor molecules Methanosarcina barkeri
2.1.1.246 synthesis in vitro methanol production from methyl coenzyme M using the Methanosarcina barkeri MtaABC protein complex Methanosarcina barkeri

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.90 expressed in Escherichia coli BL21 cells Methanosarcina barkeri
2.1.1.246 gene mtaA, recombinant expression as enzyme complex Methanosarcina barkeri MtaABC in Escherichia coli, recombinant expression of His-tagged MtaA in Escherichia coli Methanosarcina barkeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.246 additional information
-
 additional information kinetics Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.90 methanol + cob(I)alamin Methanosarcina barkeri
-
 methyl-cob(III)alamin + H2O
-
 r
2.1.1.90 methanol + cob(I)alamin Methanosarcina barkeri DSM 804
-
 methyl-cob(III)alamin + H2O
-
 r
2.1.1.246 a [methyl-Co(III) methanol-specific corrinoid protein] + CoM Methanosarcina barkeri
-
 methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.90 Methanosarcina barkeri Q48949 and Q46EH3 and Q46EH4 subunits MtaA and MtaB and MtaC
-
2.1.1.90 Methanosarcina barkeri DSM 804 Q48949 and Q46EH3 and Q46EH4 subunits MtaA and MtaB and MtaC
-
2.1.1.246 Methanosarcina barkeri Q48949
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.90 Ni-NTA column chromatography and Q Sepharose column chromatography Methanosarcina barkeri
2.1.1.246 recombinant His-tagged MtaA from Escherichia coli by nickel affinity chromatography, dialysis, and anion exchange chromatography Methanosarcina barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.90 methanol + cob(I)alamin
-
 Methanosarcina barkeri methyl-cob(III)alamin + H2O
-
 r
2.1.1.90 methanol + cob(I)alamin
-
 Methanosarcina barkeri DSM 804 methyl-cob(III)alamin + H2O
-
 r
2.1.1.246 a [methyl-Co(III) methanol-specific corrinoid protein] + CoM
-
 Methanosarcina barkeri methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
-
 ?
2.1.1.246 a [methyl-Co(III) methanol-specific corrinoid protein] + CoM
-
 Methanosarcina barkeri methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
-
 r
2.1.1.246 additional information the reaction for possible production of methanol from the anaerobic oxidation of methane can be reversed in vitro. Develoment of an in vitro functional assay that demonstrates MtaABC can catalyze the energetically unfavorable reverse reaction starting from methyl coenzyme M and generating methanol as a product, overview. MtaABC enzyme protein complex to catalyze the methyl transfer from methanol to CoM to form methyl-CoM, which is energetically favorable in the forward reaction. MtaB catalyzes a methyl transfer from methanol to the corrinoid cofactor of the MtaC subunit. MtaA then catalyzes the transfer of the methyl group to CoM to form methyl-CoM. DTNB assays for the forward and reverse MtaABC reactions. Methylcobalamin assay of purified recombinant MtaA.Demethylation of methylcobalamin in a CoM-dependent manner by MtaA Methanosarcina barkeri ?
-
-

Subunits

EC Number Subunits Comment Organism
2.1.1.90 ? x * 49000 + x * 36000 + x * 24000, SDS-PAGE Methanosarcina barkeri
2.1.1.246 ? x * 36000, recombinant His-tagged MtaA, SDS-PAGE Methanosarcina barkeri

Synonyms

EC Number Synonyms Comment Organism
2.1.1.90 methanol:coenzyme M methyltransferase
-
 Methanosarcina barkeri
2.1.1.90 methanol:CoM methyltransferase complex
-
 Methanosarcina barkeri
2.1.1.90 MtaABC
-
 Methanosarcina barkeri
2.1.1.246 mtaA
-
 Methanosarcina barkeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.246 7.2
-
 assay at Methanosarcina barkeri

General Information

EC Number General Information Comment Organism
2.1.1.246 additional information enzyme complex structure analysis and structure-function analysis, overview Methanosarcina barkeri
2.1.1.246 physiological function methanol:coenzyme M methyltransferase is an enzyme complex composed of three subunits, MtaA, MtaB, and MtaC, found in methanogenic archaea and is needed for their growth on methanol ultimately producing methane. MtaABC catalyzes the energetically favorable methyl transfer from methanol to coenzyme M to form methyl coenzyme M, an important reaction for possible production of methanol from the anaerobic oxidation of methane Methanosarcina barkeri